Structural and functional insights into cellulosomes: masters of plant cell wall degradation [PDF]
Frontiers in MicrobiologyCellulosomes are complex multi-enzyme systems that enable efficient cellulose breakdown in some anaerobic bacteria and fungi. Understanding cellulosome functionality plays a crucial role in expanding their potential for industrial plant biomass ...
Nataša Lindič, Maša Vodovnik
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Functional characterization of a dockerin-containing expansin-like protein from the anaerobic fungus Neocallimastix californiae [PDF]
Biotechnology for Biofuels and BioproductsBackground Anaerobic microbes produce multienzyme complexes known as cellulosomes to enhance the degradation of cellulosic substrates. These complexes localize diverse enzymes onto a protein scaffold, where proteins are anchored by dockerin domains ...
Taru Koitto +5 more
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A genomic analysis reveals the diversity of cellulosome displaying bacteria [PDF]
Frontiers in MicrobiologyIntroductionSeveral species of cellulolytic bacteria display cellulosomes, massive multi-cellulase containing complexes that degrade lignocellulosic plant biomass (LCB). A greater understanding of cellulosome structure and enzyme content could facilitate
Christine M. Minor +10 more
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In vitro assembly of the trehalose bi-enzyme complex with artificial scaffold protein [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Introduction: Trehalose is a significant rare sugar known for its stable properties and ability to protect biomolecules from environmental factors.Methods: In this study, we present a novel approach utilizing a scaffold protein-mediated assembly method ...
Xiangyi Wang +13 more
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Limousia bacteria encode mucinolysome for mucin utilization in animal gut microbiomes [PDF]
Gut MicrobesMucins create a physical barrier that protects human and animal tissues from microbial pathogens. Here, we provide evidence that mucin degradation can be mediated by unique mucinolysomes, defined as extracellular cellulosome-like multi-enzyme complexes ...
Jerry Elorm Akresi +8 more
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HLA-DM co-expression enhances MHC class II function in the magnetosome display system [PDF]
Microbiology SpectrumEfficient identification of antigenic peptides that bind to major histocompatibility complex class II (MHC II) molecules is crucial for vaccine and therapeutic development.
Ryoto Tomoe +3 more
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Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome‐Based Synthetic Protein Scaffold [PDF]
Microbial BiotechnologyIn systems biocatalysis, combining pathway enzymes in vitro allows for the conversion of basic substrates into more complex, valuable chemicals. However, in vitro enzyme cascades are not yet economically viable for large‐scale bio‐based chemical ...
Marte Elias +4 more
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Dissection of an Anaerobic Fungal Cellulosomal Endoglucanase: Impact of the Dockerin Module on Activity, Thermostability, and Flexibility [PDF]
ACS OmegaViviane Brito Andrade +9 more
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Frontiers in Bioengineering and Biotechnology, 2023 Introduction: β-Alanine is the only β-amino acid in nature; it is widely used in food additives, medicines, health products, and surfactants. To avoid pollution caused by traditional production methods, the synthesis of β-alanine has been gradually ...
Jie Lu +19 more
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Affinity-induced covalent protein-protein ligation via the SpyCatcher-SpyTag interaction
Green Carbon, 2023 Production of economically viable bioethanol is potentially an environmentally and financially worthwhile endeavor. One major source for fermentable sugars is lignocellulose. However, lignocellulosic biomass is difficult to degrade, owing to its inherent
Jacob O. Fierer +6 more
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