Results 21 to 30 of about 3,387 (178)

Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi [PDF]

Biochemical Journal, 1995
The gene cbhB from the cellulolytic bacterium Cellulomonas fimi encodes a polypeptide of 1090 amino acids. Cellobiohydrolase B (CbhB) is 1037 amino acids long, with a calculated molecular mass of 109765 Da. The enzyme comprises five domains: an N-terminal catalytic domain of 643 amino acids, three fibronectin type III repeats of 97 amino acids each ...
H, Shen, N R, Gilkes, D G, Kilburn, R C, Miller, R A, Warren   +4 more
openaire   +2 more sources

Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum

Frontiers in Energy Research, 2018
To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O ...
Lan Yao, Lan Yao, Lan Yao, Haitao Yang, Haitao Yang, Chang Geun Yoo, Xianzhi Meng, Yunqiao Pu, Naijia Hao, Arthur J. Ragauskas, Arthur J. Ragauskas, Arthur J. Ragauskas   +11 more
doaj   +1 more source

Cellobiohydrolase from Trichoderma reesei [PDF]

Biochemical Journal, 1983
A 1,4-beta-D-glucan cellobiohydrolase (EC 3.2.1.91) was purified from the culture liquid of Trichoderma reesei by using biospecific sorption on amorphous cellulose and immunoaffinity chromatography. A single protein band in polyacrylamide-gel electrophoresis and one arc in immunoelectrophoresis corresponded to the enzyme activity.
M, Nummi, M L, Niku-Paavola, A, Lappalainen, T M, Enari, V, Raunio   +4 more
openaire   +2 more sources

Cloning and expression analyses of a cellobiohydrolase gene from Auricularia heimuer

Biotechnology & Biotechnological Equipment, 2019
A cellobiohydrolase gene was cloned and analyzed from Auricularia heimuer, and named A-cbh. The cDNA sequence was submitted to GenBank under the accession number MK620906.
Jian Sun, Shixin Wang, Xutong Wang, Tingting Sun, Li Zou   +4 more
doaj   +1 more source

Screening of new secretory cellulases from different supernatants of white rot fungi from Misiones, Argentina

Mycology, 2017
Cellulases hydrolyse the cellulose chain into single sugars efficiently. These sugars can be fermented in the bioethanol process, a source of renewable energy. Misiones rainforest is one of the most biodiverse systems on the planet subtropical ecoregions,
Romina Olga Coniglio, María Isabel Fonseca, Laura Lidia Villalba, Pedro Darío Zapata   +3 more
doaj   +1 more source

Use of nanostructure initiator mass spectrometry (NIMS) to deduce selectivity of reaction in glycoside hydrolases

Frontiers in Bioengineering and Biotechnology, 2015
Chemically synthesized nanostructure-initiator mass spectrometry (NIMS) probes derivatized with tetrasaccharides were used to study the reactivity of representative Clostridium thermocellum β-glucosidase, endoglucanases and cellobiohydrolase.
Kai eDeng, Kai eDeng, Taichi E. Takasuka, Taichi E. Takasuka, Christopher M. Bianchetti, Christopher M. Bianchetti, Lai F. Bergeman, Paul D. Adams, Paul D. Adams, Paul D. Adams, Trent Russell Northen, Trent Russell Northen, Brian Grant Fox, Brian Grant Fox   +13 more
doaj   +1 more source

Enzyme-Mediated Lignocellulose Liquefaction Is Highly Substrate-Specific and Influenced by the Substrate Concentration or Rheological Regime

Frontiers in Bioengineering and Biotechnology, 2020
The high viscosities/yield stresses of lignocellulose slurries makes their industrial processing a significant challenge. However, little is known regarding the degree to which liquefaction and its enzymatic requirements are specific to a substrate’s ...
Timo van der Zwan, Alexander Sigg, Alexander Sigg, Jinguang Hu, Richard P. Chandra, Jack N. Saddler   +5 more
doaj   +1 more source

Simultaneous enhancement of the beta–exo synergism and exo–exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability

Microbial Cell Factories, 2019
Background Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries. As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and ...
Hao Fang, Runze Zhao, Chaofeng Li, Chen Zhao   +3 more
doaj   +1 more source

Modeling the minimum enzymatic requirements for optimal cellulose conversion

Environmental Research Letters, 2013
Hydrolysis of cellulose is achieved by the synergistic action of endoglucanases, exoglucanases and β-glucosidases. Most cellulolytic microorganisms produce a varied array of these enzymes and the relative roles of the components are not easily defined or
R den Haan, J M van Zyl, T M Harms, W H van Zyl   +3 more
doaj   +1 more source

Trade-Offs Between Growth Rate and Other Fungal Traits

Frontiers in Forests and Global Change, 2021
If we better understand how fungal responses to global change are governed by their traits, we can improve predictions of fungal community composition and ecosystem function.
Karissa G. Lovero, Kathleen K. Treseder
doaj   +1 more source