Results 111 to 120 of about 9,990 (205)

Is HPC for you? A collective perspective on adopting high‐performance computing for bioimage analysis in imaging facilities

Journal of Microscopy, EarlyView.
Abstract Biomedical sciences use various imaging technologies and bioimage analysis has become data‐intensive, often requiring resources that exceed those available on personal laptop computers or workstations. High‐performance computing (HPC) can solve most of the problems connected to the data burden of modern bioimaging, offering scalable and ...
Rocco D'Antuono, Tatiana Woller, Benjamin Pavie, Andrew Collins, Paul A. Wilkinson, Marie Held, Konstantin B. Borisenko, Chiara Rossi, Sebastian Munck, Marco Marcello   +9 more
wiley   +1 more source

A Deep Learning Pipeline for Cell Segmentation and Viability Quantification in 3D Constructs From Fluorescence Microscopy Images

Microscopy Research and Technique, Volume 89, Issue 11, Page 1327-1338, November 2026.
3D bioprinting requires advanced methods for cell localization and viability assessment. We developed a hybrid and automated pipeline based on the U 2‐Net architecture for the analysis of fluorescence microscopy images enabling cell segmentation and viability assessment. The model was used to track cell viability in 3D constructs demonstrating improved
Federica Valtellina, Francesco Iannacci, Bianca Maria Colosimo, Mattia Sponchioni   +3 more
wiley   +1 more source

Depot‐Specific Insulin Receptor Isoform Dynamics During Human Adipose‐Derived Stem Cell Adipogenesis

Obesity, Volume 34, Issue 11, Page 2124-2134, November 2026.
Absolute quantification reveals depot‐specific insulin receptor (IR) isoform signatures in human adipose stem cells, with subcutaneous cells enriched in IRa and exhibiting greater adipogenic capacity and insulin responsiveness. The emergence of IR‐negative adipocytes highlights partially receptor‐independent pathways regulating human adipogenesis ...
Valentina Annamaria Genchi, Celeste Lauriola, Cristina Caccioppoli, Rossella D'Oria, Nicola Marrano, Giuseppina Biondi, Sebastio Perrini, Annalisa Natalicchio, Luigi Laviola, Francesco Giorgino, Angelo Cignarelli   +10 more
wiley   +1 more source

Programmable Functional Silicification of DNA Origami Nanostructures

Advanced Materials, Volume 38, Issue 56, 8 October 2026.
This work introduces additional functionality into silica‐coated DNA origami nanostructures using non‐standard silica precursors. A fluorescent precursor enables enhanced intracellular tracking, while a disulfide‐containing reagent yields redox‐responsive, degradable silica coatings.
Anna V. Baptist, Lasse Guericke, Philipp Mauker, Oliver Thorn‐Seshold, Amelie Heuer‐Jungemann   +4 more
wiley   +1 more source

Spatial Control of Light‐Responsive Proteins and Optogenetics Within Hydrogels via Volumetric Bioprinting

Advanced Materials, Volume 38, Issue 60, 27 October 2026.
Spatiotemporal control over cell behavior within bioprinted constructs remains a key challenge in biofabrication. Volumetric bioprinting using multi‐wavelength light sources permits to activate light‐sensitive intracellular proteins as well as optogenetic gene and protein expression circuits in bioprinted cells, in addition to enabling fast printing of
Davide Ribezzi, Pere Català, Gabriel Größbacher, Paulina Nuñez Bernal, Olaf Nijssen, Sammy Florczak, Wilco Nijenhuis, Nuria Crusellas‐Villorbina, Bram Nijhoff, Paul Delrot, Jos Malda, Andreas Hierholzer, Martin Fussenegger, Lukas C. Kapitein, Riccardo Levato   +14 more
wiley   +1 more source

Label-free droplet image analysis with Cellprofiler

Abstract Droplet microfluidic methods used for microbiological experiments are fast, cost-effective, and provide high-throughput data. However, analysis of such image data can be difficult, and detection of molecular labels is limited by microscope parameters.
Dániel Kácsor, Merili Saar-Abroi, Triini Olman, Simona Bartkova, Ott Scheler   +4 more
openaire   +1 more source

CellProfiler pipeline for autophagosome counting and cell analysis

A CellProfiler pipeline to count autophagosomes and quantify other cell features.
Collins, Adam
core   +1 more source

S‐Nitrosothiols as Thiol‐Protecting Groups for Controlled Thiol‐Maleimide Crosslinking of Homogeneous Soft Hydrogels

Advanced Materials, Volume 38, Issue 57, 13 October 2026.
Rapid thiol‐maleimide addition frequently outpaces mixing, resulting in heterogeneous hydrogels. S‐nitrosothiols act as thiol‐protecting groups, allowing uniform mixing with maleimide‐functionalized polymers before gelation. Sodium thiosulfate or sodium ascorbate then regenerates thiols on demand, triggering controlled thiol‐maleimide crosslinking ...
Julian A. Serna, Michelle J. Iwohn, Maximilian Seifermann, Stefan Heißler, Maike Schliephake, Franziska Dopp, Claudia Muhle‐Goll, Pavel A. Levkin   +7 more
wiley   +1 more source

Multispectral Analysis Graphical User Interface for Ratiometric Analysis and Background Correction

Chemistry–Methods, Volume 6, Issue 10, October 2026.
This article describes multispectral analysis graphical user interface, an open‐source, cross‐platform graphical application for highly modular batch image analysis and visualization that requires no coding experience. With common image operations as a palette, users can define their own analysis pipeline (e.g., background subtraction into ...
Grayson R. Hoy, Caitlin M. Davis
wiley   +1 more source

Live‐cell FRET imaging‐based efficacy prediction of EGFR‐TKIs in EGFR L858R‐mutant NSCLC

Clinical and Translational Medicine, Volume 16, Issue 10, October 2026.
At the single‐cell level, live‐cell FRET microscopy was used to integrate the EGFRL858R‐GRB2 interaction with cellular phenotypic features to predict the efficacy of EGFR‐TKIs. The EGFRL858R‐GRB2 interaction‐based T‐score can evaluate the on‐target activity of EGFR‐TKIs against EGFRL858R.
Xin Li, Lingao Dai, Pingshan Yang, Shuanghe Li, Chuan Peng, Shiyao Li, Beini Sun, Songwang Cai, Xiaoping Wang, Tongsheng Chen   +9 more
wiley   +1 more source