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Methylglyoxal and cell viability

International Journal of Biochemistry, 1994
Methylglyoxal by depleting glutathione stores increased Trypan-blue uptake by the cells incubated in glucose, pyruvate and amino acids free medium. Only a transient fall of glutathione concentration without any effect on cell viability was caused by methylglyoxal when the medium was supplemented with above-mentioned compounds.
L, Braun   +4 more
openaire   +2 more sources

Automatic Cell Counter for cell viability estimation [PDF]

open access: possible2014 37th International Convention on Information and Communication Technology, Electronics and Microelectronics (MIPRO), 2014
Despite several methods that exist in different fields of life sciences, certain biotechnological applications still require microscopic analysis of the samples and in many instances, counting of cells. Some of those are drug delivery, transfection or analysis of mechanism fluorescent probes are used to detect cell viability, efficiency of a specific ...
Jasna Lojk   +3 more
openaire   +1 more source

Measurement of Cell Viability

2012
An overview of the methods for assessing cell viability is presented. Different protocols of the most commonly used assays are described in detail so that the readers may be able to determine which assay is suitable for their own projects in plant biotechnology.
Lizbeth A, Castro-Concha   +2 more
openaire   +2 more sources

Bioluminescence assay for cell viability

Biochemistry (Moscow), 2015
Theoretical aspects of the adenosine triphosphate bioluminescence assay based on the use of the firefly luciferin-luciferase system are considered, as well as its application for assessing cell viability in microbiology, sanitation, medicine, and ecology.
G Yu, Lomakina   +2 more
openaire   +2 more sources

Control of T Cell Viability

Annual Review of Immunology, 2004
▪ Abstract  The factors affecting T cell viability vary depending on the type and status of the T cell involved. Naïve T cells die via a Bcl-2/Bim dependent route. Their deaths are prevented in animals by IL-7 and contact with MHC. Activated T cells die in many different ways. Among these is a pathway involving signals that come from outside the T cell
Philippa, Marrack, John, Kappler
openaire   +2 more sources

In vitro chondrogenesis and cell viability

Developmental Biology, 1974
Abstract Anterior somites cultured with (NSA) or without (SA) notochord, and posterior somites cultured with (NSP) or without notochord (SP) were compared with respect to changes in their DNA content, their potential to synthesize the active sulfate principle phosphoadenosine phosphosulfate (PAPS), and their ability to accumulate 35 S-sulfate ...
J S, Gordon, J W, Lash
openaire   +2 more sources

Assessment of Cell Viability

Current Protocols in Cytometry, 2001
AbstractCell viability may be judged by morphological changes or by changes in membrane permeability and/or physiological state inferred from the exclusion of certain dyes of the uptake and retention of others. This unit presents methods based on dye exclusion, esterase activity, and mitochondrial membrane potential, as well as protocols for ...
openaire   +2 more sources

Beauvericin Decreases Cell Viability of Wheat

Chemistry & Biodiversity, 2009
AbstractRecently, beauvericin (BEA) has been recognized as an important toxic compound synthesized by several Fusarium strains, infecting maize, wheat, and rice, worldwide. The effects of BEA on mammalian cells have been studied; however, its effects on the function of host plant cells are largely unknown.
Srobarova A.   +4 more
openaire   +4 more sources

Effects on Cell Viability

2005
Cannabinoids are known to control the cell survival/death decision, leading to different outcomes that depend on the nature of the target cell and its proliferative or differentiation status. Cannabinoids induce growth arrest or apoptosis in a number of transformed cells in culture.
openaire   +2 more sources

Multiplexing Cell Viability Assays

2011
Today, obtaining mechanistic insights into biological, toxicological, and pathological processes is of upmost importance. Researchers aim to obtain as many as possible data from one cell sample to understand the biological processes under study. Multiplexing, which is the ability to gather more than one set of data from the same sample, fulfills ...
Helga H J, Gerets   +2 more
openaire   +2 more sources

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