Results 51 to 60 of about 1,461 (191)

The Cellulosome Paradigm in An Extreme Alkaline Environment

Microorganisms, 2019
Rapid decomposition of plant biomass in soda lakes is associated with microbial activity of anaerobic cellulose-degrading communities. The alkaliphilic bacterium, Clostridium alkalicellulosi, is the single known isolate from a soda lake that demonstrates
Paripok Phitsuwan, Sarah Moraïs, Bareket Dassa, Bernard Henrissat, Edward A. Bayer   +4 more
doaj   +1 more source

The secretome of Thermococcus barophilus in the presence of carbohydrates and the potential role of the TrmBL4 regulator

Environmental Microbiology Reports, Volume 15, Issue 6, Page 530-544, December 2023., 2023
The secretome of Thermococcus barophilus was explored in the light of sugar metabolism (maltose, maltodextrin and pectin). The expression of certain secreted proteins is directly dependent on the sugar regulator TrmBL4, as is the ABC maltose/maltodextrin transporter (MD system), but not the trehalose/maltose transport system (TM system).
Maria Batour, Sébastien Laurent, Yann Moalic, Hala Chamieh, Samir Taha, Mohamed Jebbar   +5 more
wiley   +1 more source

Minimalistic Cellulosome of the Butanologenic Bacterium Clostridium saccharoperbutylacetonicum

mBio, 2020
Clostridium saccharoperbutylacetonicum is a mesophilic, anaerobic, butanol-producing bacterium, originally isolated from soil. It was recently reported that C. saccharoperbutylacetonicum possesses multiple cellulosomal elements and would potentially form
Bosmat Levi Hevroni, Sarah Moraïs, Yonit Ben-David, Ely Morag, Edward A. Bayer   +4 more
doaj   +1 more source

Direct Comparison of Lysine versus Site‐Specific Protein Surface Immobilization in Single‐Molecule Mechanical Assays**

Angewandte Chemie, Volume 135, Issue 32, August 7, 2023., 2023
The surface immobilization technique for atomic force microscopy/single‐molecule force spectroscopy is crucial for experimental yield, accuracy and resolution. Reported herein is that a commonly used lysine‐based immobilization can result in significant signal deterioration, including lowering of rupture forces, effective shortening of linkers, and ...
Haipei Liu, Zhaowei Liu, Mariana Sá Santos, Michael A. Nash   +3 more
wiley   +1 more source

Abundance and diversity of dockerin-containing proteins in the fiber-degrading rumen bacterium, Ruminococcus flavefaciens FD-1.

PLoS ONE, 2010
BackgroundThe cellulosome is a multi-enzyme machine, which plays a key role in the breakdown of plant cell walls in many anaerobic cellulose-degrading microorganisms. Ruminococcus flavefaciens FD-1, a major fiber-degrading bacterium present in the gut of
Marco T Rincon, Bareket Dassa, Harry J Flint, Anthony J Travis, Sadanari Jindou, Ilya Borovok, Raphael Lamed, Edward A Bayer, Bernard Henrissat, Pedro M Coutinho, Dion A Antonopoulos, Margret E Berg Miller, Bryan A White   +12 more
doaj   +1 more source

Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system

Biotechnology for Biofuels, 2017
Background (Pseudo) Bacteroides cellulosolvens is an anaerobic, mesophilic, cellulolytic, cellulosome-producing clostridial bacterium capable of utilizing cellulose and cellobiose as carbon sources. Recently, we sequenced the B.
Olga Zhivin, Bareket Dassa, Sarah Moraïs, Sagar M. Utturkar, Steven D. Brown, Bernard Henrissat, Raphael Lamed, Edward A. Bayer   +7 more
doaj   +1 more source

Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme

Microorganisms, 2017
The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides.
Bareket Dassa, Ilya Borovok, Vincent Lombard, Bernard Henrissat, Raphael Lamed, Edward A. Bayer, Sarah Moraïs   +6 more
doaj   +1 more source

Diversity and strain specificity of plant cell wall degrading enzymes revealed by the draft genome of Ruminococcus flavefaciens FD-1. [PDF]

PLoS ONE, 2009
BACKGROUND:Ruminococcus flavefaciens is a predominant cellulolytic rumen bacterium, which forms a multi-enzyme cellulosome complex that could play an integral role in the ability of this bacterium to degrade plant cell wall polysaccharides.
Margret E Berg Miller, Dionysios A Antonopoulos, Marco T Rincon, Mark Band, Albert Bari, Tatsiana Akraiko, Alvaro Hernandez, Jyothi Thimmapuram, Bernard Henrissat, Pedro M Coutinho, Ilya Borovok, Sadanari Jindou, Raphael Lamed, Harry J Flint, Edward A Bayer, Bryan A White   +15 more
doaj   +1 more source

Involvement of Both Dockerin Subdomains in Assembly of the Clostridium thermocellum Cellulosome [PDF]

Journal of Bacteriology, 1998
ABSTRACT Clostridium thermocellum produces an extracellular cellulase complex termed the cellulosome. It consists of a scaffolding protein, CipA, containing nine cohesin domains and a cellulose-binding domain, and at least 14 different enzymatic subunits, each containing a conserved duplicated sequence, or dockerin ...
B, Lytle, J H, Wu
openaire   +2 more sources

Protein scaffolds: A tool for multi-enzyme assembly

Biotechnology Reports, 2021
The synthesis of complex molecules using multiple enzymes simultaneously in one reaction vessel has rapidly emerged as a new frontier in the field of bioprocess technology.
Shubhada Gad, Sonal Ayakar
doaj   +1 more source