Results 61 to 70 of about 1,220 (170)

Minimalistic Cellulosome of the Butanologenic Bacterium Clostridium saccharoperbutylacetonicum

mBio, 2020
Clostridium saccharoperbutylacetonicum is a mesophilic, anaerobic, butanol-producing bacterium, originally isolated from soil. It was recently reported that C. saccharoperbutylacetonicum possesses multiple cellulosomal elements and would potentially form
Bosmat Levi Hevroni, Sarah Moraïs, Yonit Ben-David, Ely Morag, Edward A. Bayer   +4 more
doaj   +1 more source

Diversity and Vertical Distribution of Planctomycetota in the Water Column of the Remote North Pacific

Environmental Microbiology Reports, Volume 17, Issue 1, February 2025.
In the remote regions of the Pacific Ocean, Planctomycetota diversity predominantly occupies deeper water layers ranging from 175 to 500 m. Phylogenetic analyses have identified an entire range of previously unknown genera within this group of bacteria.
Inês Rosado Vitorino, Nicola Gambardella, Miguel Semedo, Catarina Magalhães, Olga Maria Lage   +4 more
wiley   +1 more source

Extracytoplasmic polysaccharides control cellulosomal and non-cellulosomal systems in Herbivorax saccincola A7

Applied Microbiology and Biotechnology
Herbivorax saccincola A7 is an anaerobic alkali-thermophilic lignocellulolytic bacterium that possesses a cellulosome and high xylan degradation ability. To understand the expression profile of extracellular enzymes by carbon sources, quantitative real-time PCR was performed on all cellulosomal and non-cellulosomal enzyme genes of H.
Sirilak Baramee, Pakinee Thianheng, Ayaka Uke, Pattsarun Cheawchanlertfa, Chakrit Tachaapaikoon, Rattiya Waeonukul, Patthra Pason, Khanok Ratanakhanokchai, Ya-Jun Liu, Akihiko Kosugi   +9 more
openaire   +2 more sources

A modular enzyme with combined hemicellulose‐removing and LPMO activity increases cellulose accessibility in softwood

The FEBS Journal, Volume 292, Issue 1, Page 75-93, January 2025.
This study describes a unique bi‐catalytic enzyme that combines an oxidative activity (LPMO) acting on cellulose and a hydrolase activity (Man5) acting on mannan thus facilitating the degradation of complex, co‐polymeric plant cell walls. The enzyme works by removing the most abundant cellulose‐covering softwood hemicellulose, mannan, which then allows
Zarah Forsberg, Tina R. Tuveng, Vincent G. H. Eijsink   +2 more
wiley   +1 more source

Comparative characterization of all cellulosomal cellulases from Clostridium thermocellum reveals high diversity in endoglucanase product formation essential for complex activity

Biotechnology for Biofuels, 2017
Background Clostridium thermocellum is a paradigm for efficient cellulose degradation and a promising organism for the production of second generation biofuels. It owes its high degradation rate on cellulosic substrates to the presence of supra-molecular
Benedikt Leis, Claudia Held, Fabian Bergkemper, Katharina Dennemarck, Robert Steinbauer, Alarich Reiter, Matthias Mechelke, Matthias Moerch, Sigrid Graubner, Wolfgang Liebl, Wolfgang H. Schwarz, Vladimir V. Zverlov   +11 more
doaj   +1 more source

Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production

Biotechnology for Biofuels, 2020
Backgrounds Engineering yeast as a consolidated bioprocessing (CBP) microorganism by surface assembly of cellulosomes has been aggressively utilized for cellulosic ethanol production.
Ce Dong, Jie Qiao, Xinping Wang, Wenli Sun, Lixia Chen, Shuntang Li, Ke Wu, Lixin Ma, Yi Liu   +8 more
doaj   +1 more source

Enhancement of Consolidated Bioprocessing for Bioethanol Conversion From Water Hyacinth Using the Wild and Mutant Bacterial Strains

International Journal of Chemical Engineering, Volume 2025, Issue 1, 2025.
Bacterial strains Hangateiclostridium thermocellum KSMK1203 and Lachnoclostridium phytofermentans KSM 1203 were subjected to physiochemical mutagenesis to improve their capacity for cellulose degradation, boost ethanol production, and enhance their resilience against various substrates and byproducts.
Kavitha S., Selvakumar Periyasamy, Gajendran T., Yuvaraj S., Arnab Biswas   +4 more
wiley   +1 more source

A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum.

PLoS ONE, 2013
The composition of the cellulosomes (multi enzymatic complexes involved in the degradation of plant cell wall polysaccharides) produced by Clostridium cellulolyticum differs according to the growth substrate. In particular, the expression of a cluster of
Hamza Celik, Jean-Charles Blouzard, Birgit Voigt, Dörte Becher, Valentine Trotter, Henri-Pierre Fierobe, Chantal Tardif, Sandrine Pagès, Pascale de Philip   +8 more
doaj   +1 more source

Leveraging the Power of Enzymes in Engineered Dead and Living Materials

Advanced Functional Materials, Volume 34, Issue 44, October 29, 2024.
Insights gained during the design of functional enzyme materials have immense potential to supercharge the nascent Engineered Living Material (ELM) field. In this review, these impressive material design strategies are unpacked and contrasted with the enzyme‐control systems used by microorganisms.
Mark R. Shannon, Bini Zhou, Adam W. Perriman   +2 more
wiley   +1 more source

Molecular mechanics studies of factors affecting overall rate in cascade reactions: Multi‐enzyme colocalization and environment

Protein Science, Volume 33, Issue 10, October 2024.
Abstract Millions of years of evolution have optimized many biosynthetic pathways by use of multi‐step catalysis. In addition, multi‐step metabolic pathways are commonly found in and on membrane‐bound organelles in eukaryotic biochemistry. The fundamental mechanisms that facilitate these reaction processes provide strategies to bioengineer metabolic ...
Shivansh Kaushik, Ta I Hung, Chia‐en A. Chang   +2 more
wiley   +1 more source