Results 41 to 50 of about 1,083 (143)

Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

BMC Genomics, 2012
Background Microbial degradation of plant cell walls and its conversion to sugars and other byproducts is a key step in the carbon cycle on Earth. In order to process heterogeneous plant-derived biomass, specialized anaerobic bacteria use an elaborate ...
Dassa Bareket, Borovok Ilya, Lamed Raphael, Henrissat Bernard, Coutinho Pedro, Hemme Christopher L, Huang Yue, Zhou Jizhong, Bayer Edward A   +8 more
doaj   +1 more source

Diversity and strain specificity of plant cell wall degrading enzymes revealed by the draft genome of Ruminococcus flavefaciens FD-1. [PDF]

PLoS ONE, 2009
BACKGROUND:Ruminococcus flavefaciens is a predominant cellulolytic rumen bacterium, which forms a multi-enzyme cellulosome complex that could play an integral role in the ability of this bacterium to degrade plant cell wall polysaccharides.
Margret E Berg Miller, Dionysios A Antonopoulos, Marco T Rincon, Mark Band, Albert Bari, Tatsiana Akraiko, Alvaro Hernandez, Jyothi Thimmapuram, Bernard Henrissat, Pedro M Coutinho, Ilya Borovok, Sadanari Jindou, Raphael Lamed, Harry J Flint, Edward A Bayer, Bryan A White   +15 more
doaj   +1 more source

Coculture with hemicellulose-fermenting microbes reverses inhibition of corn fiber solubilization by Clostridium thermocellum at elevated solids loadings

Biotechnology for Biofuels, 2021
Background The cellulolytic thermophile Clostridium thermocellum is an important biocatalyst due to its ability to solubilize lignocellulosic feedstocks without the need for pretreatment or exogenous enzyme addition. At low concentrations of substrate, C.
Dhananjay Beri, Christopher D. Herring, Sofie Blahova, Suresh Poudel, Richard J. Giannone, Robert L. Hettich, Lee R. Lynd   +6 more
doaj   +1 more source

Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis.

PLoS ONE, 2015
Degradation of cellulose is of major interest in the quest for alternative sources of renewable energy, for its positive effects on environment and ecology, and for use in advanced biotechnological applications.
Johanna Stern, Amaranta Kahn, Yael Vazana, Melina Shamshoum, Sarah Moraïs, Raphael Lamed, Edward A Bayer   +6 more
doaj   +1 more source

Integrative SAXS and AFM analysis of engineered carbohydrate‐active enzyme assemblies with tunable spatial organization

Protein Science, Volume 35, Issue 7, July 2026.
Abstract Cellulosomes are multi‐enzyme assemblies whose catalytic efficiency depends on the spatial organization of their components. However, their pronounced conformational flexibility has precluded quantitative characterization of inter‐enzyme distances and overall topology.
Iker Pardo Larrabeiti, Manuel Eibinger, Jeremy Esque, Stéphanie Pradeau, Sébastien Fort, Sarah Moraïs, Itzhak Mizrahi, Edward A. Bayer, Bernd Nidetzky, Cédric Y. Montanier, Pierre Roblin, Claire Dumon   +11 more
wiley   +1 more source

Distinctive ligand‐binding specificities of tandem PA14 biomass‐sensory elements from Clostridium thermocellum and Clostridium clariflavum

Proteins: Structure, Function, and Bioinformatics, Volume 87, Issue 11, Page 917-930, November 2019., 2019
Abstract Cellulolytic clostridia use a highly efficient cellulosome system to degrade polysaccharides. To regulate genes encoding enzymes of the multi‐enzyme cellulosome complex, certain clostridia contain alternative sigma I (σI) factors that have cognate membrane‐associated anti‐σI factors (RsgIs) which act as polysaccharide sensors. In this work, we
Inna Rozman Grinberg, Oren Yaniv, Lizett Ortiz de Ora, Iván Muñoz‐Gutiérrez, Almog Hershko, Oded Livnah, Edward A. Bayer, Ilya Borovok, Felix Frolow, Raphael Lamed, Milana Voronov‐Goldman   +10 more
wiley   +1 more source

Sporulation capability and amylosome conservation among diverse human colonic and rumen isolates of the keystone starch‐degrader Ruminococcus bromii

Environmental Microbiology, Volume 20, Issue 1, Page 324-336, January 2018., 2018
Summary Ruminococcus bromii is a dominant member of the human colonic microbiota that plays a ‘keystone’ role in degrading dietary resistant starch. Recent evidence from one strain has uncovered a unique cell surface ‘amylosome’ complex that organizes starch‐degrading enzymes.
Indrani Mukhopadhya, Sarah Moraïs, Jenny Laverde‐Gomez, Paul O. Sheridan, Alan W. Walker, William Kelly, Athol V. Klieve, Diane Ouwerkerk, Sylvia H. Duncan, Petra Louis, Nicole Koropatkin, Darrell Cockburn, Ryan Kibler, Philip J. Cooper, Carlos Sandoval, Emmanuelle Crost, Nathalie Juge, Edward A. Bayer, Harry J. Flint   +18 more
wiley   +1 more source

Creation of a functional hyperthermostable designer cellulosome

Biotechnology for Biofuels, 2019
Background Renewable energy has become a field of high interest over the past decade, and production of biofuels from cellulosic substrates has a particularly high potential as an alternative source of energy.
Amaranta Kahn, Sarah Moraïs, Anastasia P. Galanopoulou, Daehwan Chung, Nicholas S. Sarai, Neal Hengge, Dimitris G. Hatzinikolaou, Michael E. Himmel, Yannick J. Bomble, Edward A. Bayer   +9 more
doaj   +1 more source

Intramolecular clasp of the cellulosomal Ruminococcus flavefaciens ScaA dockerin module confers structural stability

FEBS Open Bio, Volume 3, Issue 1, Page 398-405, January 01, 2013., 2013
A structural model for the Ruminococcus flavefaciens ScaA dockerin is proposed. A stacking interaction between N‐ and C‐terminal residues was derived from the model. Mutations of putative interacting residues resulted in reduced stability and binding. Similar intramodular “clasp” interactions were observed in other dockerin structures.
Michal Slutzki, Maroor K. Jobby, Seth Chitayat, Alon Karpol, Bareket Dassa, Yoav Barak, Raphael Lamed, Steven P. Smith, Edward A. Bayer   +8 more
wiley   +1 more source

Bioactive polysaccharides and their potential health benefits in reducing the risks of metabolic syndrome: A review

Food Biomacromolecules, Volume 2, Issue 3, Page 288-314, September 2025.
Abstract Metabolic syndrome (MetS) is a complex noncommunicable condition characterized by central obesity, insulin resistance, hyperlipidemia, hypertension, and hyperglycemia. Epidemiological evidence shows that MetS affects a third of the global population; however, due to its complex pathogenesis and limited drug therapy options, the increasing ...
Josiah Owuor Oyalo, Evelyne Nkirote Gikundi, Mercy Adhiambo Ndiege, Kevin Omondi Aduol   +3 more
wiley   +1 more source