Results 61 to 70 of about 1,033 (184)

Distinctive ligand‐binding specificities of tandem PA14 biomass‐sensory elements from Clostridium thermocellum and Clostridium clariflavum

Proteins: Structure, Function, and Bioinformatics, Volume 87, Issue 11, Page 917-930, November 2019., 2019
Abstract Cellulolytic clostridia use a highly efficient cellulosome system to degrade polysaccharides. To regulate genes encoding enzymes of the multi‐enzyme cellulosome complex, certain clostridia contain alternative sigma I (σI) factors that have cognate membrane‐associated anti‐σI factors (RsgIs) which act as polysaccharide sensors. In this work, we
Inna Rozman Grinberg, Oren Yaniv, Lizett Ortiz de Ora, Iván Muñoz‐Gutiérrez, Almog Hershko, Oded Livnah, Edward A. Bayer, Ilya Borovok, Felix Frolow, Raphael Lamed, Milana Voronov‐Goldman   +10 more
wiley   +1 more source

Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis.

PLoS ONE, 2015
Degradation of cellulose is of major interest in the quest for alternative sources of renewable energy, for its positive effects on environment and ecology, and for use in advanced biotechnological applications.
Johanna Stern, Amaranta Kahn, Yael Vazana, Melina Shamshoum, Sarah Moraïs, Raphael Lamed, Edward A Bayer   +6 more
doaj   +1 more source

Sporulation capability and amylosome conservation among diverse human colonic and rumen isolates of the keystone starch‐degrader Ruminococcus bromii

Environmental Microbiology, Volume 20, Issue 1, Page 324-336, January 2018., 2018
Summary Ruminococcus bromii is a dominant member of the human colonic microbiota that plays a ‘keystone’ role in degrading dietary resistant starch. Recent evidence from one strain has uncovered a unique cell surface ‘amylosome’ complex that organizes starch‐degrading enzymes.
Indrani Mukhopadhya, Sarah Moraïs, Jenny Laverde‐Gomez, Paul O. Sheridan, Alan W. Walker, William Kelly, Athol V. Klieve, Diane Ouwerkerk, Sylvia H. Duncan, Petra Louis, Nicole Koropatkin, Darrell Cockburn, Ryan Kibler, Philip J. Cooper, Carlos Sandoval, Emmanuelle Crost, Nathalie Juge, Edward A. Bayer, Harry J. Flint   +18 more
wiley   +1 more source

Intramolecular clasp of the cellulosomal Ruminococcus flavefaciens ScaA dockerin module confers structural stability

FEBS Open Bio, Volume 3, Issue 1, Page 398-405, January 01, 2013., 2013
A structural model for the Ruminococcus flavefaciens ScaA dockerin is proposed. A stacking interaction between N‐ and C‐terminal residues was derived from the model. Mutations of putative interacting residues resulted in reduced stability and binding. Similar intramodular “clasp” interactions were observed in other dockerin structures.
Michal Slutzki, Maroor K. Jobby, Seth Chitayat, Alon Karpol, Bareket Dassa, Yoav Barak, Raphael Lamed, Steven P. Smith, Edward A. Bayer   +8 more
wiley   +1 more source

Architecture of the Bacteroides cellulosolvens Cellulosome: Description of a Cell Surface-Anchoring Scaffoldin and a Family 48 Cellulase [PDF]

Journal of Bacteriology, 2004
ABSTRACT A large gene downstream of the primary Bacteroides cellulosolvens cellulosomal scaffoldin ( cipBc , now renamed scaA ) was sequenced. The gene, termed scaB , contained an N-terminal leader peptide followed by 10 type I ...
Qi, Xu, Edward A, Bayer, Milana, Goldman, Rina, Kenig, Yuval, Shoham, Raphael, Lamed   +5 more
openaire   +2 more sources

Creation of a functional hyperthermostable designer cellulosome

Biotechnology for Biofuels, 2019
Background Renewable energy has become a field of high interest over the past decade, and production of biofuels from cellulosic substrates has a particularly high potential as an alternative source of energy.
Amaranta Kahn, Sarah Moraïs, Anastasia P. Galanopoulou, Daehwan Chung, Nicholas S. Sarai, Neal Hengge, Dimitris G. Hatzinikolaou, Michael E. Himmel, Yannick J. Bomble, Edward A. Bayer   +9 more
doaj   +1 more source

Bioactive polysaccharides and their potential health benefits in reducing the risks of metabolic syndrome: A review

Food Biomacromolecules, Volume 2, Issue 3, Page 288-314, September 2025.
Abstract Metabolic syndrome (MetS) is a complex noncommunicable condition characterized by central obesity, insulin resistance, hyperlipidemia, hypertension, and hyperglycemia. Epidemiological evidence shows that MetS affects a third of the global population; however, due to its complex pathogenesis and limited drug therapy options, the increasing ...
Josiah Owuor Oyalo, Evelyne Nkirote Gikundi, Mercy Adhiambo Ndiege, Kevin Omondi Aduol   +3 more
wiley   +1 more source

Single Binding Mode Integration of Hemicellulose-degrading Enzymes via Adaptor Scaffoldins in Ruminococcus flavefaciens Cellulosome [PDF]

Journal of Biological Chemistry, 2016
The assembly of one of Nature's most elaborate multienzyme complexes, the cellulosome, results from the binding of enzyme-borne dockerins to reiterated cohesin domains located in a non-catalytic primary scaffoldin. Generally, dockerins present two similar cohesin-binding interfaces that support a dual binding mode. The dynamic integration of enzymes in
Bule P, Alves VD, Leitao A, Ferreira LMA, Bayer EA, Smith SP, Gilbert HJ, Najmudin S, Fontes CMGA   +8 more
openaire   +3 more sources

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction [PDF]

, 2018
\ua9 2018 The Author(s). Cellulosomes are highly sophisticated molecular nanomachines that participate in the deconstruction of complex polysaccharides, notably cellulose and hemicellulose.
Pires VMR, Carvalho AL, Fontes CMGA, Smith SP, Gilbert HJ, Prates JAM, Najmudin S, Alves VD, Noach I, Ferreira LMA, Bayer EA, Bule P   +11 more
core  

Magnetizing Biotech–Advances in (In Vivo) Magnetic Enzyme Immobilization

Engineering in Life Sciences, Volume 25, Issue 3, March 2025.
ABSTRACT Industrial biocatalysis, a multibillion dollar industry, relies on the selectivity and efficacy of enzymes for efficient chemical transformations. However, enzymes, evolutionary adapted to mild biological conditions, often struggle in industrial processes that require harsh reaction conditions, resulting in reduced stability and activity ...
Gizem Ölçücü, Karl‐Erich Jaeger, Ulrich Krauss   +2 more
wiley   +1 more source