Results 31 to 40 of about 10,566 (169)

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Spontaneous whole genome duplication renders mouse embryonic fibroblasts resistant to reprogramming

Cell & Bioscience
Whole-genome duplication (WGD) is thought to be quite rare, but has important implications ranging from emergences of new species to tumorigenesis. The burden of doubled genome for a cell must be traumatic and remains poorly understood.
Wei Li, Lingyu Zhong, Pengli Li, Ziwei Zhai, Runxia Lin, Minjing Ke, Yixin Fan, Yu Liu, Yu Fu, Yue Qin, Chengchen Zhao, Bo Wang, Junqi Kuang, Duanqing Pei   +13 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

In vitro mimicking of humanized cardiogenesis under porcine condition

Cell & Bioscience
Background One promising strategy to generate humanized organs is through embryo complementation by injecting human pluripotent stem cells (PSCs) into gene-edited porcine embryos.
Jiajun Li, Changjiang Xu, Bingbo Shi, Yawen Lyu, Zejie Lin, Wei Shi, Jiachen Xu, Xinle Zou, Xiaomin Wang, Hanqing Zhao, Chengchen Zhao, Duanqing Pei   +11 more
doaj   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

Engineering mouse cell fate controller by rational design

Nature Communications
Cell fate is likely regulated by a common machinery, while components of this machine remain to be identified. Here we report the design and testing of engineered cell fate controller NanogBiD, fusing BiD or BRG1 interacting domain of SS18 with Nanog ...
Tao Huang, Dong Liu, Xiaomin Wang, Junqi Kuang, Manqi Wu, Beibei Wang, Zechuan Liang, Yixin Fan, Bo Chen, Zhaoyi Ma, Yu Fu, Wenhui Zhang, Jin Ming, Yue Qin, Chengchen Zhao, Bo Wang, Duanqing Pei   +16 more
doaj   +1 more source

Ki67 Immunofluorescence on Bovine Cell Lines

Bio-Protocol, 2013
This is a rapid protocol to test the effects of drugs treatment on bovine cell replication using Ki67 staining. Ki67 is associated with cell proliferation and is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent ...
Justine Marsolier, Souhila Medjkane, Martine Perichon, Jonathan Weitzman   +3 more
doaj   +1 more source

NeST: nested hierarchical structure identification in spatial transcriptomic data

Nature Communications, 2023
Spatial gene expression in tissue is characterized by regions in which particular genes are enriched or depleted. Frequently, these regions contain nested inside them subregions with distinct expression patterns.
Benjamin L. Walker, Qing Nie
doaj   +1 more source

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

Transcriptional profiling of circulating extracellular vesicles from prebiopsy prostate cancer patients

Molecular Oncology, EarlyView.
RNA profiling of circulating extracellular vesicles (EVs) from blood samples of men undergoing prostate biopsy identifies transcripts associated with clinically significant prostate cancer. Integrative analysis with public tumor datasets links EV‐derived gene signatures to tumor stage and progression‐free survival, highlighting CASP3, XRCC2, and RIT1 ...
Stefan Werner, Pierre Tennstedt, Randi C. Pose, Christian Müller, Katharina Besler, Svenja Schneegans, Malik Alawi, Marie C. Roesch, Sven Peine, Desiree Bonci, Joanna Budna‐Tukan, Evi Lianidou, Catherine Alix‐Panabières, Derya Tilki, Klaus Pantel   +14 more
wiley   +1 more source