Results 101 to 110 of about 5,163,880 (297)
FEBS Letters, EarlyView.Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source
STAR ProtocolsSummary: Extracellular vesicles (EVs) mediate intercellular communication by transferring bioactive molecular cargo. We present a protocol for isolating EVs from fibroblast growth factor-2 (FGF2)-primed primary mouse astrocytes using differential ...
Xiaomin Wen +5 more
doaj +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
The fractionation of lymphoid cells
, 1972 The work described in this thesis set out to elucidate the heterogeneity of lymphocytes from rats by investigating methods for the separation and analysis of lymphocyte sub-populations. In order to separate lymphocytes on the basis of differences in their size, the technique of velocity sedimentation under the influence of gravity in a shallow albumin ...
Hunt, S, Hunt, S. V.
openaire +2 more sources
FEBS Letters, EarlyView.How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley +1 more source
STAR ProtocolsSummary: Modifications to the plasma membrane proteome reflect neuronal physiological states. Here, we present a protocol to profile surface proteins from mouse cortical neuron cultures using in situ biotinylation.
Liang Shi +4 more
doaj +1 more source
On-farm fractionation of milk components [PDF]
, 2006 Methods for on-farm extraction of low-concentration (minor) proteins from raw whole bovine milk directly after milking were explored. These minor proteins have high commercial value.
Chand, Amita
core
Effects of Fractionation three on the cell wall and cell membrane permeability.
, 2014 Effect of Fractionation three on the alkaline phosphatase (AKP) activity of C. michiganense subsp. sepedonicum (A). Effect of Fractionation three on the leakage of nucleotides from C. michiganense subsp. sepedonicum (B). Cell membrane depolarization in C.
Jin Cai (430624) +4 more
core +1 more source
Cell Competition and its role in the regulation of cell fitness from development to cancer [PDF]
, 2016 Cell competition is a cell fitness-sensing mechanism conserved from insects to mammals that eliminates those cells that, although viable, are less fit than their neighbors. An important implication of cell competition is that cellular fitness is not only
di gregorio, A, Rodriguez, T, Bowling, S
core +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source