Results 111 to 120 of about 583,264 (306)

Viral contamination in cell culture: analyzing the impact of Epstein Barr virus and Ovine Herpesvirus 2

Frontiers in Microbiology
Cell culture techniques are increasingly favored over animal models due to rising costs, time constraints, and ethical concerns regarding animal use. These techniques serve critical roles in disease modeling, drug screening, drug discovery, and toxicity ...
Iman M. Bastawecy, Mohamed Abdelmonem, Ahmed F. Afify, Norazalina Saad, Yuki Shirosaki, Yuki Shirosaki, Yuki Shirosaki, Che Azurahanim Che Abdullah, Rania F. El Naggar, Mohammed A. Rohaim, Mohammed A. Rohaim, Muhammad Munir   +11 more
doaj   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Autologous chondrocyte implantation to treat femoral condyle and talar lesions [PDF]

Einstein (São Paulo), 2008
Objective: To show the first Brazilian autologous chondrocyteimplantation. Methods: Young patients with localized lesions infemoral condyle and talus were selected. The clinical evaluationwas complemented with the imaginologic resources.
Moises Cohen, Caio Nery, Maria Stella Peccin, Cibele Ramos Réssio, Inácio Diogo Asaumi, Christiane Bertachini Lombello   +5 more
doaj  

Inhibition of cyclin‐dependent kinases 12/13 using CT7439 as a treatment for colorectal cancer with CDK12 upregulation

Molecular Oncology, EarlyView.
The proposed mechanism of action for the CDK12/13 inhibitor and cyclin K degrader, CT7439. CDK12/13 inhibition interrupts transcription elongation, leading to increased DNA damage that results in cell death. This agent is a potentially novel treatment option for patients with colorectal cancer. Created in BioRender. Cyclin‐dependent kinase (CDK) 12 and
Wylie K. Watlington, Divya L. Dayanidhi, Mohammad Zokaasadi, John B. Mantyh, Pelumi D. Olawuni, Gabrielle Rupprecht, Jeremy M. Force, Shannon McCall, Ashwani K. Bahl, David S. Hsu, Jason A. Somarelli   +10 more
wiley   +1 more source

Evaluation of Monitoring Approaches and Effects of Culture Conditions On Recombinant Protein-production in Baculovirus-infected Insect Cells [PDF]

The baculovirus infection process of Spodoptera frugiperda (Sf9) insect cells in oxygen-controlled bioreactors in serum-free medium was investigated using a recombinant Autographa californica (AcNPV) virus expressing beta-galactosidase enzyme as a model ...
Hensler, WT., Cell Culture Engineering IV Conference, Agathos, Spiros N.   +2 more
core  

The effect of modulating membrane lipid composition on the thermal sensitivity of tumour cells in culture [PDF]

, 1989
The plasma membrane, which separates intracellular contents from extra cellular milieu, consists of a lipid bilayer comprising mainly phospholipids and cholesterol together with various functional proteins, which control the interaction of the cell with ...
Kingston, Catharine Alison
core  

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source

Development of tetraploids in tissue culture: modern techniques and biotechnological innovations [PDF]

Polyploidy, characterized by chromosome multiplication, plays a pivotal role in plant evolution and agricultural innovation. This review explores advanced methodologies for tetraploid development in tissue culture, highlighting chemical inducers such as ...
Mohamed, Heba I., Baloch, Faheem Shehzad, Soomro, Salma Naimatullha, Liaqat, Waqas, Nadeem, Muhammad Azhar, Altaf, Muhammad Tanveer, Soomro, Sabeen Rehman   +6 more
core   +1 more source

ZW4864‐mediated inhibition of the β‐catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer

Molecular Oncology, EarlyView.
BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi, Mandy Berndt‐Paetz, Eileen Lerner, Gregoire Najjar, Anca Azoitei, Krishna Pal Singh, Shailendra Kumar Gupta, Olaf Wolkenhauer, Cagatay Günes, Otmar Huber, Marc‐Oliver Grimm, Daniel Steinbach   +11 more
wiley   +1 more source