Results 71 to 80 of about 1,595 (147)

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction

Scientific Reports, 2018
Cellulosomes are highly sophisticated molecular nanomachines that participate in the deconstruction of complex polysaccharides, notably cellulose and hemicellulose.
Pedro Bule, Virgínia M. R. Pires, Victor D. Alves, Ana Luísa Carvalho, José A. M. Prates, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Ilit Noach, Edward A. Bayer, Shabir Najmudin, Carlos M. G. A. Fontes   +11 more
doaj   +1 more source

Diversity of microbial carbohydrate-active enzymes in Danish anaerobic digesters fed with wastewater treatment sludge

Biotechnology for Biofuels, 2017
Background Improved carbohydrate-active enzymes (CAZymes) are needed to fulfill the goal of producing food, feed, fuel, chemicals, and materials from biomass.
Casper Wilkens, Peter Kamp Busk, Bo Pilgaard, Wen-Jing Zhang, Kåre L. Nielsen, Per Halkjær Nielsen, Lene Lange   +6 more
doaj   +1 more source

Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex

, 2003
The utilization of organized supramolecular assemblies to exploit the synergistic interactions afforded by close proximity, both for enzymatic synthesis and for the degradation of recalcitrant substrates, is an emerging theme in cellular biology ...
Davies, Gideon J., Carvalho, Ana L., Nagy, Tibor, Gilbert, Harry J., Ferreira, Luís M. A., Prates, José A. M., Fontes, Carlos M. G. A., Dias, Fernanda M. V., Romão, Maria J.   +8 more
core   +1 more source

Transcriptomic characterization of Caecomyces churrovis: a novel, non-rhizoid-forming lignocellulolytic anaerobic fungus

Biotechnology for Biofuels, 2017
Anaerobic gut fungi are the primary colonizers of plant material in the rumen microbiome, but are poorly studied due to a lack of characterized isolates.
John K. Henske, Sean P. Gilmore, Doriv Knop, Francis J. Cunningham, Jessica A. Sexton, Chuck R. Smallwood, Vaithiyalingam Shutthanandan, James E. Evans, Michael K. Theodorou, Michelle A. O’Malley   +9 more
doaj   +1 more source

Structure and function relationships in novel cohesin-dockerin complexes

, 2015
Tese de Doutoramento em Ciências Veterinárias - Especialidade de Ciências Biológicas e BiomédicasCohesin-dockerin interactions orchestrate the assembly of carbohydrate degrading multi-enzyme complexes produced by anaerobic bacteria termed cellulosomes ...
Cameron, Kate
core   +3 more sources

Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome

Microbial Cell Factories, 2011
Background The recalcitrant nature of cellulosic materials and the high cost of enzymes required for efficient hydrolysis are the major impeding steps to their practical usage for ethanol production.
Madan Bhawna, Tsai Shen-Long, Goyal Garima, DaSilva Nancy A, Chen Wilfred   +4 more
doaj   +1 more source

Combined Crystal Structure of a Type I Cohesin: Mutation and Affinity Binding Studies Reveal Structural Determinants of Cohesin-Dockerin Specificities

Background: Cellulosomal cohesin-dockerin types are reversed in Bacteroides cellulosolvens. Results: Combined crystallographic and computational approaches of a lone cohesin yielded a structural model of the cohesin-dockerin complex that was verified ...
Cameron K, Zhivin O, Fontes CMGA, Gilbert HJ, Najmudin S, Weinstein JY, Alves VD, Ferreira LMA, Bayer EA, Fleishman SJ, Bule P   +10 more
core   +4 more sources

Engineering an artificial catch bond using mechanical anisotropy

Nature Communications
Catch bonds are a rare class of protein-protein interactions where the bond lifetime increases under an external pulling force. Here, we report how modification of anchor geometry generates catch bonding behavior for the mechanostable Dockerin G:Cohesin ...
Zhaowei Liu, Haipei Liu, Andrés M. Vera, Byeongseon Yang, Philip Tinnefeld, Michael A. Nash   +5 more
doaj   +1 more source

Deconstruction of Lignocellulose into Soluble Sugars by Native and Designer Cellulosomes

mBio, 2012
Lignocellulosic biomass, the most abundant polymer on Earth, is typically composed of three major constituents: cellulose, hemicellulose, and lignin. The crystallinity of cellulose, hydrophobicity of lignin, and encapsulation of cellulose by the lignin ...
Sarah Moraïs, Ely Morag, Yoav Barak, Dan Goldman, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, Edward A. Bayer   +8 more
doaj   +1 more source

Role of pectinolytic enzymes identified in Clostridium thermocellum cellulosome.

PLoS ONE, 2015
The cloning, expression and characterization of three cellulosomal pectinolytic enzymes viz., two variants of PL1 (PL1A and PL1B) and PL9 from Clostridium thermocellum was carried out.
Soumyadeep Chakraborty, Vania O Fernandes, Fernando M V Dias, Jose A M Prates, Luis M A Ferreira, Carlos M G A Fontes, Arun Goyal, Maria S J Centeno   +7 more
doaj   +1 more source