Results 71 to 80 of about 583,264 (306)
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Lab-in-a-trench platform for real-time monitoring of cell surface protein expression [PDF]
, 2010 In this work we for the first time demonstrate real-time monitoring of the expression of membrane proteins in native, live cells, free of hydrodynamic stress at single cell resolution. This micro-optofluidic mechanism is uniquely enabled by the intricate
Dimov, Ivan Krastev +6 more
core +1 more source
The importance of determining lactate dehydrogenase in laboratory and experimental work in oncology [PDF]
Vojnosanitetski Preglednema
Jurišić Vladimir +3 more
doaj +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Synchronized Mammalian Cells in Culture: A Comparison of Normal and KB Cancer Cell Growth [PDF]
, 2012 Experimentation and tests with cells in culture and cell synchronization or synchrony are important in order to be able to make both reliable and high-sensitivity measurements. This is also very important for mechanistic studies of cell division control,
I. C. Baianu
core +1 more source
3D cell culture models of epithelial tissues [PDF]
, 2019 Cells in tissues in vivo face a very different microenvironment than typical cultured cells plated on a plastic dish. Already several decades ago, cell biologists observed that cell lines show dramatically different morphology and growth characteristics ...
Zhang, K. (Kai) +5 more
core +1 more source
FEBS Letters, EarlyView.Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani +3 more
wiley +1 more source
THREE-DIMENSIONAL MICROFLUIDIC CELL CULTURE OF STEM CELL-DERIVED NEURONAL MODELS OF PARKINSON'S DISEASE [PDF]
, 2016 Cell culture models in 3D have become an essential tool for the implementation of cellular models of neurodegenerative diseases. Parkinson’s disease (PD) is characterized by the loss of dopaminergic neurons from the substantia nigra.
LUCUMI-MORENO, Edinson
core
Identification of the plant mitochondrial OrfX protein: A mass spectrometry approach
FEBS Letters, EarlyView.The mitochondrial genome of plants contains an open reading frame, orfx, which encodes a rare protein that has so far escaped mass spectrometric detection. The protein resembles the c‐subunit of bacterial twin‐arginine‐motif‐dependent protein translocases (TatC).
Matthias Döring +3 more
wiley +1 more source