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Methods in molecular biology, 2017 Protein function is generally dependent on its subcellular localisation. In Gram-negative bacteria such as Escherichia coli, a protein can be targeted to five different compartments: the cytoplasm, the inner membrane, the periplasm, the outer membrane and the extracellular medium.
Mélissa Petiti +2 more
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Methods in molecular biology, 2014
Proteins within a cell are localized into specific cellular compartments, allowing compartmentalization of distinct tasks. If we consider lipid bilayers as compartments, then gram-negative bacteria such as Pseudomonas aeruginosa can target proteins to five distinct locations: the cytoplasm, the inner membrane, the periplasm, the outer membrane, and the
B. Ize, Véronique Viarre, R. Voulhoux
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Proteins within a cell are localized into specific cellular compartments, allowing compartmentalization of distinct tasks. If we consider lipid bilayers as compartments, then gram-negative bacteria such as Pseudomonas aeruginosa can target proteins to five distinct locations: the cytoplasm, the inner membrane, the periplasm, the outer membrane, and the
B. Ize, Véronique Viarre, R. Voulhoux
semanticscholar +5 more sources
Overview of Cell Fractionation
Current Protocols in Protein Science, 1995AbstractThis discussion unit describes the most common methods for cell fractionation which provides the essential ingredients for the increasing number of cell‐free assays now being used in test‐tube reconstructions of complex cellular events involving intercompartmental interactions.
J. Castle
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, 1976
Since the development of the first quantitative technique for fractionating liver tissue by Albert Claude (1946a,b), successive improvements have been introduced by several workers that have led to reproducible methods for disaggregating the tissue into a suspension of subcellular components,* called tissue homogenate, and for resolving this homogenate
H. Beaufay, A. Amar‐Costesec
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Since the development of the first quantitative technique for fractionating liver tissue by Albert Claude (1946a,b), successive improvements have been introduced by several workers that have led to reproducible methods for disaggregating the tissue into a suspension of subcellular components,* called tissue homogenate, and for resolving this homogenate
H. Beaufay, A. Amar‐Costesec
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Retinal Ganglion Cell Axon Fractionation
2023Retinal ganglion cell (RGC) axon regeneration in mammals can be stimulated through gene knockouts, pharmacological agents, and biophysical stimulation. Here we present a fractionation method to isolate regenerating RGC axons for downstream analysis using immunomagnetic separation of cholera toxin subunit B (CTB)-bound RGC axons.
Sean D, Meehan, Sanjoy, Bhattacharya
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Trends in Biochemical Sciences, 1980
Simple, effective and inexpensive methods for cell separation by selective agglutination with lectins or on columns of immobilized lectins have been developed. They are gaining popularity for the fractionation of murine and human lymphocytes into biologically distinct subpopulations.
Y. Reisner, N. Sharon
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Simple, effective and inexpensive methods for cell separation by selective agglutination with lectins or on columns of immobilized lectins have been developed. They are gaining popularity for the fractionation of murine and human lymphocytes into biologically distinct subpopulations.
Y. Reisner, N. Sharon
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Cellular Fractionation – Yeast Cells
2013In proteomics research, one essential step among enrichment techniques is subcellular fractionation. This is of special importance for analyzing intracellular organelles and multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach to reducing sample complexity and is most efficiently combined with high-resolution 2-D gel ...
Joerg, von Hagen, Uwe, Michelsen
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Cellular Fractionation – Mammalian Cells
2013In proteomics research, one essential step among enrichment techniques is subcellular fractionation. This is of special importance for analyzing intracellular organelles and multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach to reducing sample complexity and is most efficiently combined with high-resolution 2-D gel ...
Joerg, von Hagen, Uwe, Michelsen
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Listeria Cell Wall Fraction: A B Cell Adjuvant
The Journal of Immunology, 1976Abstract A crude cell wall-rich fraction of Listeria monocytogenes (LCWF) has previously been shown to induce resistance to Listeria infection in mice, to be a murine B cell mitogen, and to be an immunologic adjuvant. Data reported here show that LCWF stimulates immune responses by three different murine B cell populations that respond ...
Campbell, P A +2 more
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