Results 71 to 80 of about 5,651,556 (349)

Long-term cultivation of two diploid epithelial cell lines derived from normal rat liver cells [PDF]

, 1974
To obtain a useful rat liver cell line for in vitro carcinogenesis, two rat diploid epithelial cell lines were established from a 7-day-old male rat by the repeated colonial clone method.
Masuji, Hiroshi, Nakabayashi, Hidekazu, Sato, Jiro   +2 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients

Stem Cells International, 2019
Induced pluripotent stem cells (iPSCs) from type 1 long QT (LQT1) patients can differentiate into cardiomyocytes (CMs) including ventricular cells to recapitulate the disease phenotype. Although optical recordings using membrane potential dyes to monitor
Tadashi Takaki, Azusa Inagaki, Kazuhisa Chonabayashi, Keiji Inoue, Kenji Miki, Seiko Ohno, Takeru Makiyama, Minoru Horie, Yoshinori Yoshida   +8 more
doaj   +1 more source

Positioning of nucleosomes containing γ-H2AX precedes active DNA demethylation and transcription initiation

Nature Communications, 2021
The order of DNA methylation and histone modifications during transcription remained unclear. Here the authors show that HMGA2 induces DNA nicks at TGFB1-responsive genes, promoting nucleosome incorporation containing γ-H2AX, which is required for repair-
Stephanie Dobersch, Karla Rubio, Indrabahadur Singh, Stefan Günther, Johannes Graumann, Julio Cordero, Rafael Castillo-Negrete, Minh Bao Huynh, Aditi Mehta, Peter Braubach, Hector Cabrera-Fuentes, Jürgen Bernhagen, Cho-Ming Chao, Saverio Bellusci, Andreas Günther, Klaus T. Preissner, Sita Kugel, Gergana Dobreva, Malgorzata Wygrecka, Thomas Braun, Dulce Papy-Garcia, Guillermo Barreto   +21 more
doaj   +1 more source

Leptin Enhances Cholangiocarcinoma Cell Growth [PDF]

Cancer Research, 2008
Abstract Cholangiocarcinoma is a strongly aggressive malignancy with a very poor prognosis. Effective therapeutic strategies are lacking because molecular mechanisms regulating cholangiocarcinoma cell growth are unknown. Furthermore, experimental in vivo animal models useful to study the pathophysiologic mechanisms of malignant ...
G. Fava, G. Alpini, C. Rychlicki, S. Saccomanno, S. Demorrow, L. Trozzi, C. Candelaresi, J. Venter, A. Di Sario, M. Marzioni, I. Bearzi, S. Glaser, ALVARO, Domenico, L. Marucci, H. Francis, G. Svegliati Baroni, A. Benedetti   +16 more
openaire   +2 more sources

Xanthohumol, a Prenylated Chalcone Derived from Hops, Inhibits Growth and Metastasis of Melanoma Cells [PDF]

, 2021
Tatjana Seitz, Christina Hackl, K Freese, Peter Dietrich, A Mahli, Reinhard Thasler, Wolfgang E. Thasler, Sven Arke Lang, Anja‐Katrin Bosserhoff, Claus Hellerbrand   +9 more
openalex   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source

Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells

Cell Reports, 2017
Summary: Hepatocellular carcinoma (HCC) is an aggressive primary liver cancer. However, its origin remains a debated question. Using human data and various hepatocarcinogenesis mouse models, we show that, in early stages, transformed hepatocytes ...
Krishna S. Tummala, Marta Brandt, Ana Teijeiro, Osvaldo Graña, Robert F. Schwabe, Cristian Perna, Nabil Djouder   +6 more
doaj   +1 more source

Characterization and reduction of non-endocrine cells accompanying islet-like endocrine cells differentiated from human iPSC

Scientific Reports, 2022
The differentiation of pancreatic endocrine cells from human pluripotent stem cells has been thoroughly investigated for their application in cell therapy against diabetes.
Hideyuki Hiyoshi, Kensuke Sakuma, Noriko Tsubooka-Yamazoe, Shinya Asano, Taisuke Mochida, Junji Yamaura, Shuhei Konagaya, Ryo Fujii, Hirokazu Matsumoto, Ryo Ito, Taro Toyoda   +10 more
doaj   +1 more source