Results 91 to 100 of about 365,586 (305)

Getting to the root of plant biology: impact of the Arabidopsis genome sequence on root research [PDF]

, 2010
Prior to the availability of the genome sequence, the root of Arabidopsis had attracted a small but ardent group of researchers drawn to its accessibility and developmental simplicity.
Schiefelbein, John W., Bennett, Malcolm, Benfey, Philip N.   +2 more
core   +1 more source

Gene and library synthesis without amplification: polymerase step reaction (PSR)

BioTechniques, 2015
Current gene synthesis methods often incorporate a PCR amplification step in order to yield final material sufficient for resolution from multiple off-products. These amplification steps can cause stochastic sampling effects that propagate errors in gene
Zhuo-Bin Lee, Christopher Firnhaber, Jesse Clarke, Brian S. DeDecker   +3 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Cryosectioning and immunofluorescence of C. elegans reveals endogenous polyphosphate in intestinal endo-lysosomal organelles

Cell Reports: Methods
Summary: Polyphosphate (polyP) is a ubiquitous polyanion present throughout the tree of life. While polyP’s widely varied functions have been interrogated in single-celled organisms, little is known about the cellular distribution and function of polyP ...
Ellen Quarles, Lauren Petreanu, Anjali Narain, Aanchal Jain, Akash Rai, Joyful Wang, Bryndon Oleson, Ursula Jakob   +7 more
doaj   +1 more source

miR-1/206 downregulates splicing factor Srsf9 to promote C2C12 differentiation

Skeletal Muscle, 2019
Background Myogenesis is driven by specific changes in the transcriptome that occur during the different stages of muscle differentiation. In addition to controlled transcriptional transitions, several other post-transcriptional mechanisms direct muscle ...
Kristen K. Bjorkman, Massimo Buvoli, Emily K. Pugach, Michael M. Polmear, Leslie A. Leinwand   +4 more
doaj   +1 more source

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

Three serendipitous pathways in E. coli can bypass a block in pyridoxal‐5′‐phosphate synthesis

Molecular Systems Biology, 2010
Bacterial genomes encode hundreds to thousands of enzymes, most of which are specialized for particular functions. However, most enzymes have inefficient promiscuous activities, as well, that generally serve no purpose.
Juhan Kim, Jamie P Kershner, Yehor Novikov, Richard K Shoemaker, Shelley D Copley   +4 more
doaj   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems

Nature Communications
Understanding metabolic heterogeneity is the key to uncovering the underlying mechanisms of metabolic-related diseases. Current metabolic imaging studies suffer from limitations including low resolution and specificity, and the model systems utilized ...
Yeran Bai, Carolina M. Camargo, Stella M. K. Glasauer, Raymond Gifford, Xinran Tian, Andrew P. Longhini, Kenneth S. Kosik   +6 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source