Results 131 to 140 of about 20,962,508 (350)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Ehbp1 orchestrates orderly sorting of Wnt/Wingless to the basolateral and apical cell membranes

EMBO Reports
Wingless (Wg)/Wnt signaling plays a critical role in both development and adult tissue homeostasis. In the Drosophila larval wing disc epithelium, the orderly delivery of Wg/Wnt to the apical and basal cell surfaces is essential for wing development ...
Yuan Gao, Jing Feng, Yansong Zhang, Mengyuan Yi, Lebing Zhang, Yan Yan, Alan Jian Zhu, Min Liu   +7 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

The effect of in vitro culture on the stability, expansion and neuronal differentiation of human pluripotent cell lines [PDF]

, 2010
Pluripotent cells are defined by their ability to both self-renew and to differentiation into any cell type within the human body. As such, pluripotent cell lines are of great interest as starting material for drug screening and cell therapies for ...
Gillett, M L, Gillett, M.L.
core  

Temporospatial induction of homeodomain gene cut dictates natural lineage reprogramming

eLife, 2018
Understanding how cellular identity naturally interconverts with high efficiency and temporospatial precision is crucial for regenerative medicine. Here, we revealed a natural midgut-to-renal lineage conversion event during Drosophila metamorphosis and ...
Ke Xu, Xiaodan Liu, Yuchun Wang, Chouin Wong, Yan Song   +4 more
doaj   +1 more source

Comparative analysis and refinement of human PSC-derived kidney organoid differentiation with single cell transcriptomics

Cell Stem Cell, 2018
Summary Kidney organoids derived from human pluripotent stem cells have great utility for investigating organogenesis and disease mechanisms, and potentially as a replacement tissue source, but how closely organoids derived from current protocols ...
Hao-Jia Wu, Kohei Uchimura, Erinn L. Donnelly, Yuhei Kirita, Samantha A. Morris, B. Humphreys   +5 more
semanticscholar   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Genome editing iPSC to purposing enhancement of induce CD8 killer T cell function for regenerative immunotherapy

Inflammation and Regeneration
In recent years, immunotherapy has become a standard cancer therapy, joining surgery, chemotherapy, and radiation therapy. This therapeutic approach involves the use of patient-derived antigen-specific T cells or genetically modified T cells engineered ...
Sota Kurihara, Akihiro Ishikawa, Shin Kaneko   +2 more
doaj   +1 more source