Results 1 to 10 of about 1,461 (191)

A Scaffoldomics Platform for Modular In Vivo Enzyme Colocalisation and Its Application to Naringenin Biosynthesis. [PDF]

Microb Biotechnol
A hierarchical assembly system was established in which the coding sequences for four docking enzymes and a scaffold are co‐assembled onto a single plasmid, thereby establishing a ‘scaffoldomics’ platform for the optimisation of multi‐enzyme biosynthetic pathways.
Elias M, De Paepe B, Vanderstraeten J, Lamote B, De Mey M, Briers Y.   +5 more
europepmc   +2 more sources

Structure and enzymatic characterization of CelD endoglucanase from the anaerobic fungus Piromyces finnis. [PDF]

Appl Microbiol Biotechnol, 2023
Dementiev A, Lillington SP, Jin S, Kim Y, Jedrzejczak R, Michalska K, Joachimiak A, O'Malley MA.   +7 more
europepmc   +1 more source

Deconstruction by <i>C. thermocellum</i>-from microbe mediated to dynamic redistribution of cellulosomes. [PDF]

Life Sci Alliance
Yarbrough JM, Hengge NN, Xu Q, Ziegler SJ, Chung D, Huang S, Moraïs S, Mizrahi I, Bayer EA, Bomble YJ.   +9 more
europepmc   +1 more source

Research progress of multi-enzyme complexes based on the design of scaffold protein. [PDF]

Bioresour Bioprocess, 2023
Wang X, Jiang Y, Liu H, Yuan H, Huang D, Wang T.   +5 more
europepmc   +1 more source

Plastic-binding peptides as anchors for protein scaffolds on synthetic plastics: opportunities and challenges. [PDF]

RSC Adv
Retnadhas S, Hegg EL, Ducat DC.
europepmc   +1 more source

Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum

Cellulosomes, synthesized by anaerobic microorganisms such as Clostridium thermocellum, are remarkably complex nanomachines that efficiently degrade plant cell wall polysaccharides.
Fontes CMGA, Gilbert HJ, Prates JAM, Alves VD, Bolam DN, Ferreira LMA, Fernandes VO, Pinheiro BA, Sakka K   +8 more
core  

Spatial constraints drive amylosome-mediated resistant starch degradation by Ruminococcus bromii in the human colon. [PDF]

Nat Commun
Wimmer BH, Moraïs S, Amit I, Tovar-Herrera O, Tatli M, Trautwein-Schult A, Pfister B, Zalk R, Tödtli P, Simoni S, Lisibach M, Levin L, Becher D, Bayer EA, Medalia O, Mizrahi I.   +15 more
europepmc   +1 more source

Insights into lignocellulose degradation: comparative genomics of anaerobic and cellulolytic Ruminiclostridium-type species. [PDF]

Front Microbiol, 2023
You M, Zhao Q, Liu Y, Zhang W, Shen Z, Ren Z, Xu C.   +6 more
europepmc   +1 more source

Novel Clostridium thermocellum Type I Cohesin-Dockerin Complexes Reveal a Single Binding Mode

Protein-protein interactions play a pivotal role in a large number of biological processes exemplified by the assembly of the cellulosome. Integration of cellulosomal components occurs through the binding of type I cohesin modules located in a non ...
Carvalho AL, Romao MJ, Fontes CMGA, Gilbert HJ, Najmudin S, Prates JAM, Alves VD, Bolam DN, Ferreira LMA, Bras JLA   +9 more
core  

Bio-inspired designer cellulosomes show strongest synergy on industrial substrates under natural-like conditions. [PDF]

J Ind Microbiol Biotechnol
Lamote B, Vanderstraeten J, De Ridder N, Meert K, Boerjan W, Briers Y.   +5 more
europepmc   +1 more source