Results 11 to 20 of about 1,461 (191)

Dissection of an Anaerobic Fungal Cellulosomal Endoglucanase: Impact of the Dockerin Module on Activity, Thermostability, and Flexibility [PDF]

ACS Omega
Cellulosomal cellulases possess an extra noncatalytic module denominated dockerin, which interacts with the scaffolding via cohesion modules to organize the enzymes within the cellulosome.
Viviane Brito Andrade, Robson Tramontina, Dnane Vieira Almeida, Geizecler Tomazetto, Viviam M. da Silva, Frank Gabel, Yolanda M.B. Marcello, Ana Ligia Scott, Fabio Marcio Squina, Wanius Garcia   +9 more
doaj   +6 more sources

Cohesin-dockerin interaction in cellulosome assembly: a single Asp-to-Asn mutation disrupts high-affinity cohesin-dockerin binding

FEBS Letters, 2004
The cohesive cellulosome complex is sustained by the high-affinity cohesin–dockerin interaction. In previous work [J. Biol. Chem. 276 (2001) 9883], we demonstrated that a single Thr-to-Leu replacement in the Clostridium thermocellum dockerin component ...
Yoav Barak, Adva Mechaly, Yuval Shoham   +2 more
exaly   +4 more sources

Tripartite binding mode of cohesin-dockerin complexes from Ruminococcus flavefaciens involving naturally truncated dockerins. [PDF]

J Biol Chem
Funding Information: We acknowledge the European Synchrotron Radiation Facility (ESRF) for access to beamline ID23-EH1 of the synchrotron facility through BAG-Portugal (proposal MX-2276).
Duarte M, Carvalho AL, Ferreira MC, Caires B, Romão MJ, Prates JAM, Najmudin S, Bayer EA, Fontes CM, Bule P.   +9 more
europepmc   +6 more sources

Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome‐Based Synthetic Protein Scaffold [PDF]

Microbial Biotechnology
In systems biocatalysis, combining pathway enzymes in vitro allows for the conversion of basic substrates into more complex, valuable chemicals. However, in vitro enzyme cascades are not yet economically viable for large‐scale bio‐based chemical ...
Marte Elias, Kenan Meert, Julie Vanderstraeten, Babette Lamote, Yves Briers   +4 more
doaj   +3 more sources

Structural and functional insights into cellulosomes: masters of plant cell wall degradation [PDF]

Frontiers in Microbiology
Cellulosomes are complex multi-enzyme systems that enable efficient cellulose breakdown in some anaerobic bacteria and fungi. Understanding cellulosome functionality plays a crucial role in expanding their potential for industrial plant biomass ...
Nataša Lindič, Maša Vodovnik
doaj   +2 more sources

Study on the construction technology of β-alanine synthesizing Escherichia coli based on cellulosome assembly [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: β-Alanine is the only β-amino acid in nature; it is widely used in food additives, medicines, health products, and surfactants. To avoid pollution caused by traditional production methods, the synthesis of β-alanine has been gradually ...
Jie Lu, Jie Lu, Guodong Wang, Guodong Wang, Cuiping Yang, Cuiping Yang, Zehao Peng, Zehao Peng, Lu Yang, Lu Yang, Bowen Du, Bowen Du, Chuanzhuang Guo, Songsen Sui, Jianbin Wang, Junlin Li, Ruiming Wang, Ruiming Wang, Junqing Wang, Junqing Wang   +19 more
doaj   +2 more sources

Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin–dockerin interactions [PDF]

PeerJ, 2014
Cellulosic waste represents a significant and underutilized carbon source for the biofuel industry. Owing to the recalcitrance of crystalline cellulose to enzymatic degradation, it is necessary to design economical methods of liberating the fermentable ...
Yuval Hamberg, Vered Ruimy-Israeli, Bareket Dassa, Yoav Barak, Raphael Lamed, Kate Cameron, Carlos M.G.A. Fontes, Edward A. Bayer, Daniel B. Fried   +8 more
doaj   +3 more sources

Dual binding in cohesin-dockerin complexes: the energy landscape and the role of short, terminal segments of the dockerin module [PDF]

Scientific Reports, 2018
The assembly of the polysaccharide degradating cellulosome machinery is mediated by tight binding between cohesin and dockerin domains. We have used an empirical model known as FoldX as well as molecular mechanics methods to determine the free energy of ...
Michał Wojciechowski, Bartosz Różycki, Edward A. Bayer, Pham Dinh Quoc Huy, Marek Cieplak, Mai Suan Li   +5 more
core   +3 more sources

Functional characterization of a dockerin-containing expansin-like protein from the anaerobic fungus Neocallimastix californiae [PDF]

Biotechnology for Biofuels and Bioproducts
Background Anaerobic microbes produce multienzyme complexes known as cellulosomes to enhance the degradation of cellulosic substrates. These complexes localize diverse enzymes onto a protein scaffold, where proteins are anchored by dockerin domains ...
Taru Koitto, Anna Pohto, Elizaveta Sidorova, Thu V. Vuong, Merja Penttilä, Emma R. Master   +5 more
doaj   +2 more sources

A genomic analysis reveals the diversity of cellulosome displaying bacteria [PDF]

Frontiers in Microbiology
IntroductionSeveral species of cellulolytic bacteria display cellulosomes, massive multi-cellulase containing complexes that degrade lignocellulosic plant biomass (LCB). A greater understanding of cellulosome structure and enzyme content could facilitate
Christine M. Minor, Christine M. Minor, Allen Takayesu, Allen Takayesu, Sung Min Ha, Lukasz Salwinski, Michael R. Sawaya, Matteo Pellegrini, Robert T. Clubb, Robert T. Clubb, Robert T. Clubb   +10 more
doaj   +2 more sources