Results 31 to 40 of about 1,114,098 (265)

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

FEBS Letters, EarlyView.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring, Morten Roedgaard, Camilla Myrup Holst, Helene Christensen, Birgitta R. Knudsen, Lotte Bjergbaek, Anni Hangaard Andersen   +6 more
wiley   +1 more source

STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence

FEBS Letters, EarlyView.
Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek, Ariane Fischer, Mariano Ruz Jurado, Anita Tamiato, Tara Procida‐Kowalski, Jochen Wilhelm, Dennis Hecker, Maximilian Merten, Felicitas Escher, Badder Kattih, Valentina Puntmann, David John, Marcel H. Schulz, Eike Nagel, Stefanie Dimmeler, Guillermo Luxán   +15 more
wiley   +1 more source

Cellular Cardiomyoplasty: Clinical Application [PDF]

, 2004
Myocardial regeneration can be induced with the implantation of a variety of myogenic and angiogenic cell types. More than 150 patients have been treated with cellular cardiomyoplasty worldwide, 18 patients have been treated by our group.
Acar, C. (Christophe), Carpentier, A. (A.), Chachques, J. (J.), D’Attellis, N. (N.), Fabiani, J.N. (J.N.), Herreros, J. (Jesús), Prosper, F. (Felipe), Trainini, J. (Jorge)   +7 more
core   +1 more source

History of Cell Culture Techniques

The American Biology Teacher, 2010
Culturing Life: How Cells Became Technologies. By Hannah Landecker . 2007. Harvard University Press (ISBN 9780674034761). 276 pages. Paperback. $$18.95. Cell culture techniques have shaped the scope of biology since the start of the 20th century and are responsible for major advances in biomedicine.
openaire   +2 more sources

Protonophore activity of short‐chain fatty acids induces their intracellular accumulation and acidification

FEBS Letters, EarlyView.
The protonated form of butyrate, as well as other short‐chain fatty acids (SCFAs), is membrane permeable. In acidic extracellular environments, this can lead to intracellular accumulation of SCFAs and cytosolic acidification. This phenomenon will be particularly relevant in acidic environments such as the large intestine or tumor microenvironments ...
Muwei Jiang, Frans Bianchi, Geert van den Bogaart   +2 more
wiley   +1 more source

Advanced imaging and tissue engineering of the human limbal epithelial stem cell niche [PDF]

, 2015
The limbal epithelial stem cell niche provides a unique, physically protective environment in which limbal epithelial stem cells reside in close proximity with accessory cell types and their secreted factors.
Daniels, JT, Dziasko, M, Funderburgh, JL, Kureshi, A, Levis, HJ, Massie, I, Morgan, L, Neale, M, Sheth, R, Tovell, VE, Vernon, AJ   +10 more
core   +1 more source

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

One‐dimensional patterning of cells in silicone wells via compression‐induced fracture [PDF]

, 2014
We have adapted our existing compression‐induced fracture technology to cell culture studies by generating linear patterns on a complex cell culture well structure rather than on simple solid constructs.
Baker, Chen, Cukierman, Cukierman, Curtis, Dahl, Doyle, Flemming, Flemming, Green, Harunaga, Hoffman-Kim, Huebsch, Justice, Kilian, Kim, Kim, Kooten, Kraehenbuehl, Kraning-Rush, Kriparamanan, Kulangara, Lee, Levina, Li, Martínez, McBeath, Mills, Moraes, Moraes, Moraes, Nathan, Pampaloni, Picone, Raghavan, Smalley, ten Elshof, Thouless, Théry, Treiser, Uchida, Vignaud, Xia, Yamada, Yim, Zhou, Zhu   +46 more
core   +1 more source

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source