Nuclear DNA and protein content evaluation in Taxus plant cell cultures using multiparameter flow cytometry [PDF]
, 2009 Plant cell cultures of Taxus provide the most reliable production methods for the anti-cancer drug paclitaxel. In order to comprehend the inherent culture heterogeneity and production variability in cell cultures, it is essential that the cellular ...
Amit Shavit +2 more
core +2 more sources
Journal of Applied Oral Science, 2007 The objective of the present study was to characterize bone cells grown in two culture media, and to determine the effective concentration of OP-1 on the growth of osteo-1 cells.
Adriane Yaeko Togashi +4 more
doaj +1 more source
Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering [PDF]
, 2019 Industrial plant biotechnology applications include the production of sustainable fuels, complex metabolites and recombinant proteins, but process development can be impaired by a lack of reliable and scalable screening methods. Here, we describe a rapid
Arfi +28 more
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Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation [PDF]
, 1999 Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger faeces. Immunomagnetic capture, immunofluorescence and selective culture confirmed species identification and cell viability.
Sweeney, F. P. +6 more
core +4 more sources
Diel rhythmicity in amino acid uptake by Prochlorococcus [PDF]
, 2008 The marine cyanobacterium Prochlorococcus, the most abundant phototrophic organism on Earth, numerically dominates the phytoplankton in nitrogen (N)-depleted oceanic gyres.
Burkill, Peter H. +7 more
core +3 more sources
An Overview of in vitro Methods to Study Microglia
Frontiers in Cellular Neuroscience, 2018 Neuroinflammation is a common feature in neurodegenerative diseases and strategies to modulate neuroinflammatory processes are increasingly considered as therapeutic options.
Raissa Timmerman +2 more
doaj +1 more source
Automated Motion Analysis of Adherent Cells in Monolayer Culture [PDF]
, 2015 This paper presents a novel method for tracking and characterizing adherent cells in monolayer culture. A system of cell tracking employing computer vision techniques was applied to time-lapse videos of replicate normal human uro-epithelial cell cultures
Bedder, M. +5 more
core +1 more source
Modeling Central Nervous System Injury In Vitro: Current Status and Promising Future Strategies
Biomedicines, 2022 The central nervous system (CNS) injury, which occurs because of mechanical trauma or ischemia/hypoxia, is one of the main causes of mortality and morbidity in the modern society.
Kristina Pilipović +3 more
doaj +1 more source
Advanced imaging and tissue engineering of the human limbal epithelial stem cell niche [PDF]
, 2015 The limbal epithelial stem cell niche provides a unique, physically protective environment in which limbal epithelial stem cells reside in close proximity with accessory cell types and their secreted factors.
Daniels, JT +10 more
core +1 more source
Memantine protects the cultured rat hippocampal neurons treated by NMDA and amyloid β1–42
Frontiers in Neuroscience, 2023 Alzheimer’s disease (AD) is a devastating neurodegenerative condition with no effective treatments. Recent research highlights the role of NMDA receptors in AD development, as excessive activation of these receptors triggers excitotoxicity. Memantine, an
Nataliia M. Rozumna +2 more
doaj +1 more source