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Cellular Fractionation – Yeast Cells
2013In proteomics research, one essential step among enrichment techniques is subcellular fractionation. This is of special importance for analyzing intracellular organelles and multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach to reducing sample complexity and is most efficiently combined with high-resolution 2-D gel ...
Joerg, von Hagen, Uwe, Michelsen
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Cell Fractionation of Parasitic Protozoa
2008Cell fractionation, a methodological strategy for obtaining purified organelle preparations, has been applied successfully to parasitic protozoa by a number of researchers. These studies have provided new information of the cell biology of these parasites and have supported investigators to assume that some of the protozoa form the roots of the ...
Wanderley, de Souza +2 more
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Trends in Biochemical Sciences, 1980
Simple, effective and inexpensive methods for cell separation by selective agglutination with lectins or on columns of immobilized lectins have been developed. They are gaining popularity for the fractionation of murine and human lymphocytes into biologically distinct subpopulations.
Yair Reisner, Nathan Sharon
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Simple, effective and inexpensive methods for cell separation by selective agglutination with lectins or on columns of immobilized lectins have been developed. They are gaining popularity for the fractionation of murine and human lymphocytes into biologically distinct subpopulations.
Yair Reisner, Nathan Sharon
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Biochemical Fractionation of Drosophila Cells
2007This chapter describes how to perform basic biochemical fractionations of Drosophila cells, and how to begin to characterize the proteins in the resulting fractions. The protocols include maintenance and transfection of Drosophila cell lines (Section 3.1.), hypotonic lysis (Section 3.2.), and separation of cellular lysates into cytosolic and membrane ...
Melanie, Stegman, David, Robbins
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Cellular Fractionation – Mammalian Cells
2013In proteomics research, one essential step among enrichment techniques is subcellular fractionation. This is of special importance for analyzing intracellular organelles and multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach to reducing sample complexity and is most efficiently combined with high-resolution 2-D gel ...
Joerg, von Hagen, Uwe, Michelsen
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Specific Fractionation of Immunocompetent Cells
Immunological Reviews, 1970Prominent progress in molecular biology was only made when it became possible to separate functionally distinct molecules by taking advantage of their biophysical properties. Likewise, the analysis of the functions of heter ogeneous populations of immunocompetent cells, as to the functional properties of their various subpopulations, can not be done ...
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Lipolytic activity in adipocyte cell fractions
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980Adipocytes release only negligible amounts of free fatty acids unless stimulated, but reveal considerable lipolytic activity when homogenized. Epinephrine treatment of the cells caused only a 20-40% increase in the activity of infranatants of homogenates while raising the activity associated with the fat layer up to 10-fold. Full activity (i.e. that of
Y, Oschry, B, Shapiro
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Nonaqueous fractionation of HeLa cells in glycols
Analytical Biochemistry, 1984A further modification of Behrens ' method of nonaqueous cell fractionation, using glycols as media for homogenization and centrifugation, was presented. HeLa cells were frozen in melting Freon-12 ( CCl2F2 ), dried under vacuum at -30 degrees C, sonicated in hexylene glycol at -35 degrees C, and centrifuged through either propylene glycol or a mixture ...
T, Gurney, M W, Collard
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Differential Detergent Fractionation of Eukaryotic Cells
Cold Spring Harbor Protocols, 2005INTRODUCTIONDifferential detergent fractionation (DDF) involves the sequential extraction of cells with PIPES buffers containing first digitonin, then Triton, and finally Tween/deoxycholate (DOC). The procedure yields four biochemically and electrophoretically distinct fractions composed of the following: (1) cytosolic proteins and extractable ...
Melinda, Ramsby, Gregory, Makowski
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1976
Since the development of the first quantitative technique for fractionating liver tissue by Albert Claude (1946a,b), successive improvements have been introduced by several workers that have led to reproducible methods for disaggregating the tissue into a suspension of subcellular components,* called tissue homogenate, and for resolving this homogenate
Henri Beaufay, Alain Amar-Costesec
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Since the development of the first quantitative technique for fractionating liver tissue by Albert Claude (1946a,b), successive improvements have been introduced by several workers that have led to reproducible methods for disaggregating the tissue into a suspension of subcellular components,* called tissue homogenate, and for resolving this homogenate
Henri Beaufay, Alain Amar-Costesec
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