Results 31 to 40 of about 1,595 (147)

Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature

mBio, 2016
Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes. The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
Johanna Stern, Sarah Moraïs, Raphael Lamed, Edward A. Bayer   +3 more
doaj   +1 more source

Improving the Synthesis Efficiency of Amino Acids Such as L-Lysine by Assembling Artificial Cellulosome Elements Dockerin Protein In Vivo

Fermentation, 2022
Cellulosome is a highly efficient multi-enzyme self-assembly system and is found on the extracellular surface or in the free environment of microorganisms. However, with a lack of Ca2+ in vivo, cellulosome assembly is challenging.
Nan Li, Le Xue, Zirui Wang, Peng Du, Piwu Li, Jing Su, Jing Xiao, Min Wang, Junqing Wang, Ruiming Wang   +9 more
doaj   +1 more source

Mapping the deformability of natural and designed cellulosomes in solution

Biotechnology for Biofuels and Bioproducts, 2022
Background Natural cellulosome multi-enzyme complexes, their components, and engineered ‘designer cellulosomes’ (DCs) promise an efficient means of breaking down cellulosic substrates into valuable biofuel products.
Jonathan Dorival, Sarah Moraïs, Aurore Labourel, Bartosz Rozycki, Pierre-Andre Cazade, Jérôme Dabin, Eva Setter-Lamed, Itzhak Mizrahi, Damien Thompson, Aurélien Thureau, Edward A. Bayer, Mirjam Czjzek   +11 more
doaj   +1 more source

Evidence for a dual binding mode of dockerin modules to cohesins

The assembly of proteins that display complementary activities into macromolecular complexes is critical to cellular function. One such enzyme complex, of environmental significance, is the plant cell wall degrading apparatus of anaerobic bacteria ...
Carvalho AL, Romao MJ, Fontes CMGA, Smith N, Gilbert HJ, Prates JAM, Nagy T, Davies GJ, Bayer EA, Ferreira LMA, Dias FMV, Proctor MR   +11 more
core   +5 more sources

Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex

The utilization of organized supramolecular assemblies to exploit the synergistic interactions afforded by close proximity, both for enzymatic synthesis and for the degradation of recalcitrant substrates, is an emerging theme in cellular biology ...
Carvalho AL, Romao MJ, Fontes CMGA, Gilbert HJ, Prates JAM, Nagy T, Davies GJ, Ferreira LMA, Dias FMV   +8 more
core   +5 more sources

CRISPR-PIN: Modifying gene position in the nucleus via dCas9-mediated tethering

Synthetic and Systems Biotechnology, 2019
Spatial organization of DNA within the nucleus is important for controlling DNA replication and repair, genetic recombination, and gene expression. Here, we present CRISPR-PIN, a CRISPR/dCas9-based tool that allows control of gene Position in the Nucleus
Jyun-Liang Lin, Holly Ekas, Matthew Deaner, Hal S. Alper   +3 more
doaj   +1 more source

Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake

Frontiers in Microbiology, 2021
The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential.
Timothy J. Williams, Michelle A. Allen, Jonathan F. Berengut, Ricardo Cavicchioli   +3 more
doaj   +1 more source

Dual binding in cohesin-dockerin complexes: the energy landscape and the role of short, terminal segments of the dockerin module

, 2018
The assembly of the polysaccharide degradating cellulosome machinery is mediated by tight binding between cohesin and dockerin domains. We have used an empirical model known as FoldX as well as molecular mechanics methods to determine the free energy of ...
Michał Wojciechowski, Bartosz Różycki, Edward A. Bayer, Pham Dinh Quoc Huy, Marek Cieplak, Mai Suan Li   +5 more
core   +1 more source

Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

BMC Genomics, 2012
Background Microbial degradation of plant cell walls and its conversion to sugars and other byproducts is a key step in the carbon cycle on Earth. In order to process heterogeneous plant-derived biomass, specialized anaerobic bacteria use an elaborate ...
Dassa Bareket, Borovok Ilya, Lamed Raphael, Henrissat Bernard, Coutinho Pedro, Hemme Christopher L, Huang Yue, Zhou Jizhong, Bayer Edward A   +8 more
doaj   +1 more source

Silanes to Silatranes: Robust Functionalization for Single‐Molecule Force Spectroscopy

ChemBioChem, Volume 27, Issue 9, 14 May 2026.
3‐(aminopropyl)silatrane (APS) has superior stability for surface functionalization in single‐molecule force spectroscopy experiments compared to conventional silane‐based methods. This paper utilizes bacterial adhesin attachment to demonstrate the advantages of APS‐based surface functionalization in single‐molecule unfolding experiments of a model ...
Thomas D. Courtney, Christopher B. Hatchell, Xuliana O, David R. Jacobson   +3 more
wiley   +1 more source