Results 101 to 110 of about 1,461 (191)

A high throughput approach for the generation of orthogonally interacting protein pairs

Scientific Reports, 2018
In contrast to the nearly error-free self-assembly of protein architectures in nature, artificial assembly of protein complexes with pre-defined structure and function in vitro is still challenging.
Justin Lawrie, Xi Song, Wei Niu, Jiantao Guo   +3 more
doaj   +1 more source

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction

Scientific Reports, 2018
Cellulosomes are highly sophisticated molecular nanomachines that participate in the deconstruction of complex polysaccharides, notably cellulose and hemicellulose.
Pedro Bule, Virgínia M. R. Pires, Victor D. Alves, Ana Luísa Carvalho, José A. M. Prates, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Ilit Noach, Edward A. Bayer, Shabir Najmudin, Carlos M. G. A. Fontes   +11 more
doaj   +1 more source

Coarse-Grained MD Simulations and Protein−Protein Interactions: The Cohesin−Dockerin System

, 2016
Coarse-grained molecular dynamics (CG-MD) may be applied as part of a multiscale modeling approach to protein−protein interactions. The cohesin−dockerin interaction provides a valuable test system for evaluation of the use of CG-MD, as structural (X-ray)
Benjamin A. Hall (199856), Mark S. P. Sansom (147715)   +1 more
core   +1 more source

Dockerin soveltuvuus teollisen internetin yhdyskäytäviin

, 2018
In the past few years, Linux container implementation Docker, which also provides the means to distribute, control, manage and orchestrate the containers, has been increasingly adopted in software development and the cloud. Docker provides an easy way to
Lumio, Niklas
core  

Diversity of microbial carbohydrate-active enzymes in Danish anaerobic digesters fed with wastewater treatment sludge

Biotechnology for Biofuels, 2017
Background Improved carbohydrate-active enzymes (CAZymes) are needed to fulfill the goal of producing food, feed, fuel, chemicals, and materials from biomass.
Casper Wilkens, Peter Kamp Busk, Bo Pilgaard, Wen-Jing Zhang, Kåre L. Nielsen, Per Halkjær Nielsen, Lene Lange   +6 more
doaj   +1 more source

Transcriptomic characterization of Caecomyces churrovis: a novel, non-rhizoid-forming lignocellulolytic anaerobic fungus

Biotechnology for Biofuels, 2017
Anaerobic gut fungi are the primary colonizers of plant material in the rumen microbiome, but are poorly studied due to a lack of characterized isolates.
John K. Henske, Sean P. Gilmore, Doriv Knop, Francis J. Cunningham, Jessica A. Sexton, Chuck R. Smallwood, Vaithiyalingam Shutthanandan, James E. Evans, Michael K. Theodorou, Michelle A. O’Malley   +9 more
doaj   +1 more source

Comparison of the number of putative dockerin containing GH sequences in selected Clostridial species.

, 2014
C.phytofermentans ISDg and C.stercorarium DSM 8532 have no detectable dockerin domains.
John Schellenberg (610765), Bernard Henrissat (31550), Richard Sparling (394480), Riffat I. Munir (610764), David B. Levin (330149), Tobin J. Verbeke (394474)   +5 more
core   +1 more source

Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome

Microbial Cell Factories, 2011
Background The recalcitrant nature of cellulosic materials and the high cost of enzymes required for efficient hydrolysis are the major impeding steps to their practical usage for ethanol production.
Madan Bhawna, Tsai Shen-Long, Goyal Garima, DaSilva Nancy A, Chen Wilfred   +4 more
doaj   +1 more source

Engineering an artificial catch bond using mechanical anisotropy

Nature Communications
Catch bonds are a rare class of protein-protein interactions where the bond lifetime increases under an external pulling force. Here, we report how modification of anchor geometry generates catch bonding behavior for the mechanostable Dockerin G:Cohesin ...
Zhaowei Liu, Haipei Liu, Andrés M. Vera, Byeongseon Yang, Philip Tinnefeld, Michael A. Nash   +5 more
doaj   +1 more source

Deconstruction of Lignocellulose into Soluble Sugars by Native and Designer Cellulosomes

mBio, 2012
Lignocellulosic biomass, the most abundant polymer on Earth, is typically composed of three major constituents: cellulose, hemicellulose, and lignin. The crystallinity of cellulose, hydrophobicity of lignin, and encapsulation of cellulose by the lignin ...
Sarah Moraïs, Ely Morag, Yoav Barak, Dan Goldman, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, Edward A. Bayer   +8 more
doaj   +1 more source