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Microbial Biotechnology, Volume 18, Issue 4, April 2025.The first three Escherichia coli glycolytic enzymes were converted to the docking enzyme mode and subsequently scaffolded. Increasing copies of the rate‐limiting enzyme boosted the overall performance. At lower enzyme densities, the proximity effects provided by scaffold‐colocalisation result in a higher product yield compared to free docking enzymes ...
Marte Elias +4 more
wiley +1 more source
Magnetizing Biotech–Advances in (In Vivo) Magnetic Enzyme Immobilization
Engineering in Life Sciences, Volume 25, Issue 3, March 2025.ABSTRACT Industrial biocatalysis, a multibillion dollar industry, relies on the selectivity and efficacy of enzymes for efficient chemical transformations. However, enzymes, evolutionary adapted to mild biological conditions, often struggle in industrial processes that require harsh reaction conditions, resulting in reduced stability and activity ...
Gizem Ölçücü +2 more
wiley +1 more source
The FEBS Journal, Volume 292, Issue 1, Page 75-93, January 2025.This study describes a unique bi‐catalytic enzyme that combines an oxidative activity (LPMO) acting on cellulose and a hydrolase activity (Man5) acting on mannan thus facilitating the degradation of complex, co‐polymeric plant cell walls. The enzyme works by removing the most abundant cellulose‐covering softwood hemicellulose, mannan, which then allows
Zarah Forsberg +2 more
wiley +1 more source
, 2016 Additional file 3. Thermostability of conventional versus thermostable designer cellulosomes and components. Non-denaturing PAGE (9Â %) was employed for assessing total complex formation of the thermostable chimaeric scaffoldin and the wild-type or ...
Edward Bayer (3405971) +9 more
core +1 more source
Biotechnology for Biofuels, 2018 Background The development of efficient cellulase blends is a key factor for cost-effectively valorizing biomass in a new bio-economy. Today, the enzymatic hydrolysis of plant-derived polysaccharides is mainly accomplished with fungal cellulases, whereas
Benedikt Leis +7 more
doaj +1 more source
Biotechnology for Biofuels, 2018 Background Enzymatic conversion of lignocellulosic biomass into soluble sugars is a major bottleneck in the plant biomass utilization. Several anaerobic organisms cope these issues via multiple-enzyme complex system so called ‘cellulosome’.
Jui-Jen Chang +9 more
doaj +1 more source
Biotechnology for Biofuels, 2017 Background Bioethanol production processes involve enzymatic hydrolysis of pretreated lignocellulosic biomass into fermentable sugars. Due to the relatively high cost of enzyme production, the development of potent and cost-effective cellulolytic ...
Shahar Yoav +8 more
doaj +1 more source
, 1955 McClintic-Marshal exterior - construction workers, construction equipment ...
core +2 more sources
Biotechnology for Biofuels, 2019 Background (Pseudo)Bacteroides cellulosolvens is a cellulolytic bacterium that produces the most extensive and intricate cellulosomal system known in nature. Recently, the elaborate architecture of the B.
Olga Zhivin-Nissan +5 more
doaj +1 more source
Microbial Cell Factories, 2013 Background Cellulosic biomass is considered as a promising alternative to fossil fuels, but its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this material.
Kim Sujin +3 more
doaj +1 more source