Results 41 to 50 of about 9,990 (205)

Automated Estimation Parasitemia of Plasmodium berghei Infected Mice using CellProfiler Software [PDF]

, 2014
In this paper, we propose a technique for automatically recording parasitemia of mice infected with Plasmodium berghei by using Cellprofiler. Our purpose is to identify the difference number of parasitemia obtained by CellProfiler and manual assessment ...
Ramadhani, Dwi, Nurhayati, Siti
core   +5 more sources

Traxtile: Interactive editing of cell tracks in time-lapse images

BioTechniques, 2015
Time-lapse imaging can be used to quantify how cells move, divide, and die over time and under defined culture conditions. Open source software packages such as CellProfiler, Icy, and Fiji provide robust and convenient interfaces for performing such ...
Benjamin S. Braun
doaj   +1 more source

Detecting RNA-protein proximity at DNA double-strand breaks using combined fluorescence in situ hybridization with proximity ligation assay

STAR Protocols, 2023
Summary: RNA transcribed at DNA double-strand breaks (DSBs) contributes to accurate DNA repair. Here, using the repair factors 53BP1 and TIRR as examples, we combine the fluorescence in situ hybridization (FISH) and proximity ligation assay (PLA ...
Adele Alagia, Ruth F. Ketley, Monika Gullerova   +2 more
doaj   +1 more source

Machine Learning based histology phenotyping to investigate the epidemiologic and genetic basis of adipocyte morphology and cardiometabolic traits.

PLoS Computational Biology, 2020
Genetic studies have recently highlighted the importance of fat distribution, as well as overall adiposity, in the pathogenesis of obesity-associated diseases.
Craig A Glastonbury, Sara L Pulit, Julius Honecker, Jenny C Censin, Samantha Laber, Hanieh Yaghootkar, Nilufer Rahmioglu, Emilie Pastel, Katerina Kos, Andrew Pitt, Michelle Hudson, Christoffer Nellåker, Nicola L Beer, Hans Hauner, Christian M Becker, Krina T Zondervan, Timothy M Frayling, Melina Claussnitzer, Cecilia M Lindgren   +18 more
doaj   +1 more source

High-content screening of mitochondrial polarization in neural cells derived from human pluripotent stem cells

STAR Protocols, 2022
Summary: We present a high-content screening (HCS) protocol for quantifying mitochondrial activity in live neural cells from human induced pluripotent stem cells (iPSCs). The assessment is based on mitochondrial membrane potential, which is influenced by
Annika Zink, Undine Haferkamp, Annika Wittich, Mathias Beller, Ole Pless, Alessandro Prigione   +5 more
doaj   +1 more source

Autophagy profiling in single cells with open source CellProfiler-based image analysis

Autophagy, 2022
Single cell-based analysis of macroautophagy/autophagy is largely achieved through the use of fluorescence microscopy to detect autophagy-related proteins that associate with autophagic membranes and therefore can be quantified as fluorescent puncta. In this context, an automated analysis of the number and size of recognized puncta is preferable to a ...
David S. Schüssele, Patricia K. Haller, Maximilian L. Haas, Catherine Hunter, Katharina Sporbeck, Tassula Proikas-Cezanne   +5 more
openaire   +3 more sources

sa2c/CellProfiler-RunOnCluster: First release of the CellProfiler RunOnCluster plugin

, 2019
A CellProfiler plugin for running on an HPC ...
Jarno Rantaharju
core   +1 more source

CellProfiler pipeline

, 2019
CellProfiler pipeline for counting the number of nuclei in Hoechst stained image (4x image)
Kitami, Toshimori, Toshimori Kitami (174722), Uyen Thi Tran (8039807), Tran, Uyen Thi   +3 more
core   +1 more source

Automated image analysis of stained cytospins to quantify Schwann cell purity and proliferation.

PLoS ONE, 2020
In response to injury, adult Schwann cells (SCs) re-enter the cell cycle, change their expression profile, and exert repair functions important for wound healing and the re-growth of axons.
Tamara Weiss, Lorenz Semmler, Flavia Millesi, Anda Mann, Maximilian Haertinger, Manuel Salzmann, Christine Radtke   +6 more
doaj   +1 more source

Pharmacological chromatin remodeling enhances response to estrogen therapy in ER+ breast cancer

Molecular Oncology, EarlyView.
Estrogen therapy elicits clinical benefit in ~ 30% of patients with endocrine‐resistant estrogen receptor (ER)‐positive breast cancer. Based on findings that ER transcriptional activation underlies response to estrogen therapy, we tested the effects of epigenetic dysregulation via pharmacological inhibition of histone deacetylases (HDACi).
Anneka L. Johnson Thomas, Barbara Karakyriakou, Patricia C. Muskus, Alyssa M. Roberts, Justin Rendleman, Alexa Warren, Hanxu Lu, Kimberly A. Birmingham, Malone K. Friedman, Abigail E. Goen, Huijuan Yang, Owen M. Wilkins, Fred W. Kolling, Eugene Demidenko, Julie M. Jorns, Brock C. Christensen, Todd W. Miller   +16 more
wiley   +1 more source