Results 21 to 30 of about 1,033 (184)
Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome‐Based Synthetic Protein Scaffold [PDF]
Microbial Biotechnology, Volume 18, Issue 4, April 2025.The first three Escherichia coli glycolytic enzymes were converted to the docking enzyme mode and subsequently scaffolded. Increasing copies of the rate‐limiting enzyme boosted the overall performance. At lower enzyme densities, the proximity effects provided by scaffold‐colocalisation result in a higher product yield compared to free docking enzymes ...
Marte Elias +4 more
wiley +2 more sources
On the remarkable mechanostability of scaffoldins and the mechanical clamp motif [PDF]
Proceedings of the National Academy of Sciences, 2009 Protein mechanostability is a fundamental biological property that can only be measured by single-molecule manipulation techniques. Such studies have unveiled a variety of highly mechanostable modules (mainly of the Ig-like, β-sandwich type) in modular proteins subjected to mechanical stress from the cytoskeleton and the metazoan cell–cell interface ...
Valbuena, Alejandro +8 more
openaire +3 more sources
IQGAP1, AmotL2, and FKBP51 Scaffoldins in the Glioblastoma Microenvironment [PDF]
Journal of Histochemistry & Cytochemistry, 2019 Glioblastoma (GB) is the most frequently occurring and aggressive primary brain tumor. Glioma stem cells (GSCs) and astrocytoma cells are the predominant malignant cells occurring in GB besides a highly heterogeneous population of migrating, neovascularizing and infiltrating myeloid cells that forms a complex tumor microenvironment (TME).
Rotoli D +7 more
openaire +6 more sources
Cellulosomal Scaffoldin-Like Proteins from Ruminococcus flavefaciens [PDF]
Journal of Bacteriology, 2001 ABSTRACT Two tandem cellulosome-associated genes were identified in the cellulolytic rumen bacterium, Ruminococcus flavefaciens . The deduced gene products represent multimodular scaffoldin-related proteins (termed ScaA and ScaB), both of which include several copies of explicit cellulosome signature ...
SHIYOU DING +8 more
openaire +4 more sources
Journal of Computer Assisted Learning, Volume 39, Issue 5, Page 1652-1665, October 2023., 2023 Abstract Background Computer‐based scaffolding has been intensively used to facilitate students' self‐regulated learning (SRL). However, most previous studies investigated how computer‐based scaffoldings affected the cognitive aspect of SRL, such as knowledge gains and understanding levels. In contrast, more evidence is needed to examine the effects of
Tingting Wang +3 more
wiley +1 more source
Biotechnology and Bioengineering, Volume 120, Issue 8, Page 2253-2268, August 2023., 2023 Carbohydrate binding modules (CBMs) are noncatalytic domains that have been used to visualize distinct polysaccharides present in the plant cell wall. Here, engineered type‐A tandem CBMs have been characterized using pull‐down and kinetic assays.
Dharanidaran Jayachandran +7 more
wiley +1 more source
Impact of scaffoldin mechanostability on cellulosomal activity
Biomaterials Science, 2020 A low mechanostability cohesin between the two cellulosomal anchoring points lowers saccharification activity (top), in contrast to alternative configurations (bottom).
Albert Galera-Prat +5 more
openaire +4 more sources
Synthetic Multienzyme Assemblies for Natural Product Biosynthesis
ChemBioChem, Volume 24, Issue 6, March 14, 2023., 2023 Constructing multienzyme complexes has become an effective way to optimize metabolic flux and increase the titer of natural products in metabolic engineering recently. In this review, we summarize strategies for constructing synthetic multienzyme complexes in microbial cells and their applications in promoting natural product biosynthesis.
Min Liu +4 more
wiley +1 more source
Immunolocalization of Scaffoldin, a Trichohyalin‐Like Protein, in the Epidermis of the Chicken Embryo [PDF]
The Anatomical Record, 2014 ABSTRACTRecent comparative genomic studies have identified a chicken gene that codes for a trichohyalin‐like protein rich in arginine and glutamic acid termed scaffoldin. Immunocytochemistry and immunoelectron microscopy show that this protein is predominantly localized in periderm granules, subcellular structures present in the periderm of the ...
L, Alibardi, V, Mlitz, L, Eckhart
openaire +2 more sources
Microbial Biotechnology, Volume 16, Issue 2, Page 238-261, February 2023., 2023 Development of consolidated bioprocessing (CBP) of lignocellulose to butanol can potentially replace butanol production from non‐renewable sources at viable costs. Progress in CBP development has been obtained by combination of (hemi)cellulolytic and butanol‐producing features in a single strain (by metabolic engineering or protoplast fusion) or by ...
Angela Re, Roberto Mazzoli
wiley +1 more source