Results 31 to 40 of about 5,277,948 (253)
Stem Cell Imaging: Tools to Improve Cell Delivery and Viability
Stem Cells International, 2016 Stem cell therapy (SCT) has shown very promising preclinical results in a variety of regenerative medicine applications. Nevertheless, the complete utility of this technology remains unrealized. Imaging is a potent tool used in multiple stages of SCT and
Junxin Wang, Jesse V. Jokerst
doaj +1 more source
In Vitro Cytotoxicity of 7,3′,4′-Trihydroxyflavones in Lung Fibroblasts
Medical Sciences Forum, 2022 According to the World Health Organization (WHO), cancer is the second cause of death globally. Conventional cancer therapy includes surgery, chemotherapy, and radiotherapy.
Isabela Santos +4 more
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
A machine vision-based in situ probe for automated on-line measurement of cell density and viability by dark field microscopy, image processing and pattern recognition [PDF]
, 2007 Wei N. A machine vision-based in situ probe for automated on-line measurement of cell density and viability by dark field microscopy, image processing and pattern recognition.
Wei, Ning
core
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Validation of three viable-cell counting methods: Manual, semi-automated, and automated
Biotechnology Reports, 2015 A viable cell count is essential to evaluate the kinetics of cell growth. Since the hemocytometer was first used for counting blood cells, several variants of the methodology have been developed towards reducing the time of analysis and improving ...
Daniela Cadena-Herrera +6 more
doaj +1 more source
Hydrogen generating patch improves skin cell viability, migration activity, and collagen expression
Engineered Regeneration, 2020 Molecular hydrogen recently attracted lots of attention in biomedical community because of its abilities to modulate signal transduction, protein phosphorylation, and gene expression that enable its anti-inflammatory, anti-allergy, and anti-apoptotic ...
Marina Safonov +5 more
doaj +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Cellular & Molecular Biology Letters, 2019 Background In recent years, microRNA-211 (miR211) has been considered as a tumor suppressor in multiple malignancies. However, the function of miR211 in human osteosarcoma has not been explored intensively so far.
Yihua Pei +4 more
doaj +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source