Results 21 to 30 of about 754 (153)

Engineering and characterization of carbohydrate‐binding modules for imaging cellulose fibrils biosynthesis in plant protoplasts

Biotechnology and Bioengineering, Volume 120, Issue 8, Page 2253-2268, August 2023., 2023
Carbohydrate binding modules (CBMs) are noncatalytic domains that have been used to visualize distinct polysaccharides present in the plant cell wall. Here, engineered type‐A tandem CBMs have been characterized using pull‐down and kinetic assays.
Dharanidaran Jayachandran, Peter Smith, Mohammad Irfan, Junhong Sun, John M. Yarborough, Yannick J. Bomble, Eric Lam, Shishir P. S. Chundawat   +7 more
wiley   +1 more source

Immunolocalization of Scaffoldin, a Trichohyalin‐Like Protein, in the Epidermis of the Chicken Embryo [PDF]

The Anatomical Record, 2014
ABSTRACTRecent comparative genomic studies have identified a chicken gene that codes for a trichohyalin‐like protein rich in arginine and glutamic acid termed scaffoldin. Immunocytochemistry and immunoelectron microscopy show that this protein is predominantly localized in periderm granules, subcellular structures present in the periderm of the ...
L, Alibardi, V, Mlitz, L, Eckhart
openaire   +2 more sources

Synthetic Multienzyme Assemblies for Natural Product Biosynthesis

ChemBioChem, Volume 24, Issue 6, March 14, 2023., 2023
Constructing multienzyme complexes has become an effective way to optimize metabolic flux and increase the titer of natural products in metabolic engineering recently. In this review, we summarize strategies for constructing synthetic multienzyme complexes in microbial cells and their applications in promoting natural product biosynthesis.
Min Liu, Yue Wang, Hao Jiang, Yongxu Han, Jiang Xia   +4 more
wiley   +1 more source

Regulation of Expression of Scaffoldin-Related Genes in Clostridium thermocellum [PDF]

Journal of Bacteriology, 2003
ABSTRACT Clostridium thermocellum produces an extracellular multienzyme complex, termed the cellulosome, that allows efficient solubilization of crystalline cellulose. The complex is organized around a large noncatalytic protein subunit, termed CipA or scaffoldin, and is found either free in the supernatant or cell ...
Tali W, Dror, Adi, Rolider, Edward A, Bayer, Raphael, Lamed, Yuval, Shoham   +4 more
openaire   +2 more sources

Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing

Microbial Biotechnology, Volume 16, Issue 2, Page 238-261, February 2023., 2023
Development of consolidated bioprocessing (CBP) of lignocellulose to butanol can potentially replace butanol production from non‐renewable sources at viable costs. Progress in CBP development has been obtained by combination of (hemi)cellulolytic and butanol‐producing features in a single strain (by metabolic engineering or protoplast fusion) or by ...
Angela Re, Roberto Mazzoli
wiley   +1 more source

Production of Heterologous and Chimeric Scaffoldins by Clostridium acetobutylicum ATCC 824 [PDF]

Journal of Bacteriology, 2004
ABSTRACT Clostridium acetobutylicum ATCC 824 converts sugars and various polysaccharides into acids and solvents. This bacterium, however, is unable to utilize cellulosic substrates, since it is able to secrete very small amounts of cellulosomes.
Perret, S., /Casalot, Laurence, Fierobe, H.P., Tardif, C., Sabathe, F., Belaich, J.P., Belaich, A.   +6 more
openaire   +3 more sources

Prevalent association with the bacterial cell envelope of prokaryotic expansins revealed by bioinformatics analysis

Protein Science, Volume 31, Issue 5, May 2022., 2022
Abstract Expansins are a group of proteins from diverse organisms from bacteria to plants. Although expansins show structural conservation, their biological roles seem to differ among kingdoms. In plants, these proteins remodel the cell wall during plant growth and other processes.
Andrés de Sandozequi, Juan José Salazar‐Cortés, Irán Tapia‐Vázquez, Claudia Martínez‐Anaya   +3 more
wiley   +1 more source

Comparative Transcriptomics Reveals a Dual Role of the Epidermal Differentiation Complex in the Skin and the Oesophagus. [PDF]

Exp Dermatol
ABSTRACT The epidermal differentiation complex (EDC) is a cluster of genes implicated in the control of the skin barrier. However, some EDC genes are also expressed at high levels in the human oesophagus. To determine whether the expression of EDC genes in the oesophagus is evolutionarily conserved, we performed comparative transcriptomic analyses of ...
Sachslehner AP, Lachner J, Mlitz V, Golabi B, Hess C, Sipos W, Eckhart L.   +6 more
europepmc   +2 more sources

Thermophilic whole‐cell degradation of polyethylene terephthalate using engineered Clostridium thermocellum

Microbial Biotechnology, Volume 14, Issue 2, Page 374-385, March 2021., 2021
Promising bioremediation strategies for plastics waste are of great importance and requirements. In our study, we constructed a recombinant Clostridium thermocellum strain expressing a secretory cutinase (LCC) as a thermophilic whole‐cell biocatalyst to degrade PET under high‐temperature condition (60°C).
Fei Yan, Ren Wei, Qiu Cui, Uwe T. Bornscheuer, Ya‐Jun Liu   +4 more
wiley   +1 more source

A Novel Acetivibrio cellulolyticus Anchoring Scaffoldin That Bears Divergent Cohesins [PDF]

Journal of Bacteriology, 2004
ABSTRACT Sequencing of a cellulosome-integrating gene cluster in Acetivibrio cellulolyticus was completed. The cluster contains four tandem scaffoldin genes ( scaA , scaB , scaC , and scaD ) bounded upstream and
Qi, Xu, Yoav, Barak, Rina, Kenig, Yuval, Shoham, Edward A, Bayer, Raphael, Lamed   +5 more
openaire   +2 more sources