Results 271 to 280 of about 1,019,407 (314)

Assessment of Beta Cell Viability

Current Protocols in Cytometry, 2011
AbstractThis unit contains detailed protocols for the simultaneous identification of the human pancreatic β cells and determination of their viability by flow cytometry. The enumeration of β cells is based on the ability of the cell‐permeable form of the zinc‐selective dye, FluoZin‐3‐AM, to bind intracellular labile zinc stored at higher levels in ...
openaire   +2 more sources

Photobiomodulation alters the viability of HUVECs cells

Lasers in Medical Science, 2020
The aim of the present study was to investigate the influence of low-level red (660 nm) and infrared (780 nm) laser with four different radiance exposures on human umbilical vein endothelial cells (HUVECs) in vitro. HUVECs (1.5 × 104) were incubated in 96-well culture plates.
Stella Maris Lins Terena, Raquel Agnelli Mesquita-Ferrari, Andreia Martinelli de Siqueira Araújo, Kristianne Porta Santos Fernandes, Maria Helena Fernandes   +4 more
openaire   +2 more sources

Motility, viability, and calcium in the sperm cells

Systems Biology in Reproductive Medicine, 2013
Sperm cells are complicated in vitro models. Their viability is limited, and physiology is complex. The study of their properties is of great application in the animal production as viable and functional gametes are essential. It has been shown that the decrease of sperm cell viability parallels an increase of the reactive oxygen species (ROS ...
openaire   +2 more sources

Maintaining dendritic cell viability in culture

Molecular Immunology, 2015
When mouse dendritic cells (DCs) are isolated from tissues, purified and placed in a nutritive culture they die more rapidly than would be expected from their normal turnover in vivo. This can distort culture assays of DC function. We therefore tested several approaches to prolonging DC survival in culture.
David Vremec, Jacinta Hansen, Andreas Strasser, Hans Acha-Orbea, Yifan Zhan, Meredith O’Keeffe, Ken Shortman   +6 more
openaire   +3 more sources

Cell Viability Assays: Introduction

2011
The measurement of cell viability plays a fundamental role in all forms of cell culture. Sometimes it is the main purpose of the experiment, such as in toxicity assays. Alternatively, cell viability can be used to -correlate cell behaviour to cell number, providing a more accurate picture of, for example, anabolic -activity.
openaire   +2 more sources

Criteria of Viability of Isolated Liver Cells

Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1975
36.4 +/Various cellular parameters were measured with regard to their usefulness as criteria of viability of isolated cells. Stainability by trypan blue and release of lactate dehydrogenase indicate only severe irreversible damage of cells. Neither endogenous respiration nor even the ATP/ADP ratio is a sensitive criterion of viability.
H, Baur, S, Kasperek, E, Pfaff
openaire   +2 more sources

Viability measurements in mammalian cell systems

Analytical Biochemistry, 1989
Abstract The term “cellular viability” and the diverse assays used to determine viability can strongly influence the interpretation of experimental results. With such one-component systems as purified enzymes, an effect usually can be assigned unambiguously to the modification of a specific enzymatic property.
J A, Cook, J B, Mitchell
openaire   +2 more sources

Influence of mechanical stress on cell viability

Biorheology: The Official Journal of the International Society of Biorheology, 2006
The cartilage is a hydrated connective tissue in joints that withstands and distributes mechanical forces. The chondrocytes utilize mechanical signals to regulate their metabolic activity through complex biological and biophysical interactions with the extracellular matrix (ECM).
C, Huselstein, N, de Isla, M N, Kolopp-Sarda, H, Kerdjoudj, S, Muller, J F, Stoltz   +5 more
openaire   +2 more sources

Monitoring Cell Growth, Viability, and Apoptosis

2013
The accurate determination of cell growth and viability is pivotal to monitoring a bioprocess. Direct methods to determine the cell growth and/or viability in a bioprocess include microscopic counting, electronic particle counting, image analysis, in situ biomass monitoring, and dieletrophoretic cytometry.
Michael, Butler, Maureen, Spearman, Katrin, Braasch   +2 more
openaire   +2 more sources

Electrochemical assay of mammalian cell viability

Bioelectrochemistry
We present the first use of amperometric detection to assess the viability of mammalian cells in continuous mode, directly in the cell culture medium. Vero or HeLa cells were injected into electrochemical sensors equipped with a 3-electrode system and containing DCIP 50 µM used as the redox mediator. DCIP was reduced by the viable cells and the reduced
Simon Guette-Marquet, Valentin Saunier, Ludovic Pilloux, Christine Roques, Alain Bergel   +4 more
openaire   +2 more sources