ImmunoFISH for Adherent Cultured Mammalian Cells
Bio-Protocol, 2013 This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.
Francesca Rossiello +2 more
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Three dimensional neuronal cell cultures more accurately model voltage gated calcium channel functionality in freshly dissected nerve tissue. [PDF]
PLoS ONE, 2012 It has been demonstrated that neuronal cells cultured on traditional flat surfaces may exhibit exaggerated voltage gated calcium channel (VGCC) functionality.
Yinzhi Lai, Ke Cheng, William Kisaalita
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Homogenization of Mammalian Cultured Cells
The Scientific World Journal, 2002 Satisfactory homogenization of cultured cells is a necessary prerequisite to any fractionation schedule. Protocols are given for homogenization in iso-osmotic (A) and hypo-osmotic (B) media that should be broadly applicable to any cell type and to any ...
John Graham
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Microgravity-induced apoptosis in cultured glial cells
European Journal of Histochemistry, 2010 Apoptosis is a form of naturally occurring cell death that plays fundamental roles during embryonic developement. In adults, it neatly disposes of cells damaged by injuries provoked by external causes such as UV radiation, ionisation and heat shock ...
BM Uva +6 more
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In Vitro Implantation Model Using Human Endometrial SUSD2+ Mesenchymal Stem Cells and Myometrial Smooth Muscle Cells [PDF]
Cell Journal, 2021 Objective: This study evaluated a novel in vitro implantation model using human endometrial mesenchymal stem cells (EMSCs), SUSD2+, and myometrial smooth muscle cells (SMCs) that were co-cultured with mouse blastocysts as the surrogate embryo ...
Marzieh Rahimipour +2 more
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Lethal impacts of cigarette smoke in cultured tobacco cells
Tobacco Induced Diseases, 2011 Background In order to understand and generalize the toxic mechanism of cigarette smoke in living cells, comparison of the data between animal systems and other biological system such as microbial and plant systems is highly beneficial.
Masaru Yukihiro +2 more
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Distinct cell stress responses induced by ATP restriction in quiescent human fibroblasts
Frontiers in Genetics, 2016 Quiescence is the prevailing state of many cell types under homeostatic conditions. Yet, surprisingly little is known about how quiescent cells respond to energetic and metabolic challenges.
Nirupama Yalamanchili +5 more
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Increased hepatic VLDL secretion, lipogenesis, and SREBP-1 expression in the corpulent JCR:LA-cp rat
Journal of Lipid Research, 2001 The corpulent JCR:LA-cp rat (cp/cp) is a useful model for study of the metabolic consequences of obesity and hyperinsulinemia. To assess the effect of hyperinsulinemia on VLDL secretion in this model, we measured rates of secretion of VLDL in perfused ...
Marshall B. Elam +7 more
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FASEB BioAdvances, 2023 Understanding the expected efficacy and safety of a new regenerative therapy requires analysis of the fate of the transplanted cell graft. We have shown that transplantation of autologous cultured nasal epithelial cell sheets onto the middle ear mucosa ...
Yoshiyuki Kasai +4 more
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The Effect of Hypoxic and Normoxic Culturing Conditions in Different Breast Cancer 3D Model Systems
Frontiers in Bioengineering and Biotechnology, 2021 The field of 3D cell cultures is currently emerging, and material development is essential in striving toward mimicking the microenvironment of a native tissue.
Andreas Svanström +11 more
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