Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction [PDF]
Scientific Reports, 2018 Cellulosomes are highly sophisticated molecular nanomachines that participate in the deconstruction of complex polysaccharides, notably cellulose and hemicellulose.
Pedro Bule +2 more
exaly +6 more sources
The Trichohyalin-Like Protein Scaffoldin Is Expressed in the Multilayered Periderm during Development of Avian Beak and Egg Tooth [PDF]
Genes, 2021 Scaffoldin, an S100 fused-type protein (SFTP) with high amino acid sequence similarity to the mammalian hair follicle protein trichohyalin, has been identified in reptiles and birds, but its functions are not yet fully understood.
Veronika Mlitz +2 more
exaly +5 more sources
Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum [PDF]
Applied and Environmental Microbiology, 2017 Cellulosomes are considered to be one of the most efficient systems for the degradation of plant cell wall polysaccharides. The central cellulosome component comprises a large, noncatalytic protein subunit called scaffoldin.
Ivan Muñoz-Gutiérrez +2 more
exaly +4 more sources
Cell‐surface exposure of a hybrid 3‐cohesin scaffoldin allowing the functionalization of Escherichia coli envelope [PDF]
Biotechnology and Bioengineering, 2020 International audienceCellulosomes are large plant cell wall degrading complexes secreted by some anaerobic bacteria. They are typically composed of a major scaffolding protein containing multiple receptors called cohesins, which tightly anchor a small ...
Nicolas Vita +2 more
exaly +4 more sources
Solution conformation of a cohesin module and its scaffoldin linker from a prototypical cellulosome
Archives of Biochemistry and Biophysics, 2018 Bacterial cellulases are drawing increased attention as a means to obtain plentiful chemical feedstocks and fuels from renewable lignocellulosic biomass sources. Certain bacteria deploy a large extracellular multi-protein complex, called the cellulosome,
Albert Galera-Prat +2 more
exaly +6 more sources
Role of the CipA Scaffoldin Protein in Cellulose Solubilization, as Determined by Targeted Gene Deletion and Complementation in Clostridium thermocellum [PDF]
Journal of Bacteriology, 2013 The CipA scaffoldin protein plays a key role in the Clostridium thermocellum cellulosome. Previous studies have revealed that mutants deficient in binding or solubilizing cellulose also exhibit reduced expression of CipA. To confirm that CipA is, in fact,
Daniel G Olson +2 more
exaly +5 more sources
Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature [PDF]
mBio, 2016 Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes. The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
Johanna Stern +3 more
doaj +3 more sources
Scaffoldin Conformation and Dynamics Revealed by a Ternary Complex from the Clostridium thermocellum Cellulosome [PDF]
Journal of Biological Chemistry, 2012 Cellulosomes are multienzyme complexes responsible for efficient degradation of plant cell wall polysaccharides. The nonenzymatic scaffoldin subunit provides a platform for cellulolytic enzyme binding that enhances the overall activity of the bound enzymes. Understanding the unique quaternary structural elements responsible for the enzymatic synergy of
Mark A Currie +2 more
exaly +3 more sources
Cell‐surface binding domains from Clostridium cellulovorans can be used for surface display of cellulosomal scaffoldins in Lactococcus lactis [PDF]
Biotechnology Journal, Volume 16, Issue 8, August 2021., 2021 Engineering microbial strains combining efficient lignocellulose metabolization and high‐value chemical production is a cutting‐edge strategy towards cost‐sustainable 2nd generation biorefining. Here, Lactococcus lactis (efficient lactic acid producer) was engineered with synthetic scaffoldins for assembly of secreted and cell‐surface displayed ...
Loredana Tarraran +3 more
wiley +4 more sources
Microbial Biotechnology, 2020 Summary The multi‐enzyme cellulosome complex can mediate the valorization of lignocellulosic biomass into soluble sugars that can serve in the production of biofuels and valuable products.
Yonit Ben‐David +3 more
doaj +2 more sources