Results 91 to 100 of about 403,157 (288)

Characterization of Central Carbon Metabolism of Streptococcus pneumoniae by Isotopologue Profiling [PDF]

Journal of Biological Chemistry, 2012
The metabolism of Streptococcus pneumoniae was studied by isotopologue profiling after bacterial cultivation in chemically defined medium supplemented with [U-(13)C(6)]- or [1,2-(13)C(2)]glucose. GC/MS analysis of protein-derived amino acids showed lack of (13)C label in amino acids that were also essential for pneumococcal growth.
Hartel, T., Eylert, E., Schulz, C., Petruschka, L., Gierok, P., Grubmuller, S., Lalk, M., Eisenreich, W., Hammerschmidt, S.   +8 more
openaire   +2 more sources

Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield [PDF]

, 2020
Open Access Article; Published online: 17 Jan 2020Cassava is an important staple crop in sub‐Saharan Africa, due to its high productivity even on nutrient poor soils.
Fernie, A.R., Gisel, A., Klemens, P.A.W., Ludewig, F., Morales, N., Muller, L.A., Neuhaus, H.E., Obata, T., Rosado-Souza, L., Schlereth, A., Sonnewald, U., Stavolone, L., Stitt, M., Zeeman, S.C., Zierer, W.   +14 more
core   +3 more sources

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Mapping carbon utilization pathways in Histoplasma capsulatum through 13C-metabolic flux analysis

mSystems
Histoplasma capsulatum is a human fungal pathogen that survives and proliferates within phagocytic immune cells. To sustain growth in the nutrient-limited phagosome environment, the pathogenic yeast scavenges available carbon sources, which must be ...
Adrian Heckart, Jean-Christophe Cocuron, Stephanie C. Ray, Gabriella F. Matheny, Chad A. Rappleye, Ana P. Alonso   +5 more
doaj   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

Organising metabolic networks: cycles in flux distributions [PDF]

, 2009
Metabolic networks are among the most widely studied biological systems. The topology and interconnections of metabolic reactions have been well described for many species, but are not sufficient to understand how their activity is regulated in living ...
Jean-Marc Schwartz, Marcelo T. dos Santos, Maurí, Sebastiá   +3 more
core   +2 more sources

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Monte-Carlo Modeling of the Central Carbon Metabolism of Lactococcus lactis: Insights into Metabolic Regulation

PLoS ONE, 2014
Metabolic pathways are complex dynamic systems whose response to perturbations and environmental challenges are governed by multiple interdependencies between enzyme properties, reactions rates, and substrate levels. Understanding the dynamics arising from such a network can be greatly enhanced by the construction of a computational model that embodies
Murabito, E., Verma, M., Bekker, M., Bellomo, D., Westerhoff, H.V., Teusink, B., Steuer, R.   +6 more
openaire   +8 more sources

Differences in Fatty Acid and Central Carbon Metabolite Distribution among Different Tissues of Alfalfa–Rhizobia Symbiotic System

Agronomy
Fatty acid and central carbon metabolism are crucial energy metabolism reactions. However, to date, few studies have examined their distribution characteristics within the alfalfa–rhizobia symbiotic system.
Bao-Fu Lu, Wen-Juan Kang, Shang-Li Shi, Jian Guan, Fang Jing, Bei Wu   +5 more
doaj   +1 more source

Engineering CHO cells for improved central carbon and energy metabolism [PDF]

BMC Proceedings, 2011
Background Investigations have shown animal cell cultures’ performance, in terms of cell proliferation and production of recombinant protein, are negatively affected by both lactate’s concentration and its specific production rate. In a previous work, we determined that lactate production was caused by pyruvate accumulation due to its high synthesis ...
Wilkens Camila A, Gerdtzen Ziomara P
doaj   +3 more sources