Results 271 to 280 of about 2,335,939 (309)
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Cellular uptake and trafficking of antisense oligonucleotides
Nature Biotechnology, 2017Antisense oligonucleotides (ASOs) modified with phosphorothioate (PS) linkages and different 2' modifications can be used either as drugs (e.g., to treat homozygous familial hypercholesterolemia and spinal muscular atrophy) or as research tools to alter gene expression.
Stanley T Crooke +4 more
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Cellular Uptake of Oligodeoxynucleotide Phosphorothioates and Their Analogs
Antisense Research and Development, 1994The uptake and intracellular distribution of oligonucleotide phosphorothioates (S-oligonucleotides) and their analogs by various cells lines were studied using internally 35S-labeled oligonucleotides. Intracellular accumulation starts in the first 2 hr and reaches a maximum at about 16 hr.
J, Temsamani +4 more
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Studies on the cellular uptake of ribonuclease
Journal of the Royal Microscopical Society, 1965SYNOPSISThe uptake of RNase by two types of amoeba and a selection of vertebrate cells has been investigated using the fluorescein‐labelled enzyme. This method is considered satisfactory for the study since the fluorescein label migrated together with the enzyme during electrophoresis, and the labelled RNase retained one half of its enzymatic activity.
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Stable Cellulose Nanospheres for Cellular Uptake
Macromolecular Bioscience, 2011AbstractPure, perfectly spherical cellulose nanoparticles with sizes of ≈80–260 nm can be prepared by dialysis starting from trimethylsilylcellulose (TMSC). The aqueous suspensions obtained are storable for several months. Subsequent covalent labeling of the cellulose nanoparticles with FITC has no influence on particle size, shape, and stability.
Tim, Liebert +3 more
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Cellular Uptake Mechanism of Molecular Umbrella
Bioconjugate Chemistry, 2009Molecular umbrella provided a promising avenue for the design of the intracellular delivery of hydrophilic therapeutic agents. However, the limited understanding of its cellular uptake would be a roadblock to its effective application. Herein, we investigate the ability and mechanism of cellular entry of a fluorescently labeled diwalled molecular ...
Ge, Dongtao +9 more
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Cellular Uptake and Cytotoxicity of Silica Nanotubes
Nano Letters, 2008"Template synthesized" silica nanotubes (SNTs) provide unique features such as end functionalization to control drug release, inner voids for loading biomolecules, and distinctive inner and outer surfaces that can be differentially functionalized for targeting and biocompatibility.
Anjan, Nan +4 more
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Osmotic Regulation of Cellular Glucose Uptake
2007This chapter describes various approaches allowing the study of hyperosmolarity in the functions of 3T3-L1 adipocytes. Hyperosmolarity mimics insulin responses, such as glucose uptake and membrane ruffling, but also antagonizes these insulin effects, which can be evaluated in 3T3-L1 adipocytes.
Gual, Philippe +6 more
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Calcium-dependent oligonucleotide cellular uptake
Biochimie, 1996Oligodeoxynucleotides (ONs) are currently being tested in clinical trials as anti-viral and thrombolytic agents. Although ONs are biologically active in almost every cell line examined, these molecules are inefficiently internalized. In addition, the mechanism of ON transport to the active site in either the cytoplasm or nucleus remains ill-defined ...
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