Results 101 to 110 of about 8,789,133 (312)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

The terminal basal mitosis of chicken retinal Lim1 horizontal cells is not sensitive to cisplatin-induced cell cycle arrest

, 2014
For proper development, cells need to coordinate proliferation and cell cycle-exit. This is mediated by a cascade of proteins making sure that each phase of the cell cycle is controlled before the initiation of the next.
Thyselius, Malin, Hallböök, Finn, Shirazi Fard, Shahrzad,, Hallböök, Finn,, Shirazi Fard, Shahrzad, All-Ericsson, Charlotta, Thyselius, Malin,   +6 more
core   +1 more source

Phosphatase POPX2 interferes with cell cycle by interacting with Chk1

, 2020
Protein-protein interaction network analysis plays critical roles in predicting the functions of target proteins. In this study, we used a combination of SILAC-MS proteomics and bioinformatic approaches to identify Checkpoint Kinase 1 (Chk1) as a ...
Kim, Pu Rum, Lee, Raphael Tze Chuen, Koon, Yen Ling, Koh, Dylan Hong Zheng, Chiam, Keng-Hwee, Koh, Cheng-Gee, Azizan, Farouq   +6 more
core   +1 more source

The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion [PDF]

, 2000
Duplicating centrosomes are paired during interphase, but are separated at the onset of mitosis. Although the mechanisms controlling centrosome cohesion and separation are important for centrosome function throughout the cell cycle, they remain poorly ...
Fry, A. M., Erich A. Nigg, Andrew M. Fry, Stierhof, Y. D., Mayor, T., York-Dieter Stierhof, Tanaka, K., Nigg, E. A., Thibault Mayor, Kayoko Tanaka, Nigg EA, Fry AM, Tanaka K, Mayor T, Stierhof YD   +14 more
core   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Cell cycle regulated expression of the WHI7 Start repressor gene [PDF]

Periodic transcriptional waves along the cell cycle ensure the accurate progression of the different cell cycle phases through the timely regulated expression of cell cycle proteins.
Ros Carrero, Cristina, Gomar Alba, Mercè, Igual García, Juan Carlos   +2 more
core   +1 more source

First genome-wide centromere map of Trypanosoma cruzi suggests linear and 3D compartment boundaries and spatial clustering

BMC Genomics
Background Trypanosoma cruzi, the etiological agent of Chagas disease, possesses a highly repetitive genome that has historically hindered high-quality assembly and structural characterization. Despite significant advances in assembling T. cruzi genomes,
Natália Karla Bellini, Pedro Gabriel Nachtigall, Pedro Leonardo Carvalho de Lima, Herbert Guimarães de Sousa Silva, David da Silva Pires, Vinicius Carius de Souza, Claudia Rabuffo, Danielle Bruno de Carvalho, Yasmin Morais Feno, Ana Paula Cabral de Araujo Lima, T. Nicolai Siegel, Julia Pinheiro Chagas da Cunha   +11 more
doaj   +1 more source

Phosphatase-regulated recruitment of the spindle- and kinetochore-associated (Ska) complex to kinetochores

Biology Open, 2017
Kinetochores move chromosomes on dynamic spindle microtubules and regulate signaling of the spindle checkpoint. The spindle- and kinetochore-associated (Ska) complex, a hexamer composed of two copies of Ska1, Ska2 and Ska3, has been implicated in both ...
Sushama Sivakumar, Gary J. Gorbsky
doaj   +1 more source