Saccharomyces cerevisiae cell surface display technology: Strategies for improvement and applications [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Microbial cell surface display technology provides a powerful platform for engineering proteins/peptides with enhanced properties. Compared to the classical intracellular and extracellular expression (secretion) systems, this technology avoids enzyme ...
Chenmeng Zhang +17 more
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Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study [PDF]
Biotechnology for Biofuels and Bioproducts, 2022 Background Designer cellulosomes are self-assembled chimeric enzyme complexes that can be used to improve lignocellulosic biomass degradation. They are composed of a synthetic multimodular backbone protein, termed the scaffoldin, and a range of different
Julie Vanderstraeten +8 more
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Single-cell analysis of yeast surface display for designer cellulosome applications using a fluorescent protein complex [PDF]
Microbiology SpectrumDesigner cellulosomes (DCs) are precisely engineered multi-enzyme complexes aimed at lignocellulose saccharification. Achieving spontaneous designer cellulosome display on yeast cell surfaces has been a long-term objective to enhance consolidated ...
Babette Lamote +8 more
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Mapping the deformability of natural and designed cellulosomes in solution [PDF]
Biotechnology for Biofuels and Bioproducts, 2022 Background Natural cellulosome multi-enzyme complexes, their components, and engineered ‘designer cellulosomes’ (DCs) promise an efficient means of breaking down cellulosic substrates into valuable biofuel products.
Jonathan Dorival +11 more
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Development-Associated Genes of the Epidermal Differentiation Complex (EDC) [PDF]
Journal of Developmental BiologyThe epidermal differentiation complex (EDC) is a cluster of genes that encode protein components of the outermost layers of the epidermis in mammals, reptiles and birds.
Karin Brigit Holthaus, Leopold Eckhart
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The Epithelial Egg Tooth of the Chicken Shares Protein Markers with the Embryonic Subperiderm and Feathers [PDF]
Journal of Developmental BiologyThe epithelial egg tooth is used by birds to open the eggshell for hatching. This ectodermal structure consists of a multilayered periderm and a hard cornified portion, the caruncle or actual egg tooth.
Attila Placido Sachslehner +4 more
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Establishment of a culture model for the prolonged maintenance of chicken feather follicles structure in vitro. [PDF]
PLoS ONE, 2022 Protocols allowing the in vitro culture of human hair follicles in a serum free-medium up to 9 days were developed 30 years ago. By using similar protocols, we achieved the prolonged maintenance in vitro of juvenile feather follicles (FF) microdissected ...
Corentin Mallet +5 more
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A genomic analysis reveals the diversity of cellulosome displaying bacteria [PDF]
Frontiers in MicrobiologyIntroductionSeveral species of cellulolytic bacteria display cellulosomes, massive multi-cellulase containing complexes that degrade lignocellulosic plant biomass (LCB). A greater understanding of cellulosome structure and enzyme content could facilitate
Christine M. Minor +10 more
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Minimalistic Cellulosome of the Butanologenic Bacterium
mBio, 2020 Clostridium saccharoperbutylacetonicum is a mesophilic, anaerobic, butanol-producing bacterium, originally isolated from soil. It was recently reported that C. saccharoperbutylacetonicum possesses multiple cellulosomal elements and would potentially form
Bosmat Levi Hevroni +4 more
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Cells, 2022 The immunophilin FKBP51, the angiomotin AmotL2, and the scaffoldin IQGAP1 are overexpressed in many types of cancer, with the highest increase in leucocytes from patients undergoing oxaliplatin chemotherapy.
Rebeca González-Fernández +5 more
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