Results 61 to 70 of about 1,461 (191)
Novel Organization and Divergent Dockerin Specificities in the Cellulosome System of Ruminococcus flavefaciens [PDF]
Journal of Bacteriology, 2003 ABSTRACT The DNA sequence coding for putative cellulosomal scaffolding protein ScaA from the rumen cellulolytic anaerobe Ruminococcus flavefaciens 17 was completed. The mature protein exhibits a calculated molecular mass of 90,198 Da and comprises three cohesin domains, a C-terminal dockerin, and a unique ...
Rincon M.T.1 +10 more
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mBio, 2015 Ruminococcus bromii is a dominant member of the human gut microbiota that plays a key role in releasing energy from dietary starches that escape digestion by host enzymes via its exceptional activity against particulate “resistant” starches.
Xiaolei Ze +11 more
doaj +1 more source
Microbial Cell Factories, 2013 Background Cellulosic biomass is considered as a promising alternative to fossil fuels, but its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this material.
Kim Sujin +3 more
doaj +1 more source
Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature
mBio, 2016 Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes. The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
Johanna Stern +3 more
doaj +1 more source
The Clostridium cellulolyticum Dockerin Displays a Dual Binding Mode for Its Cohesin Partner [PDF]
Journal of Biological Chemistry, 2008 The plant cell wall degrading apparatus of anaerobic bacteria includes a large multienzyme complex termed the "cellulosome." The complex assembles through the interaction of enzyme-derived dockerin modules with the multiple cohesin modules of the noncatalytic scaffolding protein.
Pinheiro BA +9 more
openaire +3 more sources
Fermentation, 2022 Cellulosome is a highly efficient multi-enzyme self-assembly system and is found on the extracellular surface or in the free environment of microorganisms. However, with a lack of Ca2+ in vivo, cellulosome assembly is challenging.
Nan Li +9 more
doaj +1 more source
Mapping the deformability of natural and designed cellulosomes in solution
Biotechnology for Biofuels and Bioproducts, 2022 Background Natural cellulosome multi-enzyme complexes, their components, and engineered ‘designer cellulosomes’ (DCs) promise an efficient means of breaking down cellulosic substrates into valuable biofuel products.
Jonathan Dorival +11 more
doaj +1 more source
Characterization and subcellular localization of the Clostridium thermocellum scaffoldin dockerin binding protein SdbA [PDF]
Journal of Bacteriology, 1997 This article reports the characterization of the Clostridium thermocellum SdbA protein thought to anchor the cellulosome to the bacterial cell surface. The NH2-terminal region of SdbA consists of a cohesin domain which specifically binds the dockerin domain of the cellulosomal scaffolding protein CipA. The COOH-terminal region consists of a triplicated
E, Leibovitz +3 more
openaire +2 more sources
CRISPR-PIN: Modifying gene position in the nucleus via dCas9-mediated tethering
Synthetic and Systems Biotechnology, 2019 Spatial organization of DNA within the nucleus is important for controlling DNA replication and repair, genetic recombination, and gene expression. Here, we present CRISPR-PIN, a CRISPR/dCas9-based tool that allows control of gene Position in the Nucleus
Jyun-Liang Lin +3 more
doaj +1 more source
Frontiers in Microbiology, 2021 The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential.
Timothy J. Williams +3 more
doaj +1 more source