Results 111 to 120 of about 1,461 (191)

Site-Specific and High-Loading Immobilization of Proteins by Using Cohesin–Dockerin and CBM–Cellulose Interactions

, 2016
Immobilization of enzymes enhances their properties for application in industrial processes as reusable and robust biocatalysts. Here, we developed a new immobilization method by mimicking the natural cellulosome system.
Zai-Yu Wang (2864462), Li-Hai Fan (2864453), Zi-Jian Zhang (2864459), Mei Li (217363), Yi Yue (403700), Tian-Wei Tan (2864456)   +5 more
core   +1 more source

Role of pectinolytic enzymes identified in Clostridium thermocellum cellulosome.

PLoS ONE, 2015
The cloning, expression and characterization of three cellulosomal pectinolytic enzymes viz., two variants of PL1 (PL1A and PL1B) and PL9 from Clostridium thermocellum was carried out.
Soumyadeep Chakraborty, Vania O Fernandes, Fernando M V Dias, Jose A M Prates, Luis M A Ferreira, Carlos M G A Fontes, Arun Goyal, Maria S J Centeno   +7 more
doaj   +1 more source

Clostridium clariflavum: Key Cellulosome Players Are Revealed by Proteomic Analysis

mBio, 2015
Clostridium clariflavum is an anaerobic, cellulosome-forming thermophile, containing in its genome genes for a large number of cellulosomal enzyme and a complex scaffoldin system.
Lior Artzi, Ely Morag, Yoav Barak, Raphael Lamed, Edward A. Bayer   +4 more
doaj   +1 more source

Duplicated Dockerin Subdomains of Clostridium thermocellum Endoglucanase CelD Bind to a Cohesin Domain of the Scaffolding Protein CipA with Distinct Thermodynamic Parameters and a Negative Cooperativity

, 2002
International audienceMutagenized dockerin domains of endoglucanase CelD (type I) and of the cellulosome-integrating protein CipA (type II) were constructed by swapping residues 10 and 11 of the first or the second duplicated segment between the two ...
Guglielmi, Gérard, Miras, Isabelle, Matuschek, Markus, Schaeffer, Francis, Béguin, Pierre, Alzari, Pedro   +5 more
core   +1 more source

Moderni sovellushallinta Dockerin avulla

Tässä opinnäytetyössä käsitellään modernia sovellushallinta työkalua Dockeria. Dockerin avulla voidaan pitää sovellukset erillään, paketoida niitä ja parantaa kehitysprosessien tehokkuutta ja joustavuutta.
Le, Trinh
core  

Escherichia coli Expression, Purification, Crystallization, and Structure Determination of Bacterial Cohesin–Dockerin Complexes

, 2012
Cellulosomes are highly efficient nanomachines that play a fundamental role during the anaerobic deconstruction of complex plant cell wall carbohydrates. The assembly of these complex nanomachines results from the very tight binding of repetitive cohesin
Carvalho AL, Romao MJ, Fontes CMGA, Gilbert HJ, Najmudin S, Prates JAM, Alves VD, Ferreira LMA, Viegas A, Bras JLA   +9 more
core  

Impact of pretreated Switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis.

PLoS ONE, 2009
BackgroundEconomic feasibility and sustainability of lignocellulosic ethanol production requires the development of robust microorganisms that can efficiently degrade and convert plant biomass to ethanol.
Babu Raman, Chongle Pan, Gregory B Hurst, Miguel Rodriguez, Catherine K McKeown, Patricia K Lankford, Nagiza F Samatova, Jonathan R Mielenz   +7 more
doaj   +1 more source

Duplicated Dockerin Subdomains of Clostridium thermocellum Endoglucanase CelD Bind to a Cohesin Domain of the Scaffolding Protein CipA with Distinct Thermodynamic Parameters and a Negative Cooperativity^†

, 2016
Mutagenized dockerin domains of endoglucanase CelD (type I) and of the cellulosome-integrating protein CipA (type II) were constructed by swapping residues 10 and 11 of the first or the second duplicated segment between the two polypeptides.
Pierre Béguin (2946444), Gérard Guglielmi (2946450), Pedro M. Alzari (106750), Francis Schaeffer (106729), Isabelle Miras (2946441), Markus Matuschek (2946447)   +5 more
core   +1 more source

Divergent cellulosome architecture in rumen bacteria : structure and function studies in cohesin-dockerin complexes of Ruminococcus flavefaciens

, 2017
Tese de Doutoramento em Ciências Veterinárias, na Especialidade de Produção AnimalProtein-protein interactions play a vital role in many cellular processes as exemplified by the assembly of the cellulosome, a bacterial multi-enzyme complex that ...
Gomes, Pedro Miguel Bule
core   +2 more sources

The Clostridium cellulolyticum Dockerin Displays a Dual Binding Mode for Its Cohesin Partner

The plant cell wall degrading apparatus of anaerobic bacteria includes a large multienzyme complex termed the "cellulosome." The complex assembles through the interaction of enzyme-derived dockerin modules with the multiple cohesin modules of the ...
Money VA, Gilbert HJ, Fierobe HP, Martinez-Fleites C, Davies GJ, Bayer EA, Proctor MR, Pinheiro BA, Prates JA, Fontesm CM   +9 more
core