Results 61 to 70 of about 2,877,371 (319)

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Cell-free protein synthesis of membrane (1,3)-beta-D-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs [PDF]

, 2013
A membrane-embedded curdlan synthase (CrdS) from Agrobacterium is believed to catalyse a repetitive addition of glucosyl residues from UDP-glucose to produce the (1,3)-β-d-glucan (curdlan) polymer.
Mertens, Haydyn D.T., Garajová, Soňa, Hrmova, Maria, Garajova, S., Hrmova, M., Mertens, H., Waters, Shane, Amalraj, Amritha, Nagarajan, Yagnesh, Nagarajan, Y., Waters, S., Periasamy, Agalya, Shadiac, N., Periasamy, A., Shadiac, Nadim, Amalraj, A.   +15 more
core   +1 more source

Cytokinetic abscission requires actin-dependent microtubule severing

Nature Communications
Cell division is completed by the abscission of the intercellular bridge connecting the daughter cells. Abscission requires the polymerization of an ESCRT-III cone close to the midbody to both recruit the microtubule severing enzyme spastin and scission ...
Tamara Advedissian, Stéphane Frémont, Arnaud Echard   +2 more
doaj   +1 more source

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole

Nature Communications, 2018
Ciliogenesis is negatively regulated by the CP110-CEP97 complex, although the mechanism controlling mother centriole localization is poorly understood. Here, Huang et al.
Ning Huang, Donghui Zhang, Fangyuan Li, Peiyuan Chai, Song Wang, Junlin Teng, Jianguo Chen   +6 more
doaj   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Evaluation of membranes for use in on-line cell separation during mammalian cell perfusion processes

, 1994
Büntemeyer H, Bohme C, Lehmann J. Evaluation of membranes for use in on-line cell separation during mammalian cell perfusion processes. Cytotechnology. 1994;15(1-3):243-251.In this study two microporous hollow fibre membranes were evaluated for their use
Lehmann, Jürgen, Bohme, Christoph, Büntemeyer, Heino   +2 more
core   +1 more source

Chemotaxis: a feedback-based computational model robustly predicts multiple aspects of real cell behaviour [PDF]

, 2011
The mechanism of eukaryotic chemotaxis remains unclear despite intensive study. The most frequently described mechanism acts through attractants causing actin polymerization, in turn leading to pseudopod formation and cell movement.
Haastert, Peter J.M. van,, Peter J M van Haastert, van Haastert, Peter J. M., van Haastert, Peter J.M., Matthew P. Neilson, Douwe M Veltman, Webb, Steven D., Insall, Robert H., Webb, Steven D.,, Neilson, Matthew P.,, Neilson, Matthew P., Matthew P Neilson, Douwe M. Veltman, Peter J. M. van Haastert, Insall, Robert H.,, Steven D Webb, Webb, Steven, Robert H Insall, John A. Mackenzie, Mackenzie, John A.,, John A Mackenzie, Veltman, Douwe M.,, Mackenzie, John A., Veltmahn, Douwe M., Veltman, Douwe M., Steven D. Webb, Robert H. Insall   +26 more
core   +1 more source

ER exit sites mediated by the COPII adaptor sec24D selectively recruit lipid raft-preferring proteins for rapid ER export

Nature Communications
The determinants of sub-cellular trafficking for many membrane proteins are poorly understood. Lipid-driven membrane nanodomains known as lipid rafts have been widely implicated in post-Golgi traffic, but their involvement in protein sorting in the ...
Ivan Castello-Serrano, Shikha Dagar, Rossana Ippolito, Kandice R. Levental, Ilya Levental   +4 more
doaj   +1 more source