Results 21 to 30 of about 5,277,948 (253)

Viability Assays for Cells in Culture [PDF]

Journal of Visualized Experiments, 2014
Manual cell counts on a microscope are a sensitive means of assessing cellular viability but are time-consuming and therefore expensive. Computerized viability assays are expensive in terms of equipment but can be faster and more objective than manual cell counts. The present report describes the use of three such viability assays.
Jessica M, Posimo, Ajay S, Unnithan, Amanda M, Gleixner, Hailey J, Choi, Yiran, Jiang, Sree H, Pulugulla, Rehana K, Leak   +6 more
openaire   +2 more sources

Recommendations for Validation of Automated Viable Cell Counting Methods (Review)

Разработка и регистрация лекарственных средств, 2023
Introduction. The quality of viable cell-based products (such as biomedical cell products and advanced therapy medicinal products) must be maintained during the full production cycle to ensure their safety and efficacy for patients.
M. A. Vodyakova, N. S. Pokrovsky, E. V. Melnikova, V. A. Merkulov   +3 more
doaj   +1 more source

Application of additive manufacturing in the biomedical field- A review

Annals of 3D Printed Medicine, 2023
Three dimensional (3D) bioprinting is a rapidly developing and emerging field in medicine and engineering. It allows for the production of three-dimensional structures that mimic body tissue function, which can be used in drug delivery and medical ...
A. Harding, A. Pramanik, A.K. Basak, C. Prakash, S. Shankar   +4 more
doaj   +1 more source

Antioxidant-Rich Extracts of Terminalia ferdinandiana Interfere with Estimation of Cell Viability

Antioxidants, 2019
The impact of plant extracts and phytochemicals on in vitro cell viability is usually assessed by employing cell viability assays dependent upon the activity of dehydrogenase enzymes.
Saleha Akter, Rama Addepalli, Michael E. Netzel, Ujang Tinggi, Mary T. Fletcher, Yasmina Sultanbawa, Simone A. Osborne   +6 more
doaj   +1 more source

Development of a flow cytometric method to analyze subpopulations of bacteria in probiotic products and dairy starters [PDF]

, 2002
Flow cytometry (FCM) is a rapid and sensitive technique that can determine cell numbers and measure various physiological characteristics of individual cells by using appropriate fluorescent probes.
Abee, T., Bunthof, C.J.
core   +1 more source

Viability and Adhesion of Periodontal Ligament Fibroblasts on a Hydroxyapatite Scaffold Combined with Collagen, Polylactic Acid–Polyglycolic Acid Copolymer and Platelet-Rich Fibrin: A Preclinical Pilot Study

Dentistry Journal, 2022
Background: Conventional periodontal therapy relies on bone regeneration strategies utilizing scaffolds made of diverse materials, among which collagen, to promote cell adhesion and growth.
Leonor C. Espitia-Quiroz, Andrés L. Fernández-Orjuela, Lina M. Anaya-Sampayo, Adriana P. Acosta-Gómez, Luis Gonzalo Sequeda-Castañeda, Sandra Janeth Gutiérrez-Prieto, Nelly S. Roa-Molina, Dabeiba A. García-Robayo   +7 more
doaj   +1 more source

Integrating Transcriptomics and 3D Spheroid Models Reveals Microenvironment-Dependent Purinergic Modulation in Hepatocellular Carcinoma

Metabolites
Background/Objectives: Dysregulation of purinergic signaling, particularly CD73 overexpression, influences tumor progression, immune evasion, and chemoresistance in hepatocellular carcinoma (HCC). We aimed to characterize the transcriptional landscape of
Arieli Cruz de Sousa, Augusto Ferreira Weber, Vinícius Klain, Juliete Nathali Scholl, Jéssica Marques Obelar Ramos, Natália Baltazar do Nascimento, Maria Luiza Giehl, Renata Kruger Martins, João Vitor Heres, Camila Kehl Dias, Renata Marschner, Fabrício Figueiró, Fátima Costa Rodrigues Guma   +12 more
doaj   +1 more source

Effects of diluents on cell culture viability measured by automated cell counter. [PDF]

PLoS ONE, 2017
Commercially available automated cell counters based on trypan blue dye-exclusion are widely used in industrial cell culture process development and manufacturing to increase throughput and eliminate inherent variability in subjective interpretation ...
Aaron Chen, Matthew Leith, Roger Tu, Gurpreet Tahim, Anish Sudra, Swapnil Bhargava   +5 more
doaj   +1 more source

Determining the Influence of Small Molecules on Hypoxic Prostate Cancer Cell (DU-145) Viability Using Automated Cell Counting and a Cell Harvesting Protocol

Bio-Protocol, 2016
Cell viability assays are an essential aspect of most cancer studies, however they usually require a considerable labor and time input. Here, instead of using the conventional microscopy and hemocytometer cell counting approach, we developed a cell ...
John Phelan, F Jerry Reen, Fergal O’Gara   +2 more
doaj   +1 more source

6,3'-dimethoxy flavonol: Evidence-based insights into anti-proliferative and apoptotic effect on osteosarcoma cells

Trakya University Journal of Natural Sciences
Osteosarcoma, a cancer predominantly affecting children and teenagers, has shown limited improvements in long-term survival rates despite advances in treatment, necessitating new therapeutic approaches.
Elumalai Perumal, Vijaykumar Sayeli, Lokesh Kumar Harikrishnan, Jayavarthini Jayaraman, Srinithi Manikandan, Binoy Cheriyan, Kaniga Pandi   +6 more
doaj   +1 more source