Results 41 to 50 of about 16,798,617 (404)
Microsystems for Cell Cultures
Microfabricated systems are increasingly being utilized in biotechnological, biomedical, and pharmaceutical research and development as replacements for traditional in vitro cell cultures, bioreactors, and animal experiments (Figure 1) [...]
openaire +3 more sources
Hepatitis C virus cell-cell transmission and resistance to direct-acting antiviral agents [PDF]
Hepatitis C virus (HCV) is transmitted between hepatocytes via classical cell entry but also uses direct cell-cell transfer to infect neighboring hepatocytes.
A Owsianka+72 more
core +6 more sources
Cell isolation and culture [PDF]
Cell isolation and culture are essential tools for the study of cell function. Isolated cells grown under controlled conditions can be manipulated and imaged at a level of resolution that is not possible in whole animals or even tissue explants. Recent advances have allowed for large-scale isolation and culture of primary C.
Sihui Zhang, Jeffrey R. Kuhn
openaire +3 more sources
A quantitative method to determine osteogenic differentiation aptness of scaffold
Osteogenic differentiation of Mesenchymal stem cells (MSCs) on scaffold is crucial for bone tissue engineering. Alkaline phosphatase (ALP) assay is an important method of assessing osteogenesis.
Shilpa Trivedi+6 more
doaj +1 more source
Type 2 diabetes mellitus (T2DM) is a growing global public health issue, and dipeptidyl peptidase-4 (DPP-4) is a potential therapeutic target in T2DM. Several synthetic anti-DPP-4 medications can be used to treat T2DM. However, because of adverse effects,
Sibhghatulla Shaikh+17 more
doaj +1 more source
Transcriptional landscape of epithelial and immune cell populations revealed through FACS-seq of healthy human skin. [PDF]
Human skin consists of multiple cell types, including epithelial, immune, and stromal cells. Transcriptomic analyses have previously been performed from bulk skin samples or from epithelial and immune cells expanded in cell culture.
Ahn, Richard S+9 more
core +1 more source
Scaffold-based 3D cell culture models in cancer research
Three-dimensional (3D) cell cultures have emerged as valuable tools in cancer research, offering significant advantages over traditional two-dimensional (2D) cell culture systems.
Waad H. Abuwatfa, W. Pitt, G. Husseini
semanticscholar +1 more source
Optimized exosome isolation protocol for cell culture supernatant and human plasma
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of disease. However, there is currently limited information elucidating the most efficient methods for obtaining high yields of pure exosomes, a subset of ...
R. Lobb+6 more
semanticscholar +1 more source
Genomics, proteomics, vaccinology, transgenics, stem cell—advances in all these areas critically stack on the shoulders of tissue culture, our ability to cultivate an organism's living cells in plastic dishes. Nutritional trial and error for decades of painstaking cell gardening laid the groundwork for the several thousand human primary cell explants ...
openaire +3 more sources
Modifications at the C-Terminus To Improve Pyrrole−Imidazole Polyamide Activity in Cell Culture [PDF]
Pyrrole−imidazole (Py-Im) hairpin polyamides are a class of small molecule DNA minor groove binding compounds that have been shown to modulate endogenous gene expression in cell culture.
Dervan, Peter B., Jacobs, Claire S.
core +2 more sources