Experimental setup for the ultrasonic fractionation of flowing whole blood in a capillary
Current Directions in Biomedical Engineering, 2022 If a long ultrasound driving pulse is chosen such, that its frequency is greater than the resonance frequency of one cell type and less than the resonance frequency of another cell type, the cells are separated in different nodes and antinodes of the ...
Smalberger Charl +6 more
doaj +1 more source
Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway [PDF]
, 2012 Protein phosphatases play a key role in balancing the cellular responses to the transforming growth factor-beta (TGF beta) signals. Several protein phosphatases have been attributed roles in the regulation of the TGF beta pathway. Among these.
Bruce, David L. +4 more
core +3 more sources
FEBS Letters, EarlyView.The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li +2 more
wiley +1 more source
Positionally dependent ^(15)N fraction factors in the UV photolysis of N_2O determined by high resolution FTIR spectroscopy [PDF]
, 2000 Positionally dependent fractionation factors for the photolysis of isotopomers of N_2O in natural abundance have been determined by high resolution FTIR spectroscopy at three photolysis wavelengths.
Blake, G. A. +6 more
core
Geochemical support for a climbing habit within the Paleozoic seed fern genus Medullosa [PDF]
, 2011 A long-standing problem in paleobotany is the accurate identification of the growth habits and statures of fossil plants. Tissue-specific analysis of stable carbon isotope ratios in plant fossils can provide an independent perspective on this issue ...
Fischer, Woodward W. +1 more
core +2 more sources
Cell wall target fragment discovery using a low‐cost, minimal fragment library
FEBS Letters, EarlyView.LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan +5 more
wiley +1 more source
Hypofractionated versus standard fractionation radiotherapy for merkel cell carcinoma
Radiation OncologyPurpose/Objective(s) Merkel cell carcinoma (MCC) radiation treatment has historically consisted of standard 1.8–2 Gy fractions treated daily over 4–6 weeks.
Lorein Gonzalez +4 more
doaj +1 more source
Protocol for the purification and analysis of nuclear UFMylated proteins
STAR ProtocolsSummary: Protein UFMylation regulates numerous cellular processes including ribosome quality control and nuclear DNA repair. Here, we present a technique to isolate nuclei and purify UFMylated proteins under denaturing non-reducing conditions from ...
Pudchalaluck Panichnantakul +1 more
doaj +1 more source
The Amino Terminus of the Yeast F_1-ATPase β-Subunit Precursor Functions as a Mitochondrial Import Signal [PDF]
, 1986 The ATP2 gene of Saccharomyces cerevisiae codes for the cytoplasmically synthesized beta-subunit protein of the mitochondrial F1-ATPase. To define the amino acid sequence determinants necessary for the in vivo targeting and import of this protein into ...
Douglas, Michael G. +5 more
core
Attempts to detect retrotransposition and de novo deletion of Alus and other dispersed repeats at specific loci in the human genome [PDF]
, 2001 Dispersed repeat elements contribute to genome instability by de novo insertion and unequal recombination between repeats. To study the dynamics of these processes, we have developed single DNA molecule approaches to detect de novo insertions at a single
AJ Jeffreys +14 more
core +1 more source