Results 61 to 70 of about 754 (153)

Architecture of the Bacteroides cellulosolvens Cellulosome: Description of a Cell Surface-Anchoring Scaffoldin and a Family 48 Cellulase [PDF]

Journal of Bacteriology, 2004
ABSTRACT A large gene downstream of the primary Bacteroides cellulosolvens cellulosomal scaffoldin ( cipBc , now renamed scaA ) was sequenced. The gene, termed scaB , contained an N-terminal leader peptide followed by 10 type I ...
Qi, Xu, Edward A, Bayer, Milana, Goldman, Rina, Kenig, Yuval, Shoham, Raphael, Lamed   +5 more
openaire   +2 more sources

Intramolecular clasp of the cellulosomal Ruminococcus flavefaciens ScaA dockerin module confers structural stability

FEBS Open Bio, Volume 3, Issue 1, Page 398-405, January 01, 2013., 2013
A structural model for the Ruminococcus flavefaciens ScaA dockerin is proposed. A stacking interaction between N‐ and C‐terminal residues was derived from the model. Mutations of putative interacting residues resulted in reduced stability and binding. Similar intramodular “clasp” interactions were observed in other dockerin structures.
Michal Slutzki, Maroor K. Jobby, Seth Chitayat, Alon Karpol, Bareket Dassa, Yoav Barak, Raphael Lamed, Steven P. Smith, Edward A. Bayer   +8 more
wiley   +1 more source

Creation of a functional hyperthermostable designer cellulosome

Biotechnology for Biofuels, 2019
Background Renewable energy has become a field of high interest over the past decade, and production of biofuels from cellulosic substrates has a particularly high potential as an alternative source of energy.
Amaranta Kahn, Sarah Moraïs, Anastasia P. Galanopoulou, Daehwan Chung, Nicholas S. Sarai, Neal Hengge, Dimitris G. Hatzinikolaou, Michael E. Himmel, Yannick J. Bomble, Edward A. Bayer   +9 more
doaj   +1 more source

Bioactive polysaccharides and their potential health benefits in reducing the risks of metabolic syndrome: A review

Food Biomacromolecules, Volume 2, Issue 3, Page 288-314, September 2025.
Abstract Metabolic syndrome (MetS) is a complex noncommunicable condition characterized by central obesity, insulin resistance, hyperlipidemia, hypertension, and hyperglycemia. Epidemiological evidence shows that MetS affects a third of the global population; however, due to its complex pathogenesis and limited drug therapy options, the increasing ...
Josiah Owuor Oyalo, Evelyne Nkirote Gikundi, Mercy Adhiambo Ndiege, Kevin Omondi Aduol   +3 more
wiley   +1 more source

Single Binding Mode Integration of Hemicellulose-degrading Enzymes via Adaptor Scaffoldins in Ruminococcus flavefaciens Cellulosome [PDF]

Journal of Biological Chemistry, 2016
The assembly of one of Nature's most elaborate multienzyme complexes, the cellulosome, results from the binding of enzyme-borne dockerins to reiterated cohesin domains located in a non-catalytic primary scaffoldin. Generally, dockerins present two similar cohesin-binding interfaces that support a dual binding mode. The dynamic integration of enzymes in
Bule P, Alves VD, Leitao A, Ferreira LMA, Bayer EA, Smith SP, Gilbert HJ, Najmudin S, Fontes CMGA   +8 more
openaire   +3 more sources

Magnetizing Biotech–Advances in (In Vivo) Magnetic Enzyme Immobilization

Engineering in Life Sciences, Volume 25, Issue 3, March 2025.
ABSTRACT Industrial biocatalysis, a multibillion dollar industry, relies on the selectivity and efficacy of enzymes for efficient chemical transformations. However, enzymes, evolutionary adapted to mild biological conditions, often struggle in industrial processes that require harsh reaction conditions, resulting in reduced stability and activity ...
Gizem Ölçücü, Karl‐Erich Jaeger, Ulrich Krauss   +2 more
wiley   +1 more source

A modular enzyme with combined hemicellulose‐removing and LPMO activity increases cellulose accessibility in softwood

The FEBS Journal, Volume 292, Issue 1, Page 75-93, January 2025.
This study describes a unique bi‐catalytic enzyme that combines an oxidative activity (LPMO) acting on cellulose and a hydrolase activity (Man5) acting on mannan thus facilitating the degradation of complex, co‐polymeric plant cell walls. The enzyme works by removing the most abundant cellulose‐covering softwood hemicellulose, mannan, which then allows
Zarah Forsberg, Tina R. Tuveng, Vincent G. H. Eijsink   +2 more
wiley   +1 more source

Optimizing the composition of a synthetic cellulosome complex for the hydrolysis of softwood pulp: identification of the enzymatic core functions and biochemical complex characterization

Biotechnology for Biofuels, 2018
Background The development of efficient cellulase blends is a key factor for cost-effectively valorizing biomass in a new bio-economy. Today, the enzymatic hydrolysis of plant-derived polysaccharides is mainly accomplished with fungal cellulases, whereas
Benedikt Leis, Claudia Held, Björn Andreeßen, Wolfgang Liebl, Sigrid Graubner, Louis-Philipp Schulte, Wolfgang H. Schwarz, Vladimir V. Zverlov   +7 more
doaj   +1 more source

Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis

Biotechnology for Biofuels, 2018
Background Enzymatic conversion of lignocellulosic biomass into soluble sugars is a major bottleneck in the plant biomass utilization. Several anaerobic organisms cope these issues via multiple-enzyme complex system so called ‘cellulosome’.
Jui-Jen Chang, Marimuthu Anandharaj, Cheng-Yu Ho, Kenji Tsuge, Tsung-Yu Tsai, Huei-Mien Ke, Yu-Ju Lin, Minh Dung Ha Tran, Wen-Hsiung Li, Chieh-Chen Huang   +9 more
doaj   +1 more source

Highly active spore biocatalyst by self‐assembly of co‐expressed anchoring scaffoldin and multimeric enzyme

Biotechnology and Bioengineering, 2017
AbstractWe report a spore‐based biocatalysis platform capable of producing and self‐assembling active multimeric enzymes on a spore surface with a high loading density. This was achieved by co‐expressing both a spore surface‐anchoring scaffoldin protein containing multiple cohesin domains and a dockerin‐tagged enzyme of interest in the mother cell ...
Long Chen, Megan Holmes, Elise Schaefer, Ashok Mulchandani, Xin Ge   +4 more
openaire   +3 more sources