Results 11 to 20 of about 139 (126)

Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome‐Based Synthetic Protein Scaffold

Microbial Biotechnology
In systems biocatalysis, combining pathway enzymes in vitro allows for the conversion of basic substrates into more complex, valuable chemicals. However, in vitro enzyme cascades are not yet economically viable for large‐scale bio‐based chemical ...
Marte Elias, Kenan Meert, Julie Vanderstraeten, Babette Lamote, Yves Briers   +4 more
doaj   +2 more sources

Designing chimeric enzymes inspired by fungal cellulosomes

Synthetic and Systems Biotechnology, 2020
Cellulosomes are synthesized by anaerobic bacteria and fungi to degrade lignocellulose via synergistic action of multiple enzymes fused to a protein scaffold.
Sean P. Gilmore, Stephen P. Lillington, Charles H. Haitjema, Randall de Groot, Michelle A. O'Malley   +4 more
doaj   +1 more source

Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin–dockerin interactions [PDF]

PeerJ, 2014
Cellulosic waste represents a significant and underutilized carbon source for the biofuel industry. Owing to the recalcitrance of crystalline cellulose to enzymatic degradation, it is necessary to design economical methods of liberating the fermentable ...
Yuval Hamberg, Vered Ruimy-Israeli, Bareket Dassa, Yoav Barak, Raphael Lamed, Kate Cameron, Carlos M.G.A. Fontes, Edward A. Bayer, Daniel B. Fried   +8 more
doaj   +2 more sources

Effects of dockerin domains onNeocallimastix frontalisxylanases [PDF]

FEMS Microbiology Letters, 2005
Two xylanase genes were cloned from the anaerobic fungus Neocallimastix frontalis. Xyn11A had a modular structure of two catalytic domains and two dockerin domains, while Xyn11B had one catalytic domain and two dockerin domains. The characteristics of the xylanases with and without dockerin domains were investigated.
Ya-Hui, Huang, Ching-Tsan, Huang, Ruey-Shyang, Hseu   +2 more
openaire   +2 more sources

Cellulosome assembly revealed by the crystal structure of the cohesin–dockerin complex [PDF]

Proceedings of the National Academy of Sciences, 2003
The utilization of organized supramolecular assemblies to exploit the synergistic interactions afforded by close proximity, both for enzymatic synthesis and for the degradation of recalcitrant substrates, is an emerging theme in cellular biology.
Carvalho AL, Dias FMV, Prates JAM, Nagy T, Gilbert HJ, Davies GJ, Ferreira LMA, Romao MJ, Fontes CMGA   +8 more
openaire   +3 more sources

Cellulosomics, a gene-centric approach to investigating the intraspecific diversity and adaptation of Ruminococcus flavefaciens within the rumen. [PDF]

PLoS ONE, 2011
BackgroundThe bovine rumen maintains a diverse microbial community that serves to break down indigestible plant substrates. However, those bacteria specifically adapted to degrade cellulose, the major structural component of plant biomass, represent a ...
Jennifer M Brulc, Carl J Yeoman, Melissa K Wilson, Margret E Berg Miller, Patricio Jeraldo, Sadanari Jindou, Nigel Goldenfeld, Harry J Flint, Raphael Lamed, Ilya Borovok, Maša Vodovnik, Karen E Nelson, Edward A Bayer, Bryan A White   +13 more
doaj   +1 more source

Noncellulosomal cohesin- and dockerin-like modules in the three domains of life [PDF]

FEMS Microbiology Letters, 2009
The high-affinity cohesin-dockerin interaction was originally discovered as modular components, which mediate the assembly of the various subunits of the multienzyme cellulosome complex that characterizes some cellulolytic bacteria. Until recently, the presence of cohesins and dockerins within a bacterial proteome was considered a definitive signature ...
Ayelet, Peer, Steven P, Smith, Edward A, Bayer, Raphael, Lamed, Ilya, Borovok   +4 more
openaire   +2 more sources

Co‑cultivation of the anaerobic fungus Caecomyces churrovis with Methanobacterium bryantii enhances transcription of carbohydrate binding modules, dockerins, and pyruvate formate lyases on specific substrates

Biotechnology for Biofuels, 2021
Anaerobic fungi and methanogenic archaea are two classes of microorganisms found in the rumen microbiome that metabolically interact during lignocellulose breakdown.
Jennifer L. Brown, Candice L. Swift, Stephen J. Mondo, Susanna Seppala, Asaf Salamov, Vasanth Singan, Bernard Henrissat, Elodie Drula, John K. Henske, Samantha Lee, Kurt LaButti, Guifen He, Mi Yan, Kerrie Barry, Igor V. Grigoriev, Michelle A. O’Malley   +15 more
doaj   +1 more source

Cohesin-dockerin interactions within and between Clostridium josui and Clostridium thermocellum: binding selectivity between cognate dockerin and cohesin domains and species specificity. [PDF]

The Journal of biological chemistry, 2004
The cellulosome components are assembled into the cellulosome complex by the interaction between one of the repeated cohesin domains of a scaffolding protein and the dockerin domain of an enzyme component. We prepared five recombinant cohesin polypeptides of the Clostridium thermocellum scaffolding protein CipA, two dockerin polypeptides of C ...
Sadanari, Jindou, Akane, Soda, Shuichi, Karita, Tsutomu, Kajino, Pierre, Béguin, J H David, Wu, Minoru, Inagaki, Tetsuya, Kimura, Kazuo, Sakka, Kunio, Ohmiya   +9 more
openaire   +2 more sources

Protein Engineering and High‐Throughput Screening by Yeast Surface Display: Survey of Current Methods

Small Science, Volume 3, Issue 12, December 2023., 2023
Presenting a focused review on novel methods for protein engineering, this article delves into cutting‐edge advancements in the field of yeast surface display. Central themes include titratable avidity, on‐yeast serology, unconventional antigens including cell lysates and membrane proteins, expanded chemistry, noncanonical amino acids, and continuous ...
Joanan Lopez-Morales, Rosario Vanella, Elizabeth A. Appelt, Sarah Whillock, Alexandra M. Paulk, Eric V. Shusta, Benjamin J. Hackel, Chang C. Liu, Michael A. Nash   +8 more
wiley   +1 more source