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Retinal Ganglion Cell Axon Fractionation
2023Retinal ganglion cell (RGC) axon regeneration in mammals can be stimulated through gene knockouts, pharmacological agents, and biophysical stimulation. Here we present a fractionation method to isolate regenerating RGC axons for downstream analysis using immunomagnetic separation of cholera toxin subunit B (CTB)-bound RGC axons.
Sean D, Meehan, Sanjoy, Bhattacharya
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Cellular Fractionation – Yeast Cells
2013In proteomics research, one essential step among enrichment techniques is subcellular fractionation. This is of special importance for analyzing intracellular organelles and multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach to reducing sample complexity and is most efficiently combined with high-resolution 2-D gel ...
Joerg, von Hagen, Uwe, Michelsen
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Cellular Fractionation – Mammalian Cells
2013In proteomics research, one essential step among enrichment techniques is subcellular fractionation. This is of special importance for analyzing intracellular organelles and multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach to reducing sample complexity and is most efficiently combined with high-resolution 2-D gel ...
Joerg, von Hagen, Uwe, Michelsen
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Overview of Cell Fractionation
Current Protocols in Protein Science, 1995AbstractThis discussion unit describes the most common methods for cell fractionation which provides the essential ingredients for the increasing number of cell‐free assays now being used in test‐tube reconstructions of complex cellular events involving intercompartmental interactions.
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Listeria Cell Wall Fraction: A B Cell Adjuvant
The Journal of Immunology, 1976Abstract A crude cell wall-rich fraction of Listeria monocytogenes (LCWF) has previously been shown to induce resistance to Listeria infection in mice, to be a murine B cell mitogen, and to be an immunologic adjuvant. Data reported here show that LCWF stimulates immune responses by three different murine B cell populations that respond ...
Campbell, P A +2 more
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BIOCHEMISTRY OF CELL FRACTIONS
Canadian Journal of Biochemistry and Physiology, 1957Null
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1976
Since the development of the first quantitative technique for fractionating liver tissue by Albert Claude (1946a,b), successive improvements have been introduced by several workers that have led to reproducible methods for disaggregating the tissue into a suspension of subcellular components,* called tissue homogenate, and for resolving this homogenate
Henri Beaufay, Alain Amar-Costesec
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Since the development of the first quantitative technique for fractionating liver tissue by Albert Claude (1946a,b), successive improvements have been introduced by several workers that have led to reproducible methods for disaggregating the tissue into a suspension of subcellular components,* called tissue homogenate, and for resolving this homogenate
Henri Beaufay, Alain Amar-Costesec
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Cell Fractionation of Parasitic Protozoa
2008Cell fractionation, a methodological strategy for obtaining purified organelle preparations, has been applied successfully to parasitic protozoa by a number of researchers. These studies have provided new information of the cell biology of these parasites and have supported investigators to assume that some of the protozoa form the roots of the ...
Wanderley, de Souza +2 more
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Analytical fractionation of cultured hepatoma cells (HTC cells)
Biochimica et Biophysica Acta (BBA) - General Subjects, 1978Homogenates of HTC cells have been fractionated by differential centrifugation (in four particulate fractions: N, M, L, P, and a supernatant S) or isopycnic banding in linear sucrose gradients. On this basis, the following subcellular organelles may be characterized: (i) Mitochondria, detected by cytochrome oxidase and succinodehydrogenase, are ...
P, Lopez-Saura, A, Trouet, P, Tulkens
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Biochemical Fractionation of Drosophila Cells
2007This chapter describes how to perform basic biochemical fractionations of Drosophila cells, and how to begin to characterize the proteins in the resulting fractions. The protocols include maintenance and transfection of Drosophila cell lines (Section 3.1.), hypotonic lysis (Section 3.2.), and separation of cellular lysates into cytosolic and membrane ...
Melanie, Stegman, David, Robbins
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