Results 61 to 70 of about 3,334,633 (288)

Data-Analytics Modeling of Electrical Impedance Measurements for Cell Culture Monitoring [PDF]

open access: yes, 2019
High-throughput data analysis challenges in laboratory automation and lab-on-a-chip devices’ applications are continuously increasing. In cell culture monitoring, specifically, the electrical cell-substrate impedance sensing technique (ECIS), has been ...
Díaz, Roberto   +5 more
core   +2 more sources

Characterization of fungal carbonyl sulfide hydrolase belonging to clade D β‐carbonic anhydrase

open access: yesFEBS Letters, EarlyView.
Here, we performed a functional analysis of the fungal enzymes belonging to clade D of the β‐class carbonic anhydrase family (β‐D‐CA). The β‐D‐CAs in the basidiomycete Gloeophyllum trabeum and the ascomycete Trichoderma harzianum showed very low activity in the hydration of CO2 but exhibited high activity in the hydrolysis of carbonyl sulfide (COS ...
Ryuka Iizuka   +6 more
wiley   +1 more source

Three-dimensional tissue scaffolds from interbonded poly(e-caprolactone) fibrous matrices with controlled porosity

open access: yes, 2011
In this article, we report on the preparation and cell culture performance of a novel fibrous matrix that has an interbonded fiber architecture, excellent pore interconnectivity, and controlled pore size and porosity.
Kirkland, Mark   +6 more
core   +1 more source

A histidine‐rich extension of the mitochondrial F0 subunit ATP6 from the ice worm Mesenchytraeus solifugus increases ATP synthase activity in bacteria

open access: yesFEBS Letters, EarlyView.
The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley   +2 more
wiley   +1 more source

Expression of hepatitis B small surface antigen in Santalum album embryogenic cell suspension cultures

open access: yesBiologia Plantarum, 2010
Embryogenic cell suspension cultures of Santalum album were transformed with Agrobacterium tumefaciens harboring pD35SHER plant expression vector having hepatitis B small surface antigen (HBsAg) with a C-terminal ER retention signal.
U. K. S. Shekhawat   +2 more
doaj   +1 more source

سندرم متابولیک و COVID-19: چالش‌ها و عوارض بالینی

open access: yesمجله دانشکده پزشکی اصفهان, 2021
فشار خون بالا، مقاومت به انسولین، تری‌گلیسیرید بالا، کاهش لیپوپروتئین با چگالی بالا و چاقی احشایی، از مهم‌ترین شاخص‌های سندرم متابولیک هستند. سندروم متابولیک، مجموعه‌ای از شرایطی است که احتمال ابتلا به بیماری‌های قلبی- عروقی و مرگ و میر را افزایش می‌دهد.
Fereshteh Sadat Rezaei   +7 more
doaj   +1 more source

Distinguishing cancerous from non-cancerous cells through analysis of electrical noise [PDF]

open access: yes, 2007
Since 1984, electric cell-substrate impedance sensing (ECIS) has been used to monitor cell behavior in tissue culture and has proven sensitive to cell morphological changes and cell motility. We have taken ECIS measurements on several cultures of non-cancerous (HOSE) and cancerous (SKOV) human ovarian surface epithelial cells. By analyzing the noise in
arxiv   +1 more source

Rotating bio-reactor cell culture apparatus [PDF]

open access: yes, 1991
A bioreactor system is described in which a tubular housing contains an internal circularly disposed set of blade members and a central tubular filter all mounted for rotation about a common horizontal axis and each having independent rotational support ...
Schwarz, Ray P., Wolf, David A.
core   +1 more source

Studies of intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes (PMNS). I. Role of contact inhibition of locomotion. [PDF]

open access: yes, 1975
Intercellular invasion is the active migration of cells on one type into the interiors of tissues composed of cells of dissimilar cell types. Contact paralysis of locomotion is the cessation of forward extension of the pseudopods of a cell as a result of
Armstrong, Peter, Lackie, J
core   +1 more source

Elucidation of interface interactions between a dehydratase domain and an acyl carrier protein in cremimycin polyketide synthase

open access: yesFEBS Letters, EarlyView.
In modular polyketide synthases, the dehydratase (DH) domain catalyzes the dehydration reaction of the β‐hydroxyacyl unit attached to the cognate acyl carrier protein (ACP) domain. However, it is unclear how DH interacts with ACP during the reaction. In this study, we identified DH–ACP interface residues, providing the first detailed insights into DH ...
Kaede Kotagiri   +8 more
wiley   +1 more source

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