Results 11 to 20 of about 754 (153)

Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature [PDF]

mBio, 2016
Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes. The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
Johanna Stern, Sarah Moraïs, Raphael Lamed, Edward A. Bayer   +3 more
doaj   +3 more sources

Cell‐surface binding domains from Clostridium cellulovorans can be used for surface display of cellulosomal scaffoldins in Lactococcus lactis [PDF]

Biotechnology Journal, Volume 16, Issue 8, August 2021., 2021
Engineering microbial strains combining efficient lignocellulose metabolization and high‐value chemical production is a cutting‐edge strategy towards cost‐sustainable 2nd generation biorefining. Here, Lactococcus lactis (efficient lactic acid producer) was engineered with synthetic scaffoldins for assembly of secreted and cell‐surface displayed ...
Loredana Tarraran, Chiara Gandini, Anna Luganini, Roberto Mazzoli   +3 more
wiley   +5 more sources

Rapid adaptation for fibre degradation by changes in plasmid stoichiometry within Lactobacillus plantarum at the synthetic community level

Microbial Biotechnology, 2020
Summary The multi‐enzyme cellulosome complex can mediate the valorization of lignocellulosic biomass into soluble sugars that can serve in the production of biofuels and valuable products.
Yonit Ben‐David, Sarah Moraïs, Edward A. Bayer, Itzhak Mizrahi   +3 more
doaj   +2 more sources

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction [PDF]

Scientific Reports, 2018
Cellulosomes are highly sophisticated molecular nanomachines that participate in the deconstruction of complex polysaccharides, notably cellulose and hemicellulose.
Pedro Bule, Virgínia M. R. Pires, Victor D. Alves, Ana Luísa Carvalho, José A. M. Prates, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Ilit Noach, Edward A. Bayer, Shabir Najmudin, Carlos M. G. A. Fontes   +11 more
doaj   +4 more sources

A Scaffoldomics Platform for Modular In Vivo Enzyme Colocalisation and Its Application to Naringenin Biosynthesis. [PDF]

Microb Biotechnol
A hierarchical assembly system was established in which the coding sequences for four docking enzymes and a scaffold are co‐assembled onto a single plasmid, thereby establishing a ‘scaffoldomics’ platform for the optimisation of multi‐enzyme biosynthetic pathways.
Elias M, De Paepe B, Vanderstraeten J, Lamote B, De Mey M, Briers Y.   +5 more
europepmc   +2 more sources

On the remarkable mechanostability of scaffoldins and the mechanical clamp motif [PDF]

Proceedings of the National Academy of Sciences, 2009
Protein mechanostability is a fundamental biological property that can only be measured by single-molecule manipulation techniques. Such studies have unveiled a variety of highly mechanostable modules (mainly of the Ig-like, β-sandwich type) in modular proteins subjected to mechanical stress from the cytoskeleton and the metazoan cell–cell interface ...
Valbuena, Alejandro, Oroz, Javier, Hervás, Rubén, Vera, Andrés M., Rodríguez, David, Menéndez, Margarita, Sulkowska, Joanna I., Cieplak, Marek, Carrión-Vázquez, Mariano Sixto   +8 more
openaire   +3 more sources

IQGAP1, AmotL2, and FKBP51 Scaffoldins in the Glioblastoma Microenvironment [PDF]

Journal of Histochemistry & Cytochemistry, 2019
Glioblastoma (GB) is the most frequently occurring and aggressive primary brain tumor. Glioma stem cells (GSCs) and astrocytoma cells are the predominant malignant cells occurring in GB besides a highly heterogeneous population of migrating, neovascularizing and infiltrating myeloid cells that forms a complex tumor microenvironment (TME).
Rotoli D, Morales M, Maeso MDC, Avila J, PerezRodriguez ND, Mobasheri A, van Noorden CJF, MartinVasallo P   +7 more
openaire   +6 more sources

Cellulosomal Scaffoldin-Like Proteins from Ruminococcus flavefaciens [PDF]

Journal of Bacteriology, 2001
ABSTRACT Two tandem cellulosome-associated genes were identified in the cellulolytic rumen bacterium, Ruminococcus flavefaciens . The deduced gene products represent multimodular scaffoldin-related proteins (termed ScaA and ScaB), both of which include several copies of explicit cellulosome signature ...
SHIYOU DING, MARCO T RINCON, RAPHAEL LAMED, JENNIFER C MARTIN, SHEILA I MCCRAE, VINCENZO AURILIA, YUVAL SHOHAM, EDWARD A BAYER, HARRY J FLINT   +8 more
openaire   +4 more sources

Computer‐based scaffoldings influence students' metacognitive monitoring and problem‐solving efficiency in an intelligent tutoring system

Journal of Computer Assisted Learning, Volume 39, Issue 5, Page 1652-1665, October 2023., 2023
Abstract Background Computer‐based scaffolding has been intensively used to facilitate students' self‐regulated learning (SRL). However, most previous studies investigated how computer‐based scaffoldings affected the cognitive aspect of SRL, such as knowledge gains and understanding levels. In contrast, more evidence is needed to examine the effects of
Tingting Wang, Juan Zheng, Chengyi Tan, Susanne P. Lajoie   +3 more
wiley   +1 more source

Impact of scaffoldin mechanostability on cellulosomal activity

Biomaterials Science, 2020
A low mechanostability cohesin between the two cellulosomal anchoring points lowers saccharification activity (top), in contrast to alternative configurations (bottom).
Albert Galera-Prat, Andrés M. Vera, Sarah Moraïs, Yael Vazana, Edward A. Bayer, Mariano Carrión-Vázquez   +5 more
openaire   +4 more sources