Results 91 to 100 of about 1,461 (191)

Molecular mechanics studies of factors affecting overall rate in cascade reactions: Multi‐enzyme colocalization and environment

Protein Science, Volume 33, Issue 10, October 2024.
Abstract Millions of years of evolution have optimized many biosynthetic pathways by use of multi‐step catalysis. In addition, multi‐step metabolic pathways are commonly found in and on membrane‐bound organelles in eukaryotic biochemistry. The fundamental mechanisms that facilitate these reaction processes provide strategies to bioengineer metabolic ...
Shivansh Kaushik, Ta I Hung, Chia‐en A. Chang   +2 more
wiley   +1 more source

Dockerin-like sequences in cellulases and xylanases from the rumen cellulolytic bacterium Ruminococcus flavefaciens [PDF]

FEMS Microbiology Letters, 2006
Recent analysis of the endA cellulase gene from Ruminococcus flavefaciens 17 has revealed that it encodes a product of 759 amino acids that provides the first example of a multidomain cellulase from a Ruminococcus sp. Following the family 5 catalytic domain in the predicted EndA enzyme is a 282 amino acid domain of unknown function for which no close ...
J, Kirby, J C, Martin, A S, Daniel, H J, Flint   +3 more
openaire   +2 more sources

Structural and molecular insights into a bifunctional glycoside hydrolase 30 xylanase specific to glucuronoxylan

Biotechnology and Bioengineering, Volume 121, Issue 7, Page 2067-2078, July 2024.
Abstract Glycoside hydrolase (GH) 30 family xylanases are enzymes of biotechnological interest due to their capacity to degrade recalcitrant hemicelluloses, such as glucuronoxylan (GX). This study focuses on a subfamily 7 GH30, TtXyn30A from Thermothelomyces thermophilus, which acts on GX in an “endo” and “exo” mode, releasing methyl‐glucuronic acid ...
Christina Pentari, Christos Kosinas, Efstratios Nikolaivits, Maria Dimarogona, Evangelos Topakas   +4 more
wiley   +1 more source

Clostridium butyricum and carbohydrate active enzymes contribute to the reduced fat deposition in pigs

iMeta, Volume 3, Issue 1, February 2024.
In the present study, we found significant differences in microbial composition and potential functional capacity among different gut locations in Jinhua pigs with distinct fatness phenotypes. Second, we identified that Jinhua pigs with lower fatness exhibited higher levels of short‐chain fatty acids in the colon, highlighting their enhanced ...
Lingyan Ma, Shiyu Tao, Tongxing Song, Wentao Lyu, Ying Li, Wen Wang, Qicheng Shen, Yan Ni, Jiang Zhu, Jiangchao Zhao, Hua Yang, Yingping Xiao   +11 more
wiley   +1 more source

Unraveling enzyme discrimination during cellulosome assembly independent of cohesin–dockerin affinity [PDF]

The FEBS Journal, 2013
Bacterial cellulosomes are generally believed to assemble at random, like those produced by Clostridium cellulolyticum. They are composed of one scaffolding protein bearing eight homologous type I cohesins that bind to any of the type I dockerins borne by the 62 cellulosomal subunits, thus generating highly heterogeneous complexes. In the present study,
Romain, Borne, Edward A, Bayer, Sandrine, Pagès, Stéphanie, Perret, Henri-Pierre, Fierobe   +4 more
openaire   +2 more sources

Comparative analysis of carbohydrate active enzymes in Clostridium termitidis CT1112 reveals complex carbohydrate degradation ability.

PLoS ONE, 2014
Clostridium termitidis strain CT1112 is an anaerobic, gram positive, mesophilic, cellulolytic bacillus isolated from the gut of the wood-feeding termite, Nasutitermes lujae.
Riffat I Munir, John Schellenberg, Bernard Henrissat, Tobin J Verbeke, Richard Sparling, David B Levin   +5 more
doaj   +1 more source

Dockerin rooli kestävässä ohjelmistokehityksessä [PDF]

Tutkimuksen tavoitteena oli tutkia Dockerin vaikutusta ja arvioida sen merkitystä kestävässä ohjelmistokehityksessä. Opinnäytetyön tarkoituksena oli selvittää, kuinka Dockerin käyttö vaikuttaa resurssien hallintaan ja sovelluksen suorituskykyyn ...
Syrjälä, Tanika
core  

An extracellular glucose sensor for substrate‐dependent secretion and display of cellulose‐degrading enzymes

Biotechnology and Bioengineering, Volume 121, Issue 1, Page 403-408, January 2024.
An extracellular glucose sensor was developed for the yeast Saccharomyces cerevisiae to enable substrate‐dependent secretion and surface display of cellulases. Promoter and strain engineering increased the performance of the system, and cellobiose‐dependent signal amplification resulted in a multi‐fold increase in extracellular enzyme activity ...
Pamela B. Besada‐Lombana, Wilfred Chen, Nancy A. Da Silva   +2 more
wiley   +1 more source

Rumen cellulosomics: divergent fiber-degrading strategies revealed by comparative genome-wide analysis of six ruminococcal strains.

PLoS ONE, 2014
BackgroundA complex community of microorganisms is responsible for efficient plant cell wall digestion by many herbivores, notably the ruminants. Understanding the different fibrolytic mechanisms utilized by these bacteria has been of great interest in ...
Bareket Dassa, Ilya Borovok, Vered Ruimy-Israeli, Raphael Lamed, Harry J Flint, Sylvia H Duncan, Bernard Henrissat, Pedro Coutinho, Mark Morrison, Pascale Mosoni, Carl J Yeoman, Bryan A White, Edward A Bayer   +12 more
doaj   +1 more source

Design and production in Aspergillus niger of a chimeric protein associating a fungal feruloyl esterase and a clostridial dockerin domain

, 2004
A chimeric enzyme associating feruloyl esterase A (FAEA) from Aspergilhis niger and dockerin from Clostridium thermocellum was produced in A. niger. A completely truncated form was produced when the dockerin domain was located downstream of the FAEA ...
Asther, M., J.-P. Belaich, Navarro, D., Pages, Sandrine, Punt, Peter, Pagès, S., Levasseur, Anthony, Belaich, Jean-Pierre, Record, E., Navarro, David, Fierobe, H.-P., H.-P. Fierobe, Belaïch, J.-P., Asther, Marcel, M., Levasseur, A., Fierobe, Henri-Pierre, Asther, Marcel,, Record, Eric, S. Pages, E. Record, A. Levasseur, D. Navarro, P. Punt, M. Asther, Punt, P.   +24 more
core   +1 more source