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The Clostridium cellulovorans Cellulosome
Critical Reviews in Microbiology, 1994The Clostridium cellulovorans cellulosome is comprised of a large, nonenzymatic scaffolding protein called the cellulose binding protein A (CbpA) and a number of endoglucanases/xylanases. The CbpA contains several functional domains, including a signal peptide, a cellulose binding domain (CBD), a hydrophilic domain (HLD) present four times, and a ...
R H, Doi +4 more
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Cellulose, cellulases and cellulosomes
Current Opinion in Structural Biology, 1998The structural complexity and rigidity of cellulosic substrates have given rise to a phenomenal diversity of degradative enzymes--the cellulases. Cellulolytic microorganisms produce a wide variety of different catalytic and noncatalytic enzyme modules, which form the cellulases and act synergistically on their substrate. In some microbes, several types
Bayer, E.A. +3 more
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The rosettazyme: A synthetic cellulosome
Journal of Biotechnology, 2009Cellulose is an attractive feedstock for biofuel production because of its abundance, but the cellulose polymer is extremely stable and its constituent sugars are difficult to access. In nature, extracellular multi-enzyme complexes known as cellulosomes are among the most effective ways to transform cellulose to useable sugars.
Shigenobu, Mitsuzawa +5 more
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Carbohydrate Depolymerization by Intricate Cellulosomal Systems
2023Cellulosomes are multi-enzymatic nanomachines that have been fine-tuned through evolution to efficiently deconstruct plant biomass. Integration of cellulosomal components occurs via highly ordered protein-protein interactions between the various enzyme-borne dockerin modules and the multiple copies of the cohesin modules located on the scaffoldin ...
Sarah Moraïs +5 more
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Cellulosomes: Highly Efficient Cellulolytic Complexes
2020Cellulosomes are elaborate multienzyme complexes capable of efficiently deconstructing lignocellulosic substrates, produced by cellulolytic anaerobic microorganisms, colonizing a large variety of ecological niches. These macromolecular structures have a modular architecture and are composed of two main elements: the cohesin-bearing scaffoldins, which ...
Victor D, Alves +2 more
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Advances in consolidated bioprocessing using synthetic cellulosomes
Current Opinion in Biotechnology, 2022The primary obstacle impeding the more widespread use of biomass for energy and chemical production is the absence of a low-cost technology for overcoming their recalcitrant nature. It has been shown that the overall cost can be reduced by using a 'consolidated' bioprocessing (CBP) approach, in which enzyme production, biomass hydrolysis, and sugar ...
Shen-Long, Tsai +2 more
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International Journal of Biological Macromolecules, 2022
Polysaccharides derived from lignocellulose are promising sustainable carbon sources. Cellulosome is a supramolecular machine integrating multi-function enzymes for effective lignocellulose bio-saccharification. However, how various non-cellulose components of lignocellulose affect the cellulosomal saccharification is hitherto unclear. This study first
Chao, Chen +6 more
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Polysaccharides derived from lignocellulose are promising sustainable carbon sources. Cellulosome is a supramolecular machine integrating multi-function enzymes for effective lignocellulose bio-saccharification. However, how various non-cellulose components of lignocellulose affect the cellulosomal saccharification is hitherto unclear. This study first
Chao, Chen +6 more
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Carbohydrate Depolymerization by Intricate Cellulosomal Systems
2017Cellulosomes are multi-enzymatic nanomachines that have been fine-tuned through evolution to efficiently deconstruct plant biomass. Integration of cellulosomal components occurs via highly ordered protein-protein interactions between the various enzyme-borne dockerin modules and the multiple copies of the cohesin modules located on the scaffoldin ...
Johanna, Stern +4 more
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Cellulosome-like entities inBacteroides cellulosolvens
Current Microbiology, 1991Cellulosome-like complexes were identified in the broth and sonic extracts of cellobiose-and cellulose-grown cells ofBacteroides cellulosolvens. The extracellular fractions contained three to four major polypeptides and several minor polypeptide bands that were localized in two major gel filtration peaks indicating average molecular weights of about ...
Raphael Lamed +3 more
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Cellulosomes from Clostridium thermocellum
1988Publisher Summary Biomass in the form of cellulose is the major constituent of plant matter, thereby comprising the earth's most abundant natural organic resource. Although the repeating unit of this biopolymer is a simple disaccharide (cellobiose), cellulose fibrils are organized into a complicated paracrystalline state.
Raphael Lamed, Edward A. Bayer
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