Results 11 to 20 of about 3,387 (178)

Engineering enhanced cellobiohydrolase activity [PDF]

open access: yesNature Communications, 2018
Cellobiohydrolases (CBHs) are critical for natural and industrial biomass degradation but their structure–activity relationships are not fully understood.
Larry E. Taylor   +18 more
doaj   +4 more sources

High level secretion of cellobiohydrolases by Saccharomyces cerevisiae [PDF]

open access: yesBiotechnology for Biofuels, 2011
Background The main technological impediment to widespread utilization of lignocellulose for the production of fuels and chemicals is the lack of low-cost technologies to overcome its recalcitrance.
Ahlgren Simon   +16 more
doaj   +5 more sources

Catalytic mechanism of cellulose degradation by a cellobiohydrolase, CelS.

open access: yesPLoS ONE, 2010
The hydrolysis of cellulose is the bottleneck in cellulosic ethanol production. The cellobiohydrolase CelS from Clostridium thermocellum catalyzes the hydrolysis of cello-oligosaccharides via inversion of the anomeric carbon.
Moumita Saharay   +2 more
doaj   +4 more sources

Processivity of Cellobiohydrolases Is Limited by the Substrate [PDF]

open access: yesJournal of Biological Chemistry, 2011
Processive cellobiohydrolases (CBHs) are the key components of fungal cellulase systems. Despite the wealth of structural data confirming the processive mode of action, little quantitative information on the processivity of CBHs is available. Here, we developed a method for measuring cellulase processivity.
Mihhail, Kurasin, Priit, Väljamäe
openaire   +2 more sources

Localization of C Cycle Enzymes in Arable and Forest Phaeozems within Levels of Soil Microstructure

open access: yesMicroorganisms, 2023
Soil microbial and enzyme activities are closely related to the spatial variability of soil environmental conditions at the microscale (μm-mm). The origin and localization of the enzymes are somewhat neglected when the measured activity is used to ...
Anna Yudina   +3 more
doaj   +1 more source

Effect of Pretreatments on the Production of Biogas from Castor Waste by Anaerobic Digestion

open access: yesFermentation, 2023
Lignocellulosic biomass is a source of carbohydrates that can be used in the production of biogas. The aim of this study was to obtain biogas from biomass waste (leaves, stems and seed bagasse) of Ricinus communis, applying pretreatments such as ...
Diana Laura Quezada-Morales   +10 more
doaj   +1 more source

Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion

open access: yesArab Journal of Basic and Applied Sciences, 2019
The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose ...
Romina O. Coniglio   +6 more
doaj   +1 more source

Combinatorial Screening for Transgenic Yeasts with High Cellulase Activities in Combination with a Tunable Expression System. [PDF]

open access: yesPLoS ONE, 2015
Combinatorial screening used together with a broad library of gene expression cassettes is expected to produce a powerful tool for the optimization of the simultaneous expression of multiple enzymes.
Yoichiro Ito   +4 more
doaj   +1 more source

Chimeric cellobiohydrolase I expression, activity, and biochemical properties in three oleaginous yeast

open access: yesBiotechnology for Biofuels, 2021
Consolidated bioprocessing using oleaginous yeast is a promising modality for the economic conversion of plant biomass to fuels and chemicals. However, yeast are not known to produce effective biomass degrading enzymes naturally and this trait is ...
Markus Alahuhta   +9 more
doaj   +1 more source

Recombinant Family 1 Carbohydrate-Binding Modules Derived From Fungal Cellulase Enhance Enzymatic Degradation of Lignocellulose as Novel Effective Accessory Protein

open access: yesFrontiers in Microbiology, 2022
Fungal cellulases usually contain a family 1 carbohydrate-binding module (CBM1), and its role was considered to recognize the substrate specifically. This study testified that the CBM1s derived from cellobiohydrolase I of Trichoderma reesei, Penicillium ...
Hexue Jia   +6 more
doaj   +1 more source

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