Results 71 to 80 of about 139 (126)

Intramolecular clasp of the cellulosomal Ruminococcus flavefaciens ScaA dockerin module confers structural stability [PDF]

FEBS Open Bio, 2013
The cellulosome is a large extracellular multi‐enzyme complex that facilitates the efficient hydrolysis and degradation of crystalline cellulosic substrates. During the course of our studies on the cellulosome of the rumen bacterium Ruminococcus flavefaciens, we focused on the critical ScaA dockerin (ScaADoc), the unique dockerin that incorporates the ...
Slutzki, Michal, Jobby, Maroor K., Chitayat, Seth, Karpol, Alon, Dassa, Bareket, Barak, Yoav, Lamed, Raphael, Smith, Steven P., Bayer, Edward A.   +8 more
openaire   +2 more sources

Structural and molecular insights into a bifunctional glycoside hydrolase 30 xylanase specific to glucuronoxylan

Biotechnology and Bioengineering, Volume 121, Issue 7, Page 2067-2078, July 2024.
Abstract Glycoside hydrolase (GH) 30 family xylanases are enzymes of biotechnological interest due to their capacity to degrade recalcitrant hemicelluloses, such as glucuronoxylan (GX). This study focuses on a subfamily 7 GH30, TtXyn30A from Thermothelomyces thermophilus, which acts on GX in an “endo” and “exo” mode, releasing methyl‐glucuronic acid ...
Christina Pentari, Christos Kosinas, Efstratios Nikolaivits, Maria Dimarogona, Evangelos Topakas   +4 more
wiley   +1 more source

Comparative analysis of carbohydrate active enzymes in Clostridium termitidis CT1112 reveals complex carbohydrate degradation ability.

PLoS ONE, 2014
Clostridium termitidis strain CT1112 is an anaerobic, gram positive, mesophilic, cellulolytic bacillus isolated from the gut of the wood-feeding termite, Nasutitermes lujae.
Riffat I Munir, John Schellenberg, Bernard Henrissat, Tobin J Verbeke, Richard Sparling, David B Levin   +5 more
doaj   +1 more source

Clostridium butyricum and carbohydrate active enzymes contribute to the reduced fat deposition in pigs

iMeta, Volume 3, Issue 1, February 2024.
In the present study, we found significant differences in microbial composition and potential functional capacity among different gut locations in Jinhua pigs with distinct fatness phenotypes. Second, we identified that Jinhua pigs with lower fatness exhibited higher levels of short‐chain fatty acids in the colon, highlighting their enhanced ...
Lingyan Ma, Shiyu Tao, Tongxing Song, Wentao Lyu, Ying Li, Wen Wang, Qicheng Shen, Yan Ni, Jiang Zhu, Jiangchao Zhao, Hua Yang, Yingping Xiao   +11 more
wiley   +1 more source

An extracellular glucose sensor for substrate‐dependent secretion and display of cellulose‐degrading enzymes

Biotechnology and Bioengineering, Volume 121, Issue 1, Page 403-408, January 2024.
An extracellular glucose sensor was developed for the yeast Saccharomyces cerevisiae to enable substrate‐dependent secretion and surface display of cellulases. Promoter and strain engineering increased the performance of the system, and cellobiose‐dependent signal amplification resulted in a multi‐fold increase in extracellular enzyme activity ...
Pamela B. Besada‐Lombana, Wilfred Chen, Nancy A. Da Silva   +2 more
wiley   +1 more source

Rumen cellulosomics: divergent fiber-degrading strategies revealed by comparative genome-wide analysis of six ruminococcal strains.

PLoS ONE, 2014
BackgroundA complex community of microorganisms is responsible for efficient plant cell wall digestion by many herbivores, notably the ruminants. Understanding the different fibrolytic mechanisms utilized by these bacteria has been of great interest in ...
Bareket Dassa, Ilya Borovok, Vered Ruimy-Israeli, Raphael Lamed, Harry J Flint, Sylvia H Duncan, Bernard Henrissat, Pedro Coutinho, Mark Morrison, Pascale Mosoni, Carl J Yeoman, Bryan A White, Edward A Bayer   +12 more
doaj   +1 more source

Functional characterization of a dockerin-containing expansin-like protein from the anaerobic fungus Neocallimastix californiae

Biotechnology for Biofuels and Bioproducts
Background Anaerobic microbes produce multienzyme complexes known as cellulosomes to enhance the degradation of cellulosic substrates. These complexes localize diverse enzymes onto a protein scaffold, where proteins are anchored by dockerin domains ...
Taru Koitto, Anna Pohto, Elizaveta Sidorova, Thu V. Vuong, Merja Penttilä, Emma R. Master   +5 more
doaj   +1 more source

A high throughput approach for the generation of orthogonally interacting protein pairs

Scientific Reports, 2018
In contrast to the nearly error-free self-assembly of protein architectures in nature, artificial assembly of protein complexes with pre-defined structure and function in vitro is still challenging.
Justin Lawrie, Xi Song, Wei Niu, Jiantao Guo   +3 more
doaj   +1 more source

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction

Scientific Reports, 2018
Cellulosomes are highly sophisticated molecular nanomachines that participate in the deconstruction of complex polysaccharides, notably cellulose and hemicellulose.
Pedro Bule, Virgínia M. R. Pires, Victor D. Alves, Ana Luísa Carvalho, José A. M. Prates, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Ilit Noach, Edward A. Bayer, Shabir Najmudin, Carlos M. G. A. Fontes   +11 more
doaj   +1 more source

Diversity of microbial carbohydrate-active enzymes in Danish anaerobic digesters fed with wastewater treatment sludge

Biotechnology for Biofuels, 2017
Background Improved carbohydrate-active enzymes (CAZymes) are needed to fulfill the goal of producing food, feed, fuel, chemicals, and materials from biomass.
Casper Wilkens, Peter Kamp Busk, Bo Pilgaard, Wen-Jing Zhang, Kåre L. Nielsen, Per Halkjær Nielsen, Lene Lange   +6 more
doaj   +1 more source