Results 51 to 60 of about 9,990 (205)

Additional file 1: of Automated muscle histopathology analysis using CellProfiler

, 2018
MuscleAnalyzer pipeline (ND2) for CellProfiler version 2.2.0.
Renzhi Han (276195), Yeh Siang Lau (841820), Yandi Gao (3500792), Li Xu (108702)   +3 more
core   +1 more source

A High-Content Larval Zebrafish Brain Imaging Method for Small Molecule Drug Discovery. [PDF]

PLoS ONE, 2016
Drug discovery in whole-organisms such as zebrafish is a promising approach for identifying biologically-relevant lead compounds. However, high content imaging of zebrafish at cellular resolution is challenging due to the difficulty in orienting larvae ...
Harrison Liu, Steven Chen, Kevin Huang, Jeffrey Kim, Han Mo, Raffael Iovine, Julie Gendre, Pauline Pascal, Qiang Li, Yaping Sun, Zhiqiang Dong, Michelle Arkin, Su Guo, Bo Huang   +13 more
doaj   +1 more source

SPHINX31 acts as a SRPK1 inhibitor targeting the ATR/DNA‐PKcs/CHK1 replicative checkpoint to inhibit cell growth in non‐small cell lung cancer

Molecular Oncology, EarlyView.
The kinase SRPK1 directly interacts with the protein TOPBP1 and regulates the pre‐mRNA splicing of WIZ thereby contributing to the activation of the ATR/CHK1 replicative checkpoint in response to replicative stress. This allows cancer cells' genomic stability and survival.
Amani Shreim, Aurelie Genoux, Nadiia Zubchuk, Haoyang Zheng, Perla Salameh, Theo Ziegelmeyer, Fabien Dalonneau, Margot Rouchette, Christiane Oddou, Simona Miron, Helene Polveche, Adiilah Mamode Cassim, Carmen Garrido, Eleni Nikolakaki, Didier Auboeuf, Tao Jia, Sophie Zinn‐Justin, Beatrice Eymin   +17 more
wiley   +1 more source

CellProfiler iPS colony counting pipeline

, 2019
With this script, iPS or other pluripotent colonies stained for up to two markers can be counted automatically with CellProfiler (v.
PR, Georgina, Peñalosa Ruiz, G (via Mendeley Data)   +1 more
core   +2 more sources

Additional file 3: of Automated muscle histopathology analysis using CellProfiler

, 2018
MuscleAnalyzer pipeline (TIFF) for CellProfiler version 3.0.0.
Renzhi Han (276195), Yeh Siang Lau (841820), Yandi Gao (3500792), Li Xu (108702)   +3 more
core   +1 more source

CellProfiler pipeline for nucleolar 5-EU signal analysis from A high-throughput assay for directly monitoring nucleolar rRNA biogenesis

, 2021
Supplementary File 1. Code for 5-EU image analysis (CellProfiler 3.1.9)
Carson J. Bryant (11889839), Mason A. McCool (11889842), Yulia V. Surovtseva (5992673), Susan J. Baserga (11889845), Laura Abriola (1323723)   +4 more
core   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

FindFoci outperforms CellProfiler^a.

, 2014
aCellProfiler was manually trained by adjusting the parameters of the EnhanceOrSuppressFeatures and IdentifyPrimaryObjects modules until the best overall F1-score was achieved (see Methods for parameter ranges).
Alex D. Herbert (502074), Antony M. Carr (155119), Eva Hoffmann (159398)   +2 more
core   +1 more source

Validation of a Cellular Imaging‐Based Method as a Potential Biomarker for SPG4 Hereditary Spastic Paraplegia

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Hereditary Spastic Paraplegia (HSP) comprises a group of rare genetic diseases characterized by length‐dependent axonal degeneration of the corticospinal tracts and dorsal columns, whose main clinical feature is spastic gait. Pathogenic variants in the SPG4 gene cause Spastic Paraplegia Type 4 (SPG4‐HSP), the most common form of HSP.
Gaia Fattorini, Valerio Licursi, Gianmarco Dalla Zanna, Flavio Dal Canto, Melissa Barghigiani, Nunzio Setola, Salvatore Rossi, Antonio Funcis, Filippo M. Santorelli, Gabriella Silvestri, Carlo Casali, Francesca Sardina, Cinzia Rinaldo   +12 more
wiley   +1 more source

Dynamically Actuated Reconfigurable Topographical Surface Enables Active Control of Implant‐Associated Infections

Advanced Functional Materials, EarlyView.
A dynamically actuated reconfigurable topographical surface (DARTS) integrates contact‐mediated bactericidal nanotopography with programmable mechanical actuation to actively disrupt bacterial biofilms. Dynamic surface reconfiguration enhances bacterial killing and suppresses implant‐associated infections in vivo, providing a new strategy for active ...
Mohammad Asadi Tokmedash, Jihun Lee, J. Scott VanEpps, Sungmin Nam, Jouha Min   +4 more
wiley   +1 more source