Results 41 to 50 of about 1,033 (184)

A Scaffoldin of the Bacteroides cellulosolvens Cellulosome That Contains 11 Type II Cohesins [PDF]

Journal of Bacteriology, 2000
ABSTRACT A cellulosomal scaffoldin gene, termed cipBc , was identified and sequenced from the mesophilic cellulolytic anaerobe Bacteroides cellulosolvens . The gene encodes a 2,292-residue polypeptide (excluding the signal sequence) with a calculated molecular weight of 242,437 ...
S Y, Ding, E A, Bayer, D, Steiner, Y, Shoham, R, Lamed   +4 more
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The Cellulosome Paradigm in An Extreme Alkaline Environment

Microorganisms, 2019
Rapid decomposition of plant biomass in soda lakes is associated with microbial activity of anaerobic cellulose-degrading communities. The alkaliphilic bacterium, Clostridium alkalicellulosi, is the single known isolate from a soda lake that demonstrates
Paripok Phitsuwan, Sarah Moraïs, Bareket Dassa, Bernard Henrissat, Edward A. Bayer   +4 more
doaj   +1 more source

In vivo Functional Characterization of Hydrophilic X2 Modules in the Cellulosomal Scaffolding Protein

Frontiers in Microbiology, 2022
As part of free cellulases or scaffolding proteins in cellulosomes, the hydrophilic non-catalytic X2 module is widely distributed in cellulolytic Clostridia or other Firmicutes bacteria. Previous biochemical studies suggest that X2 modules might increase
Xuanyu Tao, Jiantao Liu, Jiantao Liu, Megan L. Kempher, Tao Xu, Tao Xu, Tao Xu, Jizhong Zhou, Jizhong Zhou   +8 more
doaj   +1 more source

Characterization and subcellular localization of the Clostridium thermocellum scaffoldin dockerin binding protein SdbA [PDF]

Journal of Bacteriology, 1997
This article reports the characterization of the Clostridium thermocellum SdbA protein thought to anchor the cellulosome to the bacterial cell surface. The NH2-terminal region of SdbA consists of a cohesin domain which specifically binds the dockerin domain of the cellulosomal scaffolding protein CipA. The COOH-terminal region consists of a triplicated
E, Leibovitz, H, Ohayon, P, Gounon, P, Béguin   +3 more
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Designing chimeric enzymes inspired by fungal cellulosomes

Synthetic and Systems Biotechnology, 2020
Cellulosomes are synthesized by anaerobic bacteria and fungi to degrade lignocellulose via synergistic action of multiple enzymes fused to a protein scaffold.
Sean P. Gilmore, Stephen P. Lillington, Charles H. Haitjema, Randall de Groot, Michelle A. O'Malley   +4 more
doaj   +1 more source

Controlled Aggregation and Increased Stability of β-Glucuronidase by Cellulose Binding Domain Fusion. [PDF]

PLoS ONE, 2017
Cellulose-binding domains (CBDs) are protein domains with cellulose-binding activity, and some act as leaders in the localization of cellulosomal scaffoldin proteins to the hydrophobic surface of crystalline cellulose.
Soo-Jin Yeom, Gui Hwan Han, Moonjung Kim, Kil Koang Kwon, Yaoyao Fu, Haseong Kim, Hyewon Lee, Dae-Hee Lee, Heungchae Jung, Seung-Goo Lee   +9 more
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Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme

Microorganisms, 2017
The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides.
Bareket Dassa, Ilya Borovok, Vincent Lombard, Bernard Henrissat, Raphael Lamed, Edward A. Bayer, Sarah Moraïs   +6 more
doaj   +1 more source

Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar

Nature Communications, 2023
The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both
Yayu Guo, Shufang Wang, Keji Yu, Hou-Ling Wang, Huimin Xu, Chengwei Song, Yuanyuan Zhao, Jialong Wen, Chunxiang Fu, Yu Li, Shuizhong Wang, Xi Zhang, Yan Zhang, Yuan Cao, Fenjuan Shao, Xiaohua Wang, Xin Deng, Tong Chen, Qiao Zhao, Lei Li, Guodong Wang, Paul Grünhofer, Lukas Schreiber, Yue Li, Guoyong Song, Richard A. Dixon, Jinxing Lin   +26 more
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Transcriptome and Zymogram Analyses Reveal a Cellobiose-Dose Related Reciprocal Regulatory Effect on Cellulase Synthesis in Cellulosilyticum ruminicola H1

Frontiers in Microbiology, 2017
The rumen bacterium Cellulosilyticum ruminicola H1 efficiently hydrolyzes cellulose. To gain insights into the regulatory mechanisms of cellulase synthesis, comparative transcriptome analysis was conducted for cultures grown on 2% filter paper, 0.5 and 0.
Shanzhen Li, Shanzhen Li, Nana Shao, Nana Shao, Yuanming Luo, Hongcan Liu, Shichun Cai, Xiuzhu Dong   +7 more
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Structure of CBM3b of the major cellulosomal scaffoldin subunit ScaA fromAcetivibrio cellulolyticus [PDF]

Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2011
The carbohydrate-binding module (CBM) of the major scaffoldin subunit ScaA of the cellulosome of Acetivibrio cellulolyticus is classified as a family 3b CBM and binds strongly to cellulose. The CBM3b was overexpressed, purified and crystallized, and its three-dimensional structure was determined. The structure contained a nickel-binding site located at
Oren, Yaniv, Yehuda, Halfon, Linda J W, Shimon, Edward A, Bayer, Raphael, Lamed, Felix, Frolow   +5 more
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