Results 1 to 10 of about 1,083 (143)

Saccharomyces cerevisiae cell surface display technology: Strategies for improvement and applications [PDF]

Frontiers in Bioengineering and Biotechnology, 2022
Microbial cell surface display technology provides a powerful platform for engineering proteins/peptides with enhanced properties. Compared to the classical intracellular and extracellular expression (secretion) systems, this technology avoids enzyme ...
Chenmeng Zhang, Chenmeng Zhang, Chenmeng Zhang, Hongyu Chen, Hongyu Chen, Hongyu Chen, Yiping Zhu, Yiping Zhu, Yiping Zhu, Yu Zhang, Yu Zhang, Yu Zhang, Xun Li, Xun Li, Xun Li, Fei Wang, Fei Wang, Fei Wang   +17 more
doaj   +2 more sources

Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study [PDF]

Biotechnology for Biofuels and Bioproducts, 2022
Background Designer cellulosomes are self-assembled chimeric enzyme complexes that can be used to improve lignocellulosic biomass degradation. They are composed of a synthetic multimodular backbone protein, termed the scaffoldin, and a range of different
Julie Vanderstraeten, Maria João Maurício da Fonseca, Philippe De Groote, Dennis Grimon, Hans Gerstmans, Amaranta Kahn, Sarah Moraïs, Edward A. Bayer, Yves Briers   +8 more
doaj   +2 more sources

Single-cell analysis of yeast surface display for designer cellulosome applications using a fluorescent protein complex [PDF]

Microbiology Spectrum
Designer cellulosomes (DCs) are precisely engineered multi-enzyme complexes aimed at lignocellulose saccharification. Achieving spontaneous designer cellulosome display on yeast cell surfaces has been a long-term objective to enhance consolidated ...
Babette Lamote, Emma Cremelie, Kristel Demeyere, Philippe De Groote, Dennis Grimon, Julie Vanderstraeten, Evelyne Meyer, Marjan De Mey, Yves Briers   +8 more
doaj   +2 more sources

Beyond enzyme engineering: ordered enzyme immobilization drives enhanced productivity in vitro [PDF]

Frontiers in Bioengineering and Biotechnology
Increasing environmental and economic pressures associated with global fossil fuel demand necessitate a shift toward sustainable fuel production. Production of second-generation biofuels, such as isobutanol, presents a promising opportunity; however ...
Matthew Wong, Thomas G. Neuman, Md Anarul Hoque, Sarah Moraïs, Edward Bayer, Edward Bayer, Georges Belfort, Mattheos Koffas   +7 more
doaj   +2 more sources

Mapping the deformability of natural and designed cellulosomes in solution [PDF]

Biotechnology for Biofuels and Bioproducts, 2022
Background Natural cellulosome multi-enzyme complexes, their components, and engineered ‘designer cellulosomes’ (DCs) promise an efficient means of breaking down cellulosic substrates into valuable biofuel products.
Jonathan Dorival, Sarah Moraïs, Aurore Labourel, Bartosz Rozycki, Pierre-Andre Cazade, Jérôme Dabin, Eva Setter-Lamed, Itzhak Mizrahi, Damien Thompson, Aurélien Thureau, Edward A. Bayer, Mirjam Czjzek   +11 more
doaj   +2 more sources

Development-Associated Genes of the Epidermal Differentiation Complex (EDC) [PDF]

Journal of Developmental Biology
The epidermal differentiation complex (EDC) is a cluster of genes that encode protein components of the outermost layers of the epidermis in mammals, reptiles and birds.
Karin Brigit Holthaus, Leopold Eckhart
doaj   +2 more sources

Rapid adaptation for fibre degradation by changes in plasmid stoichiometry within Lactobacillus plantarum at the synthetic community level

Microbial Biotechnology, 2020
Summary The multi‐enzyme cellulosome complex can mediate the valorization of lignocellulosic biomass into soluble sugars that can serve in the production of biofuels and valuable products.
Yonit Ben‐David, Sarah Moraïs, Edward A. Bayer, Itzhak Mizrahi   +3 more
doaj   +2 more sources

A Scaffoldomics Platform for Modular In Vivo Enzyme Colocalisation and Its Application to Naringenin Biosynthesis [PDF]

Microbial Biotechnology, Volume 19, Issue 3, March 2026.
A hierarchical assembly system was established in which the coding sequences for four docking enzymes and a scaffold are co‐assembled onto a single plasmid, thereby establishing a ‘scaffoldomics’ platform for the optimisation of multi‐enzyme biosynthetic pathways.
Marte Elias, Brecht De Paepe, Julie Vanderstraeten, Babette Lamote, Marjan De Mey, Yves Briers   +5 more
wiley   +2 more sources

Establishment of a culture model for the prolonged maintenance of chicken feather follicles structure in vitro. [PDF]

PLoS ONE, 2022
Protocols allowing the in vitro culture of human hair follicles in a serum free-medium up to 9 days were developed 30 years ago. By using similar protocols, we achieved the prolonged maintenance in vitro of juvenile feather follicles (FF) microdissected ...
Corentin Mallet, Laurent Souci, Mireille Ledevin, Sonia Georgeault, Thibaut Larcher, Caroline Denesvre   +5 more
doaj   +2 more sources

The Epithelial Egg Tooth of the Chicken Shares Protein Markers with the Embryonic Subperiderm and Feathers [PDF]

Journal of Developmental Biology
The epithelial egg tooth is used by birds to open the eggshell for hatching. This ectodermal structure consists of a multilayered periderm and a hard cornified portion, the caruncle or actual egg tooth.
Attila Placido Sachslehner, Julia Steinbinder, Claudia Hess, Veronika Mlitz, Leopold Eckhart   +4 more
doaj   +2 more sources