A Scaffoldomics Platform for Modular In Vivo Enzyme Colocalisation and Its Application to Naringenin Biosynthesis. [PDF]
Microb BiotechnolA hierarchical assembly system was established in which the coding sequences for four docking enzymes and a scaffold are co‐assembled onto a single plasmid, thereby establishing a ‘scaffoldomics’ platform for the optimisation of multi‐enzyme biosynthetic pathways.
Elias M +5 more
europepmc +2 more sources
Evolution of N-terminal mechanical lability as a determinant for Type III secretion. [PDF]
Protein SciAbstract Many Gram‐negative pathogens critically depend on the Type III secretion system (T3SS) to inject effector proteins into host cells for colonization. Because the channel of the T3SS is narrow (~2 nm), effectors must be unfolded for secretion.
DaPron KE +7 more
europepmc +2 more sources
Integrative SAXS and AFM analysis of engineered carbohydrate-active enzyme assemblies with tunable spatial organization. [PDF]
Protein SciAbstract Cellulosomes are multi‐enzyme assemblies whose catalytic efficiency depends on the spatial organization of their components. However, their pronounced conformational flexibility has precluded quantitative characterization of inter‐enzyme distances and overall topology.
Pardo Larrabeiti I +11 more
europepmc +2 more sources
Designing chimeric enzymes inspired by fungal cellulosomes
Synthetic and Systems Biotechnology, 2020 Cellulosomes are synthesized by anaerobic bacteria and fungi to degrade lignocellulose via synergistic action of multiple enzymes fused to a protein scaffold.
Sean P. Gilmore +4 more
doaj +1 more source
Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin–dockerin interactions [PDF]
PeerJ, 2014 Cellulosic waste represents a significant and underutilized carbon source for the biofuel industry. Owing to the recalcitrance of crystalline cellulose to enzymatic degradation, it is necessary to design economical methods of liberating the fermentable ...
Yuval Hamberg +8 more
doaj +2 more sources
Constructing an Efficient Bacillus subtilis Spore Display by Using Cohesin−Dockerin Interactions
Molecules, 2021 Bacillus subtilis spore display has become a field of increasing interest in the past two decades. To improve the efficiency of B. subtilis spore display, its directed modification was performed based on the cellulosome architecture by introducing onto ...
He Wang +3 more
doaj +1 more source
Cellulosomics, a gene-centric approach to investigating the intraspecific diversity and adaptation of Ruminococcus flavefaciens within the rumen. [PDF]
PLoS ONE, 2011 BackgroundThe bovine rumen maintains a diverse microbial community that serves to break down indigestible plant substrates. However, those bacteria specifically adapted to degrade cellulose, the major structural component of plant biomass, represent a ...
Jennifer M Brulc +13 more
doaj +1 more source
Biotechnology for Biofuels, 2021 Anaerobic fungi and methanogenic archaea are two classes of microorganisms found in the rumen microbiome that metabolically interact during lignocellulose breakdown.
Jennifer L. Brown +15 more
doaj +1 more source
Minimalistic Cellulosome of the Butanologenic Bacterium
mBio, 2020 Clostridium saccharoperbutylacetonicum is a mesophilic, anaerobic, butanol-producing bacterium, originally isolated from soil. It was recently reported that C. saccharoperbutylacetonicum possesses multiple cellulosomal elements and would potentially form
Bosmat Levi Hevroni +4 more
doaj +1 more source
Small Science, Volume 3, Issue 12, December 2023., 2023 Presenting a focused review on novel methods for protein engineering, this article delves into cutting‐edge advancements in the field of yeast surface display. Central themes include titratable avidity, on‐yeast serology, unconventional antigens including cell lysates and membrane proteins, expanded chemistry, noncanonical amino acids, and continuous ...
Joanan Lopez-Morales +8 more
wiley +1 more source