Results 1 to 10 of about 139 (126)

Constructing an Efficient Bacillus subtilis Spore Display by Using Cohesin−Dockerin Interactions [PDF]

Molecules, 2021
Bacillus subtilis spore display has become a field of increasing interest in the past two decades. To improve the efficiency of B. subtilis spore display, its directed modification was performed based on the cellulosome architecture by introducing onto ...
He Wang, Xiaomin Jiang, Yongchang Qian, Lianghong Yin   +3 more
doaj   +3 more sources

Evidence for a dual binding mode of dockerin modules to cohesins [PDF]

Proceedings of the National Academy of Sciences of the United States of America, 2007
The assembly of proteins that display complementary activities into macromolecular complexes is critical to cellular function. One such enzyme complex, of environmental significance, is the plant cell wall degrading apparatus of anaerobic bacteria, termed the cellulosome.
Edward Bayer, CARLOS M G A Fontes, Maria João Romão   +2 more
exaly   +4 more sources

Cohesin-dockerin interaction in cellulosome assembly: a single Asp-to-Asn mutation disrupts high-affinity cohesin-dockerin binding

FEBS Letters, 2004
The cohesive cellulosome complex is sustained by the high‐affinity cohesin–dockerin interaction. In previous work [J. Biol. Chem. 276 (2001) 9883], we demonstrated that a single Thr‐to‐Leu replacement in the Clostridium thermocellum dockerin component differentiates between non‐recognition and high‐affinity recognition by the interspecies rival cohesin
Yuval Shoham
exaly   +3 more sources

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes [PDF]

Scientific Reports, 2017
Abtract Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates.
Pedro Bule, Victor D. Alves, Vered Israeli-Ruimy, Ana L. Carvalho, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Shabir Najmudin, Edward A. Bayer, Carlos M. G. A. Fontes   +9 more
doaj   +5 more sources

Dissection of an Anaerobic Fungal Cellulosomal Endoglucanase: Impact of the Dockerin Module on Activity, Thermostability, and Flexibility

ACS Omega
Cellulosomal cellulases possess an extra noncatalytic module denominated dockerin, which interacts with the scaffolding via cohesion modules to organize the enzymes within the cellulosome. Given the lack of previously solved experimental atomic structures for modular anaerobic fungal cellulases containing dockerin modules, here we employed structural ...
Viviane Brito Andrade, Robson Tramontina, Dnane Vieira Almeida, Geizecler Tomazetto, Viviam M. da Silva, Frank Gabel, Yolanda M.B. Marcello, Ana Ligia Scott, Fabio Marcio Squina, Wanius Garcia   +9 more
doaj   +4 more sources

Analysis of cohesin-dockerin interactions using mutant dockerin proteins [PDF]

FEMS Microbiology Letters, 2010
Clostridial cellulosomes are cellulolytic complexes that are formed by highly specific interactions between one of the repeated cohesin modules present in the scaffolding protein and a dockerin module of the catalytic components. Although Clostridium thermocellum Xyn11A dockerin has a typical C.
Kazutaka Sakka, Yuka Sugihara, Sadanari Jindou, Makiko Sakka, Minoru Inagaki, Kazuo Sakka, Tetsuya Kimura   +6 more
openaire   +2 more sources

Cohesin-Dockerin Interaction in Cellulosome Assembly [PDF]

Journal of Biological Chemistry, 2001
The assembly of enzyme components into the cellulosome complex is dictated by the cohesin-dockerin interaction. In a recent article (Mechaly, A., Yaron, S., Lamed, R., Fierobe, H.-P., Belaich, A., Belaich, J.-P., Shoham, Y., and Bayer, E. A. (2000) Proteins 39, 170-177), we provided experimental evidence that four previously predicted dockerin residues
Adva Mechaly, Henri-Pierre Fierobe, Anne Belaich, Jean-Pierre Belaich, Raphael Lamed, Yuval Shoham, Edward A. Bayer   +6 more
openaire   +4 more sources

Study on the construction technology of β-alanine synthesizing Escherichia coli based on cellulosome assembly

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: β-Alanine is the only β-amino acid in nature; it is widely used in food additives, medicines, health products, and surfactants. To avoid pollution caused by traditional production methods, the synthesis of β-alanine has been gradually ...
Jie Lu, Jie Lu, Guodong Wang, Guodong Wang, Cuiping Yang, Cuiping Yang, Zehao Peng, Zehao Peng, Lu Yang, Lu Yang, Bowen Du, Bowen Du, Chuanzhuang Guo, Songsen Sui, Jianbin Wang, Junlin Li, Ruiming Wang, Ruiming Wang, Junqing Wang, Junqing Wang   +19 more
doaj   +1 more source

Dual binding in cohesin-dockerin complexes: the energy landscape and the role of short, terminal segments of the dockerin module [PDF]

Scientific Reports, 2018
AbstractThe assembly of the polysaccharide degradating cellulosome machinery is mediated by tight binding between cohesin and dockerin domains. We have used an empirical model known as FoldX as well as molecular mechanics methods to determine the free energy of binding between a cohesin and a dockerin from Clostridium thermocellum in two possible modes
Michał Wojciechowski, Bartosz Różycki, Pham Dinh Quoc Huy, Mai Suan Li, Edward A. Bayer, Marek Cieplak   +5 more
openaire   +2 more sources

Affinity-induced covalent protein-protein ligation via the SpyCatcher-SpyTag interaction

Green Carbon, 2023
Production of economically viable bioethanol is potentially an environmentally and financially worthwhile endeavor. One major source for fermentable sugars is lignocellulose. However, lignocellulosic biomass is difficult to degrade, owing to its inherent
Jacob O. Fierer, Omar E. Tovar-Herrera, Jonathan Y. Weinstein, Amaranta Kahn, Sarah Moraïs, Itzhak Mizrahi, Edward A. Bayer   +6 more
doaj   +1 more source