Results 31 to 40 of about 139 (126)

Tripartite binding mode of cohesin-dockerin complexes from Ruminococcus flavefaciens involving naturally truncated dockerins

Journal of Biological Chemistry
Polysaccharides in plant cell walls serve as a rich carbon and energy source, yet their structural complexity presents a barrier to efficient degradation. To address this, anaerobic microorganisms like R. flavefaciens have developed sophisticated multi-enzyme complexes known as cellulosomes, which enable the efficient breakdown of these recalcitrant ...
Marlene Duarte, Ana Luísa Carvalho, Magda C. Ferreira, Beatriz Caires, Maria João Romão, José A.M. Prates, Shabir Najmudin, Edward A. Bayer, Carlos MGA. Fontes, Pedro Bule   +9 more
openaire   +4 more sources

In vitro assembly of the trehalose bi-enzyme complex with artificial scaffold protein

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: Trehalose is a significant rare sugar known for its stable properties and ability to protect biomolecules from environmental factors.Methods: In this study, we present a novel approach utilizing a scaffold protein-mediated assembly method ...
Xiangyi Wang, Xiangyi Wang, Yi Jiang, Yi Jiang, Hongling Liu, Hongling Liu, Xinyi Zhang, Xinyi Zhang, Haibo Yuan, Haibo Yuan, Di Huang, Di Huang, Tengfei Wang, Tengfei Wang   +13 more
doaj   +1 more source

Unique Organization of Extracellular Amylases into Amylosomes in the Resistant Starch-Utilizing Human Colonic Firmicutes Bacterium Ruminococcus bromii

mBio, 2015
Ruminococcus bromii is a dominant member of the human gut microbiota that plays a key role in releasing energy from dietary starches that escape digestion by host enzymes via its exceptional activity against particulate “resistant” starches.
Xiaolei Ze, Yonit Ben David, Jenny A. Laverde-Gomez, Bareket Dassa, Paul O. Sheridan, Sylvia H. Duncan, Petra Louis, Bernard Henrissat, Nathalie Juge, Nicole M. Koropatkin, Edward A. Bayer, Harry J. Flint   +11 more
doaj   +1 more source

Novel Organization and Divergent Dockerin Specificities in the Cellulosome System of Ruminococcus flavefaciens [PDF]

Journal of Bacteriology, 2003
ABSTRACT The DNA sequence coding for putative cellulosomal scaffolding protein ScaA from the rumen cellulolytic anaerobe Ruminococcus flavefaciens 17 was completed. The mature protein exhibits a calculated molecular mass of 90,198 Da and comprises three cohesin domains, a C-terminal dockerin, and a unique ...
Rincon M.T.1, Ding S.Y.2, McCrae S.I.1, Martin J.C.1, Aurilia V.3, Lamed, R.4, Shoham, Y.5, Bayer E.A.2, Flint H.J.1   +10 more
openaire   +2 more sources

Effects of synthetic cohesin-containing scaffold protein architecture on binding dockerin-enzyme fusions on the surface of Lactococcus lactis

Microbial Cell Factories, 2012
Background The microbial synthesis of fuels, commodity chemicals, and bioactive compounds necessitates the assemblage of multiple enzyme activities to carry out sequential chemical reactions, often via substrate channeling by means of multi-domain or ...
Wieczorek Andrew S, Martin Vincent JJ
doaj   +1 more source

Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase

Microbial Cell Factories, 2013
Background Cellulosic biomass is considered as a promising alternative to fossil fuels, but its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this material.
Kim Sujin, Baek Seung-Ho, Lee Kyusung, Hahn Ji-Sook   +3 more
doaj   +1 more source

Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature

mBio, 2016
Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes. The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
Johanna Stern, Sarah Moraïs, Raphael Lamed, Edward A. Bayer   +3 more
doaj   +1 more source

Improving the Synthesis Efficiency of Amino Acids Such as L-Lysine by Assembling Artificial Cellulosome Elements Dockerin Protein In Vivo

Fermentation, 2022
Cellulosome is a highly efficient multi-enzyme self-assembly system and is found on the extracellular surface or in the free environment of microorganisms. However, with a lack of Ca2+ in vivo, cellulosome assembly is challenging.
Nan Li, Le Xue, Zirui Wang, Peng Du, Piwu Li, Jing Su, Jing Xiao, Min Wang, Junqing Wang, Ruiming Wang   +9 more
doaj   +1 more source

The Clostridium cellulolyticum Dockerin Displays a Dual Binding Mode for Its Cohesin Partner [PDF]

Journal of Biological Chemistry, 2008
The plant cell wall degrading apparatus of anaerobic bacteria includes a large multienzyme complex termed the "cellulosome." The complex assembles through the interaction of enzyme-derived dockerin modules with the multiple cohesin modules of the noncatalytic scaffolding protein.
Pinheiro BA, Proctor MR, Martinez-Fleites C, Prates JA, Money VA, Davies GJ, Bayer EA, Fontesm CM, Fierobe HP, Gilbert HJ   +9 more
openaire   +3 more sources

A genomic analysis reveals the diversity of cellulosome displaying bacteria

Frontiers in Microbiology
IntroductionSeveral species of cellulolytic bacteria display cellulosomes, massive multi-cellulase containing complexes that degrade lignocellulosic plant biomass (LCB). A greater understanding of cellulosome structure and enzyme content could facilitate
Christine M. Minor, Christine M. Minor, Allen Takayesu, Allen Takayesu, Sung Min Ha, Lukasz Salwinski, Michael R. Sawaya, Matteo Pellegrini, Robert T. Clubb, Robert T. Clubb, Robert T. Clubb   +10 more
doaj   +1 more source