The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons [PDF]
Biotechnology for Biofuels, 2014 Clostridium thermocellum is a thermophilic anaerobic bacterium that degrades cellulose by using a highly effective cellulosome, a macromolecular complex consisting of multiple cellulose degrading enzymes organized and attached to the cell surface by non-catalytic scaffoldins. However, due largely to lack of efficient methods for genetic manipulation of
Hong, Wei +7 more
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Efficient yeast surface-display of novel complex synthetic cellulosomes
Microbial Cell Factories, 2018 Background The self-assembly of cellulosomes on the surface of yeast is a promising strategy for consolidated bioprocessing to convert cellulose into ethanol in one step.
Hongting Tang +6 more
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The Cellulosome Paradigm in An Extreme Alkaline Environment
Microorganisms, 2019 Rapid decomposition of plant biomass in soda lakes is associated with microbial activity of anaerobic cellulose-degrading communities. The alkaliphilic bacterium, Clostridium alkalicellulosi, is the single known isolate from a soda lake that demonstrates
Paripok Phitsuwan +4 more
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Designing chimeric enzymes inspired by fungal cellulosomes
Synthetic and Systems Biotechnology, 2020 Cellulosomes are synthesized by anaerobic bacteria and fungi to degrade lignocellulose via synergistic action of multiple enzymes fused to a protein scaffold.
Sean P. Gilmore +4 more
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Controlled Aggregation and Increased Stability of β-Glucuronidase by Cellulose Binding Domain Fusion. [PDF]
PLoS ONE, 2017 Cellulose-binding domains (CBDs) are protein domains with cellulose-binding activity, and some act as leaders in the localization of cellulosomal scaffoldin proteins to the hydrophobic surface of crystalline cellulose.
Soo-Jin Yeom +9 more
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Frontiers in Microbiology, 2022 As part of free cellulases or scaffolding proteins in cellulosomes, the hydrophilic non-catalytic X2 module is widely distributed in cellulolytic Clostridia or other Firmicutes bacteria. Previous biochemical studies suggest that X2 modules might increase
Xuanyu Tao +8 more
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A Scaffoldin of the Bacteroides cellulosolvens Cellulosome That Contains 11 Type II Cohesins [PDF]
Journal of Bacteriology, 2000 ABSTRACT A cellulosomal scaffoldin gene, termed cipBc , was identified and sequenced from the mesophilic cellulolytic anaerobe Bacteroides cellulosolvens . The gene encodes a 2,292-residue polypeptide (excluding the signal sequence) with a calculated molecular weight of 242,437 ...
S Y, Ding +4 more
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Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme
Microorganisms, 2017 The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides.
Bareket Dassa +6 more
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Characterization and subcellular localization of the Clostridium thermocellum scaffoldin dockerin binding protein SdbA [PDF]
Journal of Bacteriology, 1997 This article reports the characterization of the Clostridium thermocellum SdbA protein thought to anchor the cellulosome to the bacterial cell surface. The NH2-terminal region of SdbA consists of a cohesin domain which specifically binds the dockerin domain of the cellulosomal scaffolding protein CipA. The COOH-terminal region consists of a triplicated
E, Leibovitz +3 more
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Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar
Nature Communications, 2023 The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both
Yayu Guo +26 more
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