Results 71 to 80 of about 754 (153)

Self-Assembled Amyloid-Like Oligomeric-Cohesin Scaffoldin for Augmented Protein Display on the Saccharomyces cerevisiae Cell Surface [PDF]

Applied and Environmental Microbiology, 2012
ABSTRACT In this study, a molecular self-assembly strategy to develop a novel protein scaffold for amplifying the extent and variety of proteins displayed on the surface of Saccharomyces cerevisiae is presented.
Zhenlin, Han, Bei, Zhang, Yi E, Wang, Yi Y, Zuo, Wei Wen, Su   +4 more
openaire   +2 more sources

How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313?

Biotechnology for Biofuels, 2017
Background Bioethanol production processes involve enzymatic hydrolysis of pretreated lignocellulosic biomass into fermentable sugars. Due to the relatively high cost of enzyme production, the development of potent and cost-effective cellulolytic ...
Shahar Yoav, Yoav Barak, Melina Shamshoum, Ilya Borovok, Raphael Lamed, Bareket Dassa, Yitzhak Hadar, Ely Morag, Edward A. Bayer   +8 more
doaj   +1 more source

Unraveling essential cellulosomal components of the (Pseudo)Bacteroides cellulosolvens reveals an extensive reservoir of novel catalytic enzymes

Biotechnology for Biofuels, 2019
Background (Pseudo)Bacteroides cellulosolvens is a cellulolytic bacterium that produces the most extensive and intricate cellulosomal system known in nature. Recently, the elaborate architecture of the B.
Olga Zhivin-Nissan, Bareket Dassa, Ely Morag, Meital Kupervaser, Yishai Levin, Edward A. Bayer   +5 more
doaj   +1 more source

Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase

Microbial Cell Factories, 2013
Background Cellulosic biomass is considered as a promising alternative to fossil fuels, but its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this material.
Kim Sujin, Baek Seung-Ho, Lee Kyusung, Hahn Ji-Sook   +3 more
doaj   +1 more source

Beyond enzyme engineering: ordered enzyme immobilization drives enhanced productivity in vitro

Frontiers in Bioengineering and Biotechnology
Increasing environmental and economic pressures associated with global fossil fuel demand necessitate a shift toward sustainable fuel production. Production of second-generation biofuels, such as isobutanol, presents a promising opportunity; however ...
Matthew Wong, Thomas G. Neuman, Md Anarul Hoque, Sarah Moraïs, Edward Bayer, Edward Bayer, Georges Belfort, Mattheos Koffas   +7 more
doaj   +1 more source

Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis

Microbial Cell Factories, 2010
Background The assembly and spatial organization of enzymes in naturally occurring multi-protein complexes is of paramount importance for the efficient degradation of complex polymers and biosynthesis of valuable products.
Wieczorek Andrew S, Martin Vincent JJ
doaj   +1 more source

Clostridium clariflavum: Key Cellulosome Players Are Revealed by Proteomic Analysis

mBio, 2015
Clostridium clariflavum is an anaerobic, cellulosome-forming thermophile, containing in its genome genes for a large number of cellulosomal enzyme and a complex scaffoldin system.
Lior Artzi, Ely Morag, Yoav Barak, Raphael Lamed, Edward A. Bayer   +4 more
doaj   +1 more source

Deconstruction of Lignocellulose into Soluble Sugars by Native and Designer Cellulosomes

mBio, 2012
Lignocellulosic biomass, the most abundant polymer on Earth, is typically composed of three major constituents: cellulose, hemicellulose, and lignin. The crystallinity of cellulose, hydrophobicity of lignin, and encapsulation of cellulose by the lignin ...
Sarah Moraïs, Ely Morag, Yoav Barak, Dan Goldman, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, Edward A. Bayer   +8 more
doaj   +1 more source

Unique Organization of Extracellular Amylases into Amylosomes in the Resistant Starch-Utilizing Human Colonic Firmicutes Bacterium Ruminococcus bromii

mBio, 2015
Ruminococcus bromii is a dominant member of the human gut microbiota that plays a key role in releasing energy from dietary starches that escape digestion by host enzymes via its exceptional activity against particulate “resistant” starches.
Xiaolei Ze, Yonit Ben David, Jenny A. Laverde-Gomez, Bareket Dassa, Paul O. Sheridan, Sylvia H. Duncan, Petra Louis, Bernard Henrissat, Nathalie Juge, Nicole M. Koropatkin, Edward A. Bayer, Harry J. Flint   +11 more
doaj   +1 more source

Impact of pretreated Switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis.

PLoS ONE, 2009
BackgroundEconomic feasibility and sustainability of lignocellulosic ethanol production requires the development of robust microorganisms that can efficiently degrade and convert plant biomass to ethanol.
Babu Raman, Chongle Pan, Gregory B Hurst, Miguel Rodriguez, Catherine K McKeown, Patricia K Lankford, Nagiza F Samatova, Jonathan R Mielenz   +7 more
doaj   +1 more source