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The FEBS Journal, Volume 292, Issue 1, Page 75-93, January 2025.This study describes a unique bi‐catalytic enzyme that combines an oxidative activity (LPMO) acting on cellulose and a hydrolase activity (Man5) acting on mannan thus facilitating the degradation of complex, co‐polymeric plant cell walls. The enzyme works by removing the most abundant cellulose‐covering softwood hemicellulose, mannan, which then allows
Zarah Forsberg +2 more
wiley +1 more source
Biotechnology for Biofuels, 2018 Background The development of efficient cellulase blends is a key factor for cost-effectively valorizing biomass in a new bio-economy. Today, the enzymatic hydrolysis of plant-derived polysaccharides is mainly accomplished with fungal cellulases, whereas
Benedikt Leis +7 more
doaj +1 more source
, 2016 Additional file 3. Thermostability of conventional versus thermostable designer cellulosomes and components. Non-denaturing PAGE (9Â %) was employed for assessing total complex formation of the thermostable chimaeric scaffoldin and the wild-type or ...
Edward Bayer (3405971) +9 more
core +1 more source
, 1955 McClintic-Marshal exterior - construction workers, construction equipment ...
core +2 more sources
Biotechnology for Biofuels, 2018 Background Enzymatic conversion of lignocellulosic biomass into soluble sugars is a major bottleneck in the plant biomass utilization. Several anaerobic organisms cope these issues via multiple-enzyme complex system so called ‘cellulosome’.
Jui-Jen Chang +9 more
doaj +1 more source
Biotechnology and Bioengineering, 2017 AbstractWe report a spore‐based biocatalysis platform capable of producing and self‐assembling active multimeric enzymes on a spore surface with a high loading density. This was achieved by co‐expressing both a spore surface‐anchoring scaffoldin protein containing multiple cohesin domains and a dockerin‐tagged enzyme of interest in the mother cell ...
Long Chen +4 more
openaire +3 more sources
Self-Assembled Amyloid-Like Oligomeric-Cohesin Scaffoldin for Augmented Protein Display on the Saccharomyces cerevisiae Cell Surface [PDF]
Applied and Environmental Microbiology, 2012 ABSTRACT In this study, a molecular self-assembly strategy to develop a novel protein scaffold for amplifying the extent and variety of proteins displayed on the surface of Saccharomyces cerevisiae is presented.
Zhenlin, Han +4 more
openaire +2 more sources
Biotechnology for Biofuels, 2017 Background Bioethanol production processes involve enzymatic hydrolysis of pretreated lignocellulosic biomass into fermentable sugars. Due to the relatively high cost of enzyme production, the development of potent and cost-effective cellulolytic ...
Shahar Yoav +8 more
doaj +1 more source
The trench dug for the bluff balustrade filled with scaffolding
, 2022 The trench dug for the bluff balustrade filled with ...
core
Biotechnology for Biofuels, 2019 Background (Pseudo)Bacteroides cellulosolvens is a cellulolytic bacterium that produces the most extensive and intricate cellulosomal system known in nature. Recently, the elaborate architecture of the B.
Olga Zhivin-Nissan +5 more
doaj +1 more source