Results 81 to 90 of about 446,721 (287)

The exocyst is required for trypanosome invasion and the repair of mechanical plasma membrane wounds [PDF]

, 2015
The process of host cell invasion by Trypanosoma cruzi shares mechanistic elements with plasma membrane injury and repair. Both processes require Ca2+-triggered exocytosis of lysosomes, exocytosis of acid sphingomyelinase and formation of ceramide ...
Corrotte, Matthias, Fernandes, Maria Cecilia, Miguel, Danilo C., Tam, Christina, Andrews, Norma W.   +4 more
core   +1 more source

Membrane repair following filtroporation-induced cell permeabilization

iScience
Mechanical disruption of cell membranes to enable intracellular delivery has been gaining traction as a methodology. Here, we show that a mechanical disruption-based strategy, filtroporation (FP), can be applied to edit the beta globin gene efficiently in human hematopoietic stem and progenitor cells.
Isaura M. Frost, Ruby Sims, Anya Yakimenko, Rachel Ma, Maximilian Floridia, Ruth A. Foley, Emily C. Duggan, Kelly Y. Cai, Kelsey Jorgensen, Emily Skuratovsky, Paul S. Weiss, Steven J. Jonas   +11 more
openaire   +2 more sources

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

Journal of Visualized Experiments, 2014
The ability of injured cells to heal is a fundamental cellular process, but cellular and molecular mechanisms involved in healing injured cells are poorly understood. Here assays are described to monitor the ability and kinetics of healing of cultured cells following localized injury.
Aurelia, Defour, S C, Sreetama, Jyoti K, Jaiswal   +2 more
openaire   +3 more sources

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Nuclear EGFR modulation of DNA repair [PDF]

, 2011
Overexpression of the epidermal growth factor receptor (EGFR) is associated with resistance to chemotherapy and radiotherapy. EGFR involvement, in repair of radiation-induced DNA damage, is mediated by association with the catalytic subunit of DNA ...
Liccardi, G.
core  

Bacterial pore-forming toxin pneumolysin: Cell membrane structure and microvesicle shedding capacity determines differential survival of immune cell types [PDF]

, 2020
Bacterial infectious diseases can lead to death or to serious illnesses. These outcomes are partly the consequence of pore‐forming toxins, which are secreted by the pathogenic bacteria (eg, pneumolysin of Streptococcus pneumoniae).
Babiychuk, Eduard B., Iacovache, Mircea Ioan, Larpin, Yu, Besançon, Hervé, Draeger, Annette, Babiychuk, Victoriia S., Köffel, René, Zuber, Benoît   +7 more
core   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

The effect of modulating membrane lipid composition on the thermal sensitivity of tumour cells in culture [PDF]

, 1989
The plasma membrane, which separates intracellular contents from extra cellular milieu, consists of a lipid bilayer comprising mainly phospholipids and cholesterol together with various functional proteins, which control the interaction of the cell with ...
Kingston, Catharine Alison
core  

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source