Constructing an Efficient Bacillus subtilis Spore Display by Using Cohesin−Dockerin Interactions [PDF]
Molecules, 2021 Bacillus subtilis spore display has become a field of increasing interest in the past two decades. To improve the efficiency of B. subtilis spore display, its directed modification was performed based on the cellulosome architecture by introducing onto ...
He Wang +3 more
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In vitro assembly of the trehalose bi-enzyme complex with artificial scaffold protein [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Introduction: Trehalose is a significant rare sugar known for its stable properties and ability to protect biomolecules from environmental factors.Methods: In this study, we present a novel approach utilizing a scaffold protein-mediated assembly method ...
Xiangyi Wang +13 more
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Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex [PDF]
Proceedings of the National Academy of Sciences, 2003 The utilization of organized supramolecular assemblies to exploit the synergistic interactions afforded by close proximity, both for enzymatic synthesis and for the degradation of recalcitrant substrates, is an emerging theme in cellular biology ...
Davies, Gideon J. +8 more
core +4 more sources
Limousia bacteria encode mucinolysome for mucin utilization in animal gut microbiomes [PDF]
Gut MicrobesMucins create a physical barrier that protects human and animal tissues from microbial pathogens. Here, we provide evidence that mucin degradation can be mediated by unique mucinolysomes, defined as extracellular cellulosome-like multi-enzyme complexes ...
Jerry Elorm Akresi +8 more
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Journal of Structural Biology, 2014 Dockerin modules of the cellulosomal enzyme subunits play an important role in the assembly of the cellulosome by binding tenaciously to cohesin modules of the scaffoldin subunit.
Chao Chen, Zhenling Cui, Qiu Cui
exaly +2 more sources
HLA-DM co-expression enhances MHC class II function in the magnetosome display system [PDF]
Microbiology SpectrumEfficient identification of antigenic peptides that bind to major histocompatibility complex class II (MHC II) molecules is crucial for vaccine and therapeutic development.
Ryoto Tomoe +3 more
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Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes [PDF]
Scientific Reports, 2017 Abtract Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates.
Pedro Bule +9 more
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Microbial Cell Factories, 2012 Background The microbial synthesis of fuels, commodity chemicals, and bioactive compounds necessitates the assemblage of multiple enzyme activities to carry out sequential chemical reactions, often via substrate channeling by means of multi-domain or ...
Wieczorek Andrew S, Martin Vincent JJ
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Resolving dual binding conformations of cellulosome cohesin-dockerin complexes using single-molecule force spectroscopy [PDF]
eLife, 2015 Receptor-ligand pairs are ordinarily thought to interact through a lock and key mechanism, where a unique molecular conformation is formed upon binding.
Markus A Jobst +7 more
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Single versus dual-binding conformations in cellulosomal cohesin–dockerin complexes
Current Opinion in Structural Biology, 2016 Cohesins and dockerins are complementary interacting protein modules that form stable and highly specific receptor-ligand complexes. They play a crucial role in the assembly of cellulose-degrading multi-enzyme complexes called cellulosomes and have ...
Bayer, Edward A. +3 more
core +6 more sources