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Studies of renal cell function using cell culture techniques
American Journal of Physiology-Renal Physiology, 1980Cell culture, a powerful tool for the study of cell biology, offers advantages for the study of renal cell function. Epithelial cells derived from a variety of organs, including the kidney, form oriented epithelial sheets in culture that have many structural characteristics (microvilli, tight junctions) of epithelia in situ.
J S, Handler, F M, Perkins, J P, Johnson
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General Techniques of Cell Culture
1997Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a
Maureen A. Harrison, Ian F. Rae
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Techniques for Culturing Arterial Endothelial Cells
1977Lazzarini (3) was one of the first to make use of the methods of tissue culture in the study of the nature of cells of blood vessels. His pioneer work was continued and extended by Pollack and his group (5). Like any other new approach this also had been vigorously discussed until recently.
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Optimization techniques: Studies in cell culture
Drug Development and Industrial Pharmacy, 1983AbstractWith the present large worldwide demand for vaccines and cellular by products, a number of anchorage-dependent cell mass culture systems have been developed. To obtain the maximum yield of product per run, the number of cells per system volume must be maximized and yet maintain efficiency.
E. M. Scattergood +4 more
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Overview of Cell and Tissue Culture Techniques
Current Protocols in Pharmacology, 1999AbstractCulture preparations vary greatly in complexity, ranging from single isolated cells to three‐dimensional histotypic cell structures. Besides cultures obtained directly from animal tissues (primary cultures), permanent cultures of continuously dividing (immortalized) cells have been established.
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Basic Techniques in Mammalian Cell Tissue Culture
Current Protocols in Cell Biology, 2007AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media
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Culture Technique of Human Pituitary Adenoma Cells
2003Cell cultures are indispensable to the study of human pituitary adenomas Nevertheless, in vitro studies of human pituitary adenomas have been hindered by the small amount of tissue available and by difficulties in establishing monolayer cultures using standard culture techniques owing to poor cell attachment to ordinary plastic flasks It is well known ...
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