Results 81 to 90 of about 139 (126)

Transcriptomic characterization of Caecomyces churrovis: a novel, non-rhizoid-forming lignocellulolytic anaerobic fungus

Biotechnology for Biofuels, 2017
Anaerobic gut fungi are the primary colonizers of plant material in the rumen microbiome, but are poorly studied due to a lack of characterized isolates.
John K. Henske, Sean P. Gilmore, Doriv Knop, Francis J. Cunningham, Jessica A. Sexton, Chuck R. Smallwood, Vaithiyalingam Shutthanandan, James E. Evans, Michael K. Theodorou, Michelle A. O’Malley   +9 more
doaj   +1 more source

Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome

Microbial Cell Factories, 2011
Background The recalcitrant nature of cellulosic materials and the high cost of enzymes required for efficient hydrolysis are the major impeding steps to their practical usage for ethanol production.
Madan Bhawna, Tsai Shen-Long, Goyal Garima, DaSilva Nancy A, Chen Wilfred   +4 more
doaj   +1 more source

Engineering an artificial catch bond using mechanical anisotropy

Nature Communications
Catch bonds are a rare class of protein-protein interactions where the bond lifetime increases under an external pulling force. Here, we report how modification of anchor geometry generates catch bonding behavior for the mechanostable Dockerin G:Cohesin ...
Zhaowei Liu, Haipei Liu, Andrés M. Vera, Byeongseon Yang, Philip Tinnefeld, Michael A. Nash   +5 more
doaj   +1 more source

Deconstruction of Lignocellulose into Soluble Sugars by Native and Designer Cellulosomes

mBio, 2012
Lignocellulosic biomass, the most abundant polymer on Earth, is typically composed of three major constituents: cellulose, hemicellulose, and lignin. The crystallinity of cellulose, hydrophobicity of lignin, and encapsulation of cellulose by the lignin ...
Sarah Moraïs, Ely Morag, Yoav Barak, Dan Goldman, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, Edward A. Bayer   +8 more
doaj   +1 more source

Role of pectinolytic enzymes identified in Clostridium thermocellum cellulosome.

PLoS ONE, 2015
The cloning, expression and characterization of three cellulosomal pectinolytic enzymes viz., two variants of PL1 (PL1A and PL1B) and PL9 from Clostridium thermocellum was carried out.
Soumyadeep Chakraborty, Vania O Fernandes, Fernando M V Dias, Jose A M Prates, Luis M A Ferreira, Carlos M G A Fontes, Arun Goyal, Maria S J Centeno   +7 more
doaj   +1 more source

Clostridium clariflavum: Key Cellulosome Players Are Revealed by Proteomic Analysis

mBio, 2015
Clostridium clariflavum is an anaerobic, cellulosome-forming thermophile, containing in its genome genes for a large number of cellulosomal enzyme and a complex scaffoldin system.
Lior Artzi, Ely Morag, Yoav Barak, Raphael Lamed, Edward A. Bayer   +4 more
doaj   +1 more source

Impact of pretreated Switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis.

PLoS ONE, 2009
BackgroundEconomic feasibility and sustainability of lignocellulosic ethanol production requires the development of robust microorganisms that can efficiently degrade and convert plant biomass to ethanol.
Babu Raman, Chongle Pan, Gregory B Hurst, Miguel Rodriguez, Catherine K McKeown, Patricia K Lankford, Nagiza F Samatova, Jonathan R Mielenz   +7 more
doaj   +1 more source

Limousia bacteria encode mucinolysome for mucin utilization in animal gut microbiomes

Gut Microbes
Mucins create a physical barrier that protects human and animal tissues from microbial pathogens. Here, we provide evidence that mucin degradation can be mediated by unique mucinolysomes, defined as extracellular cellulosome-like multi-enzyme complexes ...
Jerry Elorm Akresi, Thi Van Thanh Do, Zhisong Cui, N. R. Siva Shanmugam, Sarah Moraïs, Itzhak Mizrahi, Edward A. Bayer, Jennifer M. Auchtung, Yanbin Yin   +8 more
doaj   +1 more source

HLA-DM co-expression enhances MHC class II function in the magnetosome display system

Microbiology Spectrum
Efficient identification of antigenic peptides that bind to major histocompatibility complex class II (MHC II) molecules is crucial for vaccine and therapeutic development.
Ryoto Tomoe, Toru Honda, Tsuyoshi Tanaka, Tomoko Yoshino   +3 more
doaj   +1 more source

Detecting motifs for computational classification of dockerin and cohesin sequences [PDF]

, 2013
Cellulose is the most abundant biopolymer in nature. It has several usage areas in industry.The initial hydrolysis of cellulose is the rate determining step in cellulose degradation. Cellulosomesare the complex structures composed of non-catalytic units and enzymes thattake part in cellulose degradation.
openaire   +1 more source